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Mass cytometry immunostaining protocol for multiplexing clinical samples

This is a cytometry by time-of-flight (CyTOF) staining protocol for hematopoietic-derived cells, that leverages live-cell barcoding using receptor-type tyrosine-protein phosphatase C (CD45) antibodies conjugated to metal isotopes in combination with DNA-based palladium barcoding to multiplex up to 4...

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Detalles Bibliográficos
Autores principales: Gadalla, Ramy, Boukhaled, Giselle M., Brooks, David G., Wang, Ben X.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9424627/
https://www.ncbi.nlm.nih.gov/pubmed/36052346
http://dx.doi.org/10.1016/j.xpro.2022.101643
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author Gadalla, Ramy
Boukhaled, Giselle M.
Brooks, David G.
Wang, Ben X.
author_facet Gadalla, Ramy
Boukhaled, Giselle M.
Brooks, David G.
Wang, Ben X.
author_sort Gadalla, Ramy
collection PubMed
description This is a cytometry by time-of-flight (CyTOF) staining protocol for hematopoietic-derived cells, that leverages live-cell barcoding using receptor-type tyrosine-protein phosphatase C (CD45) antibodies conjugated to metal isotopes in combination with DNA-based palladium barcoding to multiplex up to 40 samples. In this protocol, DNA-based barcoding is performed before surface and intracellular immunostaining, which reduces the batch effects that result from day-to-day variations in staining and instrument sensitivity. This protocol also reduces antibody consumption and eliminates the need for repeated instrument adjustment.
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spelling pubmed-94246272022-08-31 Mass cytometry immunostaining protocol for multiplexing clinical samples Gadalla, Ramy Boukhaled, Giselle M. Brooks, David G. Wang, Ben X. STAR Protoc Protocol This is a cytometry by time-of-flight (CyTOF) staining protocol for hematopoietic-derived cells, that leverages live-cell barcoding using receptor-type tyrosine-protein phosphatase C (CD45) antibodies conjugated to metal isotopes in combination with DNA-based palladium barcoding to multiplex up to 40 samples. In this protocol, DNA-based barcoding is performed before surface and intracellular immunostaining, which reduces the batch effects that result from day-to-day variations in staining and instrument sensitivity. This protocol also reduces antibody consumption and eliminates the need for repeated instrument adjustment. Elsevier 2022-08-22 /pmc/articles/PMC9424627/ /pubmed/36052346 http://dx.doi.org/10.1016/j.xpro.2022.101643 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Gadalla, Ramy
Boukhaled, Giselle M.
Brooks, David G.
Wang, Ben X.
Mass cytometry immunostaining protocol for multiplexing clinical samples
title Mass cytometry immunostaining protocol for multiplexing clinical samples
title_full Mass cytometry immunostaining protocol for multiplexing clinical samples
title_fullStr Mass cytometry immunostaining protocol for multiplexing clinical samples
title_full_unstemmed Mass cytometry immunostaining protocol for multiplexing clinical samples
title_short Mass cytometry immunostaining protocol for multiplexing clinical samples
title_sort mass cytometry immunostaining protocol for multiplexing clinical samples
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9424627/
https://www.ncbi.nlm.nih.gov/pubmed/36052346
http://dx.doi.org/10.1016/j.xpro.2022.101643
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