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Quantitative proteomics on the cerebrospinal fluid of hydrocephalus in neonatal bacterial meningitis

OBJECTIVE: Hydrocephalus in bacterial meningitis (BM) is a devastating infectious neurological disease and the proteins and pathways involved in its pathophysiology are not fully understood. MATERIALS AND METHODS: Label-free quantitative (LFQ) proteomics analyses was used to identify differentially...

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Autores principales: Chen, Juncao, Huang, Weiben, Zhang, Hong, Peng, Xiangwen, Yang, Jun, Yang, Yong, Su, Jinzhen, Wang, Siyao, Zhou, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9424622/
https://www.ncbi.nlm.nih.gov/pubmed/36052359
http://dx.doi.org/10.3389/fped.2022.972032
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author Chen, Juncao
Huang, Weiben
Zhang, Hong
Peng, Xiangwen
Yang, Jun
Yang, Yong
Su, Jinzhen
Wang, Siyao
Zhou, Wei
author_facet Chen, Juncao
Huang, Weiben
Zhang, Hong
Peng, Xiangwen
Yang, Jun
Yang, Yong
Su, Jinzhen
Wang, Siyao
Zhou, Wei
author_sort Chen, Juncao
collection PubMed
description OBJECTIVE: Hydrocephalus in bacterial meningitis (BM) is a devastating infectious neurological disease and the proteins and pathways involved in its pathophysiology are not fully understood. MATERIALS AND METHODS: Label-free quantitative (LFQ) proteomics analyses was used to identify differentially expressed proteins (DEPs) in cerebrospinal fluid (CSF) samples from infants with hydrocephalus and bacterial meningitis (HBM group, N = 8), infants with bacterial meningitis (BM group, N = 9); and healthy infants (N group, N = 11). Bioinformatics analysis was subsequently performed to investigate Gene Ontology (GO) functional annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) enriched signaling pathways of these DEPs. Six proteins (AZU1, COX4I1, EDF1, KRT31, MMP12, and PRG2) were selected for further validation via enzyme-linked immunosorbent assay (ELISA). RESULTS: Compared with BM group and N group, HBM group had a higher whole CSF protein level (5.6 ± 2.7 vs. 1.7 ± 1.0 vs. 1.2 ± 0.5 g/l) and lower whole CSF glucose level (0.8 ± 0.6 vs. 1.8 ± 0.7 vs. 3.3 ± 0.8 mmol/l) (both P < 0.05). Over 300 DEPs were differentially expressed in HBM group compared with BM group and BM compared with N group, of which 78% were common to both. Cluster analysis indicated that the levels of 226 proteins were increased in BM group compared with N group and were decreased in HBM group compared with BM group. Bioinformatics analysis indicated the involvement of the cell adhesion, immune response and extracellular exosome signaling were significantly enriched in HBM compared with BM group and BM compared with N group. 267 DEPs were identified between HBM group with N group, KEGG analysis indicated that DEPs mainly involved in filament cytoskeleton and immune response. The ELISA results further verified that the expression levels of AZU1 were significantly different from among three groups (both P < 0.05). CONCLUSION: This is the first reported characterization of quantitative proteomics from the CSF of infants with HBM. Our study also demonstrated that AZU1 could be a potential biomarker for the diagnosis of hydrocephalus in bacterial meningitis.
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spelling pubmed-94246222022-08-31 Quantitative proteomics on the cerebrospinal fluid of hydrocephalus in neonatal bacterial meningitis Chen, Juncao Huang, Weiben Zhang, Hong Peng, Xiangwen Yang, Jun Yang, Yong Su, Jinzhen Wang, Siyao Zhou, Wei Front Pediatr Pediatrics OBJECTIVE: Hydrocephalus in bacterial meningitis (BM) is a devastating infectious neurological disease and the proteins and pathways involved in its pathophysiology are not fully understood. MATERIALS AND METHODS: Label-free quantitative (LFQ) proteomics analyses was used to identify differentially expressed proteins (DEPs) in cerebrospinal fluid (CSF) samples from infants with hydrocephalus and bacterial meningitis (HBM group, N = 8), infants with bacterial meningitis (BM group, N = 9); and healthy infants (N group, N = 11). Bioinformatics analysis was subsequently performed to investigate Gene Ontology (GO) functional annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) enriched signaling pathways of these DEPs. Six proteins (AZU1, COX4I1, EDF1, KRT31, MMP12, and PRG2) were selected for further validation via enzyme-linked immunosorbent assay (ELISA). RESULTS: Compared with BM group and N group, HBM group had a higher whole CSF protein level (5.6 ± 2.7 vs. 1.7 ± 1.0 vs. 1.2 ± 0.5 g/l) and lower whole CSF glucose level (0.8 ± 0.6 vs. 1.8 ± 0.7 vs. 3.3 ± 0.8 mmol/l) (both P < 0.05). Over 300 DEPs were differentially expressed in HBM group compared with BM group and BM compared with N group, of which 78% were common to both. Cluster analysis indicated that the levels of 226 proteins were increased in BM group compared with N group and were decreased in HBM group compared with BM group. Bioinformatics analysis indicated the involvement of the cell adhesion, immune response and extracellular exosome signaling were significantly enriched in HBM compared with BM group and BM compared with N group. 267 DEPs were identified between HBM group with N group, KEGG analysis indicated that DEPs mainly involved in filament cytoskeleton and immune response. The ELISA results further verified that the expression levels of AZU1 were significantly different from among three groups (both P < 0.05). CONCLUSION: This is the first reported characterization of quantitative proteomics from the CSF of infants with HBM. Our study also demonstrated that AZU1 could be a potential biomarker for the diagnosis of hydrocephalus in bacterial meningitis. Frontiers Media S.A. 2022-08-16 /pmc/articles/PMC9424622/ /pubmed/36052359 http://dx.doi.org/10.3389/fped.2022.972032 Text en Copyright © 2022 Chen, Huang, Zhang, Peng, Yang, Yang, Su, Wang and Zhou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pediatrics
Chen, Juncao
Huang, Weiben
Zhang, Hong
Peng, Xiangwen
Yang, Jun
Yang, Yong
Su, Jinzhen
Wang, Siyao
Zhou, Wei
Quantitative proteomics on the cerebrospinal fluid of hydrocephalus in neonatal bacterial meningitis
title Quantitative proteomics on the cerebrospinal fluid of hydrocephalus in neonatal bacterial meningitis
title_full Quantitative proteomics on the cerebrospinal fluid of hydrocephalus in neonatal bacterial meningitis
title_fullStr Quantitative proteomics on the cerebrospinal fluid of hydrocephalus in neonatal bacterial meningitis
title_full_unstemmed Quantitative proteomics on the cerebrospinal fluid of hydrocephalus in neonatal bacterial meningitis
title_short Quantitative proteomics on the cerebrospinal fluid of hydrocephalus in neonatal bacterial meningitis
title_sort quantitative proteomics on the cerebrospinal fluid of hydrocephalus in neonatal bacterial meningitis
topic Pediatrics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9424622/
https://www.ncbi.nlm.nih.gov/pubmed/36052359
http://dx.doi.org/10.3389/fped.2022.972032
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