Cargando…

High-efficiency decomposition of eggshell membrane by a keratinase from Meiothermus taiwanensis

Eggshell membrane (ESM), a plentiful biological waste, consists of collagen-like proteins and glycosaminoglycans (GAGs) such as hyaluronic acid (HA). Here we used a keratinase (oeMtaker)-mediated system to decompose ESM. The best reaction condition was established by incubating the solution containi...

Descripción completa

Detalles Bibliográficos
Autores principales: Lien, Ya-Chu, Lai, Shu-Jung, Lin, Chai-Yi, Wong, Ken-Pei, Chang, Matt S., Wu, Shih-Hsiung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9424195/
https://www.ncbi.nlm.nih.gov/pubmed/36038640
http://dx.doi.org/10.1038/s41598-022-18474-4
_version_ 1784778187853529088
author Lien, Ya-Chu
Lai, Shu-Jung
Lin, Chai-Yi
Wong, Ken-Pei
Chang, Matt S.
Wu, Shih-Hsiung
author_facet Lien, Ya-Chu
Lai, Shu-Jung
Lin, Chai-Yi
Wong, Ken-Pei
Chang, Matt S.
Wu, Shih-Hsiung
author_sort Lien, Ya-Chu
collection PubMed
description Eggshell membrane (ESM), a plentiful biological waste, consists of collagen-like proteins and glycosaminoglycans (GAGs) such as hyaluronic acid (HA). Here we used a keratinase (oeMtaker)-mediated system to decompose ESM. The best reaction condition was established by incubating the solution containing oeMtaker, sodium sulfite, and ESM with a weight ratio of 1:120:600. ESM enzymatic hydrolysate (ESM-EH) showed a high proportion of essential amino acids and type X collagen peptides with 963–2259 Da molecular weights. The amounts of GAGs and sulfated GAGs in ESM-EH were quantified as 6.4% and 0.7%, respectively. The precipitated polysaccharides with an average molecular weight of 1300–1700 kDa showed an immunomodulatory activity by stimulating pro-inflammatory cytokines (IL-6 and TNF-α) production. In addition, a microorganism-based system was established to hydrolyze ESM by Meiothermus taiwanensis WR-220. The amounts of GAGs and sulfated GAGs in the system were quantified as 0.9% and 0.1%, respectively. Based on our pre-pilot tests, the system shows great promise in developing into a low-cost and high-performance process. These results indicate that the keratinase-mediated system could hydrolyze ESM more efficiently and produce more bioactive substances than ever for therapeutical applications and dietary supplements.
format Online
Article
Text
id pubmed-9424195
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-94241952022-08-31 High-efficiency decomposition of eggshell membrane by a keratinase from Meiothermus taiwanensis Lien, Ya-Chu Lai, Shu-Jung Lin, Chai-Yi Wong, Ken-Pei Chang, Matt S. Wu, Shih-Hsiung Sci Rep Article Eggshell membrane (ESM), a plentiful biological waste, consists of collagen-like proteins and glycosaminoglycans (GAGs) such as hyaluronic acid (HA). Here we used a keratinase (oeMtaker)-mediated system to decompose ESM. The best reaction condition was established by incubating the solution containing oeMtaker, sodium sulfite, and ESM with a weight ratio of 1:120:600. ESM enzymatic hydrolysate (ESM-EH) showed a high proportion of essential amino acids and type X collagen peptides with 963–2259 Da molecular weights. The amounts of GAGs and sulfated GAGs in ESM-EH were quantified as 6.4% and 0.7%, respectively. The precipitated polysaccharides with an average molecular weight of 1300–1700 kDa showed an immunomodulatory activity by stimulating pro-inflammatory cytokines (IL-6 and TNF-α) production. In addition, a microorganism-based system was established to hydrolyze ESM by Meiothermus taiwanensis WR-220. The amounts of GAGs and sulfated GAGs in the system were quantified as 0.9% and 0.1%, respectively. Based on our pre-pilot tests, the system shows great promise in developing into a low-cost and high-performance process. These results indicate that the keratinase-mediated system could hydrolyze ESM more efficiently and produce more bioactive substances than ever for therapeutical applications and dietary supplements. Nature Publishing Group UK 2022-08-29 /pmc/articles/PMC9424195/ /pubmed/36038640 http://dx.doi.org/10.1038/s41598-022-18474-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Lien, Ya-Chu
Lai, Shu-Jung
Lin, Chai-Yi
Wong, Ken-Pei
Chang, Matt S.
Wu, Shih-Hsiung
High-efficiency decomposition of eggshell membrane by a keratinase from Meiothermus taiwanensis
title High-efficiency decomposition of eggshell membrane by a keratinase from Meiothermus taiwanensis
title_full High-efficiency decomposition of eggshell membrane by a keratinase from Meiothermus taiwanensis
title_fullStr High-efficiency decomposition of eggshell membrane by a keratinase from Meiothermus taiwanensis
title_full_unstemmed High-efficiency decomposition of eggshell membrane by a keratinase from Meiothermus taiwanensis
title_short High-efficiency decomposition of eggshell membrane by a keratinase from Meiothermus taiwanensis
title_sort high-efficiency decomposition of eggshell membrane by a keratinase from meiothermus taiwanensis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9424195/
https://www.ncbi.nlm.nih.gov/pubmed/36038640
http://dx.doi.org/10.1038/s41598-022-18474-4
work_keys_str_mv AT lienyachu highefficiencydecompositionofeggshellmembranebyakeratinasefrommeiothermustaiwanensis
AT laishujung highefficiencydecompositionofeggshellmembranebyakeratinasefrommeiothermustaiwanensis
AT linchaiyi highefficiencydecompositionofeggshellmembranebyakeratinasefrommeiothermustaiwanensis
AT wongkenpei highefficiencydecompositionofeggshellmembranebyakeratinasefrommeiothermustaiwanensis
AT changmatts highefficiencydecompositionofeggshellmembranebyakeratinasefrommeiothermustaiwanensis
AT wushihhsiung highefficiencydecompositionofeggshellmembranebyakeratinasefrommeiothermustaiwanensis