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Exploring an ultra-sensitive electrochemiluminescence monitoring strategy for SARS-CoV-2 using hairpin-assisted cycling and dumbbell hybridization chain amplification

Rapid and accurate discrimination of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an available approach to implement a rapid diagnosis of the coronavirus disease 2019 (COVID-19). Here we fully exploited the cleavage properties of exonuclease III (Exo III) and hairpin DNA-assisted...

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Autores principales: Wu, Biaoliang, Wen, Xiaoqing, Cui, Jiuying, Qin, Xiaojie, Li, Zheng, Gong, Yuanxun, Tang, Qianli, Liao, Xianjiu, Fan, Zhenqiang, Zhang, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9423879/
http://dx.doi.org/10.1016/j.jhazmat.2022.129868
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author Wu, Biaoliang
Wen, Xiaoqing
Cui, Jiuying
Qin, Xiaojie
Li, Zheng
Gong, Yuanxun
Tang, Qianli
Liao, Xianjiu
Fan, Zhenqiang
Zhang, Kai
author_facet Wu, Biaoliang
Wen, Xiaoqing
Cui, Jiuying
Qin, Xiaojie
Li, Zheng
Gong, Yuanxun
Tang, Qianli
Liao, Xianjiu
Fan, Zhenqiang
Zhang, Kai
author_sort Wu, Biaoliang
collection PubMed
description Rapid and accurate discrimination of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an available approach to implement a rapid diagnosis of the coronavirus disease 2019 (COVID-19). Here we fully exploited the cleavage properties of exonuclease III (Exo III) and hairpin DNA-assisted target cycling technology to generate bulk single-stranded DNA (ssDNA) that was employed to facilitate the constitution of a three-way junction structure on polymetallic particle (Ag-Au NPs) and Ti(3)C(2) (Ti(3)C(2) @Ag-Au) complexes. Ag-Au NPs presented favorable stability without adding extra stabilizers, demonstrating the potential value of Ag-Au NPs as an alternative to Au NPs in the field of bioanalysis. Uppon the three-way junction structure, the dumbbell hybridization chain amplification (DHCA) was occurred which generated DNA nanostructure with tight conformation. Target cycling and DHCA reactions improved the electrochemiluminescence (ECL) signal, which dramatically advanced the assay sensitivity of SARS-CoV-2 (0.59 fM). Moreover, our strategy remained to demonstrate favorable specificity and repeatability in environmental conditions and real human serum samples.
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spelling pubmed-94238792022-08-30 Exploring an ultra-sensitive electrochemiluminescence monitoring strategy for SARS-CoV-2 using hairpin-assisted cycling and dumbbell hybridization chain amplification Wu, Biaoliang Wen, Xiaoqing Cui, Jiuying Qin, Xiaojie Li, Zheng Gong, Yuanxun Tang, Qianli Liao, Xianjiu Fan, Zhenqiang Zhang, Kai J Hazard Mater Research Paper Rapid and accurate discrimination of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an available approach to implement a rapid diagnosis of the coronavirus disease 2019 (COVID-19). Here we fully exploited the cleavage properties of exonuclease III (Exo III) and hairpin DNA-assisted target cycling technology to generate bulk single-stranded DNA (ssDNA) that was employed to facilitate the constitution of a three-way junction structure on polymetallic particle (Ag-Au NPs) and Ti(3)C(2) (Ti(3)C(2) @Ag-Au) complexes. Ag-Au NPs presented favorable stability without adding extra stabilizers, demonstrating the potential value of Ag-Au NPs as an alternative to Au NPs in the field of bioanalysis. Uppon the three-way junction structure, the dumbbell hybridization chain amplification (DHCA) was occurred which generated DNA nanostructure with tight conformation. Target cycling and DHCA reactions improved the electrochemiluminescence (ECL) signal, which dramatically advanced the assay sensitivity of SARS-CoV-2 (0.59 fM). Moreover, our strategy remained to demonstrate favorable specificity and repeatability in environmental conditions and real human serum samples. Elsevier B.V. 2022-10-15 2022-08-30 /pmc/articles/PMC9423879/ http://dx.doi.org/10.1016/j.jhazmat.2022.129868 Text en © 2022 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Research Paper
Wu, Biaoliang
Wen, Xiaoqing
Cui, Jiuying
Qin, Xiaojie
Li, Zheng
Gong, Yuanxun
Tang, Qianli
Liao, Xianjiu
Fan, Zhenqiang
Zhang, Kai
Exploring an ultra-sensitive electrochemiluminescence monitoring strategy for SARS-CoV-2 using hairpin-assisted cycling and dumbbell hybridization chain amplification
title Exploring an ultra-sensitive electrochemiluminescence monitoring strategy for SARS-CoV-2 using hairpin-assisted cycling and dumbbell hybridization chain amplification
title_full Exploring an ultra-sensitive electrochemiluminescence monitoring strategy for SARS-CoV-2 using hairpin-assisted cycling and dumbbell hybridization chain amplification
title_fullStr Exploring an ultra-sensitive electrochemiluminescence monitoring strategy for SARS-CoV-2 using hairpin-assisted cycling and dumbbell hybridization chain amplification
title_full_unstemmed Exploring an ultra-sensitive electrochemiluminescence monitoring strategy for SARS-CoV-2 using hairpin-assisted cycling and dumbbell hybridization chain amplification
title_short Exploring an ultra-sensitive electrochemiluminescence monitoring strategy for SARS-CoV-2 using hairpin-assisted cycling and dumbbell hybridization chain amplification
title_sort exploring an ultra-sensitive electrochemiluminescence monitoring strategy for sars-cov-2 using hairpin-assisted cycling and dumbbell hybridization chain amplification
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9423879/
http://dx.doi.org/10.1016/j.jhazmat.2022.129868
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