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Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics

BACKGROUND: Naolingsu capsule (NLSC) is a well-known traditional Chinese medicine (TCM) prescription in China. It is widely used to treat neurasthenia, insomnia, cardiovascular and cerebrovascular disease, and other diseases. However, its inalienable chemical groups have not been carried out. METHOD...

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Autores principales: Xu, Lili, Jiao, Yang, Cui, Weiliang, Wang, Bing, Guo, Dongxiao, Xue, Fei, Mu, Xiangrong, Li, Huifen, Lin, Yongqiang, Lin, Huibin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9424029/
https://www.ncbi.nlm.nih.gov/pubmed/36046660
http://dx.doi.org/10.1155/2022/1429074
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author Xu, Lili
Jiao, Yang
Cui, Weiliang
Wang, Bing
Guo, Dongxiao
Xue, Fei
Mu, Xiangrong
Li, Huifen
Lin, Yongqiang
Lin, Huibin
author_facet Xu, Lili
Jiao, Yang
Cui, Weiliang
Wang, Bing
Guo, Dongxiao
Xue, Fei
Mu, Xiangrong
Li, Huifen
Lin, Yongqiang
Lin, Huibin
author_sort Xu, Lili
collection PubMed
description BACKGROUND: Naolingsu capsule (NLSC) is a well-known traditional Chinese medicine (TCM) prescription in China. It is widely used to treat neurasthenia, insomnia, cardiovascular and cerebrovascular disease, and other diseases. However, its inalienable chemical groups have not been carried out. METHODS: We first established the nontargeted investigation based on fingerprinting coupled with UHPLC-Q/TOF-MS/MS. Second, the quantitative methods based on HPLC-DAD and LC-MS/MS were connected to the synchronous quantitative assurance of eleven and fourteen marker compounds. Finally, the quantitative information was processed with SIMCA-P for differentiating the distinctive bunches of samples to screen the foremost appropriate chemical markers. RESULTS: The similarity of HPLC fingerprints of 24 batches of NLSC samples was 0.645–0.992. In total, 37 flavonoids, 21 organic acids, 22 lignans, 13 saponins, and 20 other compounds were recognized in NLSC by the UHPLC-Q/TOF-MS/MS method. The quantitative determination was approved for linearity, discovery limits, accuracy, repeatability, soundness, and precision. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) models accomplished the great classification of the samples from the five enterprises, respectively. Rehmannioside D (RD), methylophiopogonanone A (MPA), 3,6′-disinapoyl sucrose (DS), schisandrin B (SSB), epimedin C (EC), icariin (ICA), and jujuboside B (JB) were considered as the potential chemical markers for NLSC quality control. CONCLUSION: The experimental results illustrated that the combinative strategy was valuable for quick pharmaceutical quality assessment, which can potentially differentiate the origin, decide the realness, and assess the overall quality of the formulation.
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spelling pubmed-94240292022-08-30 Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics Xu, Lili Jiao, Yang Cui, Weiliang Wang, Bing Guo, Dongxiao Xue, Fei Mu, Xiangrong Li, Huifen Lin, Yongqiang Lin, Huibin J Anal Methods Chem Research Article BACKGROUND: Naolingsu capsule (NLSC) is a well-known traditional Chinese medicine (TCM) prescription in China. It is widely used to treat neurasthenia, insomnia, cardiovascular and cerebrovascular disease, and other diseases. However, its inalienable chemical groups have not been carried out. METHODS: We first established the nontargeted investigation based on fingerprinting coupled with UHPLC-Q/TOF-MS/MS. Second, the quantitative methods based on HPLC-DAD and LC-MS/MS were connected to the synchronous quantitative assurance of eleven and fourteen marker compounds. Finally, the quantitative information was processed with SIMCA-P for differentiating the distinctive bunches of samples to screen the foremost appropriate chemical markers. RESULTS: The similarity of HPLC fingerprints of 24 batches of NLSC samples was 0.645–0.992. In total, 37 flavonoids, 21 organic acids, 22 lignans, 13 saponins, and 20 other compounds were recognized in NLSC by the UHPLC-Q/TOF-MS/MS method. The quantitative determination was approved for linearity, discovery limits, accuracy, repeatability, soundness, and precision. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) models accomplished the great classification of the samples from the five enterprises, respectively. Rehmannioside D (RD), methylophiopogonanone A (MPA), 3,6′-disinapoyl sucrose (DS), schisandrin B (SSB), epimedin C (EC), icariin (ICA), and jujuboside B (JB) were considered as the potential chemical markers for NLSC quality control. CONCLUSION: The experimental results illustrated that the combinative strategy was valuable for quick pharmaceutical quality assessment, which can potentially differentiate the origin, decide the realness, and assess the overall quality of the formulation. Hindawi 2022-08-22 /pmc/articles/PMC9424029/ /pubmed/36046660 http://dx.doi.org/10.1155/2022/1429074 Text en Copyright © 2022 Lili Xu et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Xu, Lili
Jiao, Yang
Cui, Weiliang
Wang, Bing
Guo, Dongxiao
Xue, Fei
Mu, Xiangrong
Li, Huifen
Lin, Yongqiang
Lin, Huibin
Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics
title Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics
title_full Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics
title_fullStr Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics
title_full_unstemmed Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics
title_short Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics
title_sort quality evaluation of traditional chinese medicine prescription in naolingsu capsule based on combinative method of fingerprint, quantitative determination, and chemometrics
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9424029/
https://www.ncbi.nlm.nih.gov/pubmed/36046660
http://dx.doi.org/10.1155/2022/1429074
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