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m(6)A Regulator-Based Exosomal Gene Methylation Modification Patterns Identify Distinct Microenvironment Characterization and Predict Immunotherapeutic Responses in Colon Cancer
Recent studies have highlighted the biological significance of exosomes and m(6)A modifications in immunity. Nonetheless, it remains unclear whether the m(6)A modification gene in exosomes of body fluid has potential roles in the tumor microenvironment (TME). Herein, we identified three different m(...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9423980/ https://www.ncbi.nlm.nih.gov/pubmed/36046691 http://dx.doi.org/10.1155/2022/9451480 |
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author | Zheng, Junjie Feng, Panpan Chen, Dawei Zhang, Cuiyu Ji, Yuge Liu, Yanting Fan, Xiaohua Li, Jingxin |
author_facet | Zheng, Junjie Feng, Panpan Chen, Dawei Zhang, Cuiyu Ji, Yuge Liu, Yanting Fan, Xiaohua Li, Jingxin |
author_sort | Zheng, Junjie |
collection | PubMed |
description | Recent studies have highlighted the biological significance of exosomes and m(6)A modifications in immunity. Nonetheless, it remains unclear whether the m(6)A modification gene in exosomes of body fluid has potential roles in the tumor microenvironment (TME). Herein, we identified three different m(6)A-related exosomal gene modification patterns based on 59 m(6)A-related exosomal genes, which instructed distinguishing characteristics of TME in colon cancer (CC). We demonstrated that these patterns could predict the stage of tumor inflammation, subtypes, genetic variation, and patient prognosis. Furthermore, we developed a scoring mode—m(6)A-related exosomal gene score (MREGS)—by detecting the level of m(6)A modification in exosomes to classify immune phenotypes. Low MREGS, characterized by prominent survival and immune activation, was linked to a better response to anti-PDL1 immunotherapy. In contrast, the higher MREGS group displayed remarkable stromal activation, high activity of innate immunocytes, and a lower survival rate. Hence, this work provides a novel approach for evaluating TME cell infiltration in colon cancer and guiding more effective immunotherapy strategies. |
format | Online Article Text |
id | pubmed-9423980 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-94239802022-08-30 m(6)A Regulator-Based Exosomal Gene Methylation Modification Patterns Identify Distinct Microenvironment Characterization and Predict Immunotherapeutic Responses in Colon Cancer Zheng, Junjie Feng, Panpan Chen, Dawei Zhang, Cuiyu Ji, Yuge Liu, Yanting Fan, Xiaohua Li, Jingxin Oxid Med Cell Longev Research Article Recent studies have highlighted the biological significance of exosomes and m(6)A modifications in immunity. Nonetheless, it remains unclear whether the m(6)A modification gene in exosomes of body fluid has potential roles in the tumor microenvironment (TME). Herein, we identified three different m(6)A-related exosomal gene modification patterns based on 59 m(6)A-related exosomal genes, which instructed distinguishing characteristics of TME in colon cancer (CC). We demonstrated that these patterns could predict the stage of tumor inflammation, subtypes, genetic variation, and patient prognosis. Furthermore, we developed a scoring mode—m(6)A-related exosomal gene score (MREGS)—by detecting the level of m(6)A modification in exosomes to classify immune phenotypes. Low MREGS, characterized by prominent survival and immune activation, was linked to a better response to anti-PDL1 immunotherapy. In contrast, the higher MREGS group displayed remarkable stromal activation, high activity of innate immunocytes, and a lower survival rate. Hence, this work provides a novel approach for evaluating TME cell infiltration in colon cancer and guiding more effective immunotherapy strategies. Hindawi 2022-08-22 /pmc/articles/PMC9423980/ /pubmed/36046691 http://dx.doi.org/10.1155/2022/9451480 Text en Copyright © 2022 Junjie Zheng et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zheng, Junjie Feng, Panpan Chen, Dawei Zhang, Cuiyu Ji, Yuge Liu, Yanting Fan, Xiaohua Li, Jingxin m(6)A Regulator-Based Exosomal Gene Methylation Modification Patterns Identify Distinct Microenvironment Characterization and Predict Immunotherapeutic Responses in Colon Cancer |
title | m(6)A Regulator-Based Exosomal Gene Methylation Modification Patterns Identify Distinct Microenvironment Characterization and Predict Immunotherapeutic Responses in Colon Cancer |
title_full | m(6)A Regulator-Based Exosomal Gene Methylation Modification Patterns Identify Distinct Microenvironment Characterization and Predict Immunotherapeutic Responses in Colon Cancer |
title_fullStr | m(6)A Regulator-Based Exosomal Gene Methylation Modification Patterns Identify Distinct Microenvironment Characterization and Predict Immunotherapeutic Responses in Colon Cancer |
title_full_unstemmed | m(6)A Regulator-Based Exosomal Gene Methylation Modification Patterns Identify Distinct Microenvironment Characterization and Predict Immunotherapeutic Responses in Colon Cancer |
title_short | m(6)A Regulator-Based Exosomal Gene Methylation Modification Patterns Identify Distinct Microenvironment Characterization and Predict Immunotherapeutic Responses in Colon Cancer |
title_sort | m(6)a regulator-based exosomal gene methylation modification patterns identify distinct microenvironment characterization and predict immunotherapeutic responses in colon cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9423980/ https://www.ncbi.nlm.nih.gov/pubmed/36046691 http://dx.doi.org/10.1155/2022/9451480 |
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