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One of the isoamylase isoforms, CMI294C, is required for semi-amylopectin synthesis in the rhodophyte Cyanidioschyzon merolae

Most rhodophytes synthesize semi-amylopectin as a storage polysaccharide, whereas some species in the most primitive class (Cyanidiophyceae) make glycogen. To know the roles of isoamylases in semi-amylopectin synthesis, we investigated the effects of isoamylase gene (CMI294C and CMS197C)-deficiencie...

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Autores principales: Maeno, Toshiki, Yamakawa, Yuki, Takiyasu, Yohei, Miyauchi, Hiroki, Nakamura, Yasunori, Ono, Masami, Ozaki, Noriaki, Utsumi, Yoshinori, Cenci, Ugo, Colleoni, Christophe, Ball, Steven, Tsuzuki, Mikio, Fujiwara, Shoko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9424615/
https://www.ncbi.nlm.nih.gov/pubmed/36051298
http://dx.doi.org/10.3389/fpls.2022.967165
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author Maeno, Toshiki
Yamakawa, Yuki
Takiyasu, Yohei
Miyauchi, Hiroki
Nakamura, Yasunori
Ono, Masami
Ozaki, Noriaki
Utsumi, Yoshinori
Cenci, Ugo
Colleoni, Christophe
Ball, Steven
Tsuzuki, Mikio
Fujiwara, Shoko
author_facet Maeno, Toshiki
Yamakawa, Yuki
Takiyasu, Yohei
Miyauchi, Hiroki
Nakamura, Yasunori
Ono, Masami
Ozaki, Noriaki
Utsumi, Yoshinori
Cenci, Ugo
Colleoni, Christophe
Ball, Steven
Tsuzuki, Mikio
Fujiwara, Shoko
author_sort Maeno, Toshiki
collection PubMed
description Most rhodophytes synthesize semi-amylopectin as a storage polysaccharide, whereas some species in the most primitive class (Cyanidiophyceae) make glycogen. To know the roles of isoamylases in semi-amylopectin synthesis, we investigated the effects of isoamylase gene (CMI294C and CMS197C)-deficiencies on semi-amylopectin molecular structure and starch granule morphology in Cyanidioschyzon merolae (Cyanidiophyceae). Semi-amylopectin content in a CMS197C-disruption mutant (ΔCMS197C) was not significantly different from that in the control strain, while that in a CMI294C-disruption mutant (ΔCMI294C) was much lower than those in the control strain, suggesting that CMI294C is essential for semi-amylopectin synthesis. Scanning electron microscopy showed that the ΔCMI294C strain contained smaller starch granules, while the ΔCMS197C strain had normal size, but donut-shaped granules, unlike those of the control strain. Although the chain length distribution of starch from the control strain displayed a semi-amylopectin pattern with a peak around degree of polymerization (DP) 11–13, differences in chain length profiles revealed that the ΔCMS197C strain has more short chains (DP of 3 and 4) than the control strain, while the ΔCMI294C strain has more long chains (DP ≥12). These findings suggest that CMI294C-type isoamylase, which can debranch a wide range of chains, probably plays an important role in semi-amylopectin synthesis unique in the Rhodophyta.
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spelling pubmed-94246152022-08-31 One of the isoamylase isoforms, CMI294C, is required for semi-amylopectin synthesis in the rhodophyte Cyanidioschyzon merolae Maeno, Toshiki Yamakawa, Yuki Takiyasu, Yohei Miyauchi, Hiroki Nakamura, Yasunori Ono, Masami Ozaki, Noriaki Utsumi, Yoshinori Cenci, Ugo Colleoni, Christophe Ball, Steven Tsuzuki, Mikio Fujiwara, Shoko Front Plant Sci Plant Science Most rhodophytes synthesize semi-amylopectin as a storage polysaccharide, whereas some species in the most primitive class (Cyanidiophyceae) make glycogen. To know the roles of isoamylases in semi-amylopectin synthesis, we investigated the effects of isoamylase gene (CMI294C and CMS197C)-deficiencies on semi-amylopectin molecular structure and starch granule morphology in Cyanidioschyzon merolae (Cyanidiophyceae). Semi-amylopectin content in a CMS197C-disruption mutant (ΔCMS197C) was not significantly different from that in the control strain, while that in a CMI294C-disruption mutant (ΔCMI294C) was much lower than those in the control strain, suggesting that CMI294C is essential for semi-amylopectin synthesis. Scanning electron microscopy showed that the ΔCMI294C strain contained smaller starch granules, while the ΔCMS197C strain had normal size, but donut-shaped granules, unlike those of the control strain. Although the chain length distribution of starch from the control strain displayed a semi-amylopectin pattern with a peak around degree of polymerization (DP) 11–13, differences in chain length profiles revealed that the ΔCMS197C strain has more short chains (DP of 3 and 4) than the control strain, while the ΔCMI294C strain has more long chains (DP ≥12). These findings suggest that CMI294C-type isoamylase, which can debranch a wide range of chains, probably plays an important role in semi-amylopectin synthesis unique in the Rhodophyta. Frontiers Media S.A. 2022-08-16 /pmc/articles/PMC9424615/ /pubmed/36051298 http://dx.doi.org/10.3389/fpls.2022.967165 Text en Copyright © 2022 Maeno, Yamakawa, Takiyasu, Miyauchi, Nakamura, Ono, Ozaki, Utsumi, Cenci, Colleoni, Ball, Tsuzuki and Fujiwara. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Maeno, Toshiki
Yamakawa, Yuki
Takiyasu, Yohei
Miyauchi, Hiroki
Nakamura, Yasunori
Ono, Masami
Ozaki, Noriaki
Utsumi, Yoshinori
Cenci, Ugo
Colleoni, Christophe
Ball, Steven
Tsuzuki, Mikio
Fujiwara, Shoko
One of the isoamylase isoforms, CMI294C, is required for semi-amylopectin synthesis in the rhodophyte Cyanidioschyzon merolae
title One of the isoamylase isoforms, CMI294C, is required for semi-amylopectin synthesis in the rhodophyte Cyanidioschyzon merolae
title_full One of the isoamylase isoforms, CMI294C, is required for semi-amylopectin synthesis in the rhodophyte Cyanidioschyzon merolae
title_fullStr One of the isoamylase isoforms, CMI294C, is required for semi-amylopectin synthesis in the rhodophyte Cyanidioschyzon merolae
title_full_unstemmed One of the isoamylase isoforms, CMI294C, is required for semi-amylopectin synthesis in the rhodophyte Cyanidioschyzon merolae
title_short One of the isoamylase isoforms, CMI294C, is required for semi-amylopectin synthesis in the rhodophyte Cyanidioschyzon merolae
title_sort one of the isoamylase isoforms, cmi294c, is required for semi-amylopectin synthesis in the rhodophyte cyanidioschyzon merolae
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9424615/
https://www.ncbi.nlm.nih.gov/pubmed/36051298
http://dx.doi.org/10.3389/fpls.2022.967165
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