Cargando…

Mass cytometry immunostaining protocol for multiplexing clinical samples

This is a cytometry by time-of-flight (CyTOF) staining protocol for hematopoietic-derived cells, that leverages live-cell barcoding using receptor-type tyrosine-protein phosphatase C (CD45) antibodies conjugated to metal isotopes in combination with DNA-based palladium barcoding to multiplex up to 4...

Descripción completa

Detalles Bibliográficos
Autores principales:	Gadalla, Ramy, Boukhaled, Giselle M., Brooks, David G., Wang, Ben X.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9424627/ https://www.ncbi.nlm.nih.gov/pubmed/36052346 http://dx.doi.org/10.1016/j.xpro.2022.101643

Ejemplares similares

Multiplex Cell Fate Tracking by Flow Cytometry
por: Rodríguez-Martínez, Marta, et al.
Publicado: (2020)

High-Throughput Mass Cytometry Staining for Immunophenotyping Clinical Samples
por: Thrash, Emily M., et al.
Publicado: (2020)

Mass cytometry staining for human bone marrow clinical samples
por: Hallisey, Margaret, et al.
Publicado: (2022)

Simplified mass cytometry protocol for in-plate staining, barcoding, and cryopreservation of human PBMC samples in clinical trials
por: Abrecht, Charlotte, et al.
Publicado: (2022)

An optimized protocol for phenotyping human granulocytes by mass cytometry
por: Vivanco Gonzalez, Nora, et al.
Publicado: (2022)

ChipCytometry for multiplexed detection of protein and mRNA markers on human FFPE tissue samples
por: Jarosch, Sebastian, et al.
Publicado: (2022)

Intranuclear immunostaining-based FACS protocol from embryonic cortical tissue
por: Sakib, M. Sadman, et al.
Publicado: (2021)

Protocol for whole-brain immunostaining of the turquoise killifish after tissue clearing
por: Lee, Seongsin, et al.
Publicado: (2021)

Flow-cytometry-based protocols for human blood/marrow immunophenotyping with minimal sample perturbation
por: Rico, Laura G., et al.
Publicado: (2021)

Immunostaining protocol for infiltrating brain cancer spheroids for light-sheet imaging
por: Bjørknes, Benedicte, et al.
Publicado: (2023)

Protocol using lentivirus to establish THP-1 suspension cell lines for immunostaining and confocal microscopy
por: Ji, Wangsheng, et al.
Publicado: (2023)

Protocol for large scale whole blood immune monitoring by mass cytometry and Cyto Quality Pipeline
por: Rybakowska, Paulina, et al.
Publicado: (2022)

ACE2 chromogenic immunostaining protocol optimized for formalin-fixed paraffin-embedded human tissue sections
por: Jorgensen, Marda, et al.
Publicado: (2021)

Isolation, culture, and immunostaining of neonatal rat ventricular myocytes
por: Pereira, Ana Helena Macedo, et al.
Publicado: (2021)

Immunostaining of intact C. elegans using polyacrylamide embedding
por: Ghaddar, Abbas, et al.
Publicado: (2023)

MATISSE: An analysis protocol for combining imaging mass cytometry with fluorescence microscopy to generate single-cell data
por: Krijgsman, Daniëlle, et al.
Publicado: (2021)

Murine brain tumor microenvironment immunophenotyping using mass cytometry
por: McClellan, Brandon L., et al.
Publicado: (2022)

Protocol for generating a mouse model of gastric MALT lymphoma and the identification of MALT lymphoma cell populations by immunostaining
por: Yamamoto, Koji, et al.
Publicado: (2022)

Protocol for multimodal analysis of human kidney tissue by imaging mass spectrometry and CODEX multiplexed immunofluorescence
por: Neumann, Elizabeth K., et al.
Publicado: (2021)

Protocol for analysis of mouse neutrophil NETosis by flow cytometry
por: McGill, Cassandra J., et al.
Publicado: (2021)

Visualization of cortical microtubule networks in plant cells by live imaging and immunostaining
por: Du, Fei, et al.
Publicado: (2021)

Dissections of Larval, Pupal and Adult Butterfly Brains for Immunostaining and Molecular Analysis
por: Toh, Yi Peng, et al.
Publicado: (2021)

Detection of RNA-DNA hybrids by immunostaining in meiotic nuclei of Saccharomyces cerevisiae
por: Yang, Xuan, et al.
Publicado: (2022)

High-throughput mass cytometry staining for deep phenotyping of human natural killer cells
por: Ben Amara, Amira, et al.
Publicado: (2022)

A flow-cytometry-based protocol using diverse cell types for detecting autoantibodies from human plasma and serum samples
por: Wong, Andrew Kam Ho, et al.
Publicado: (2021)

Characterization of macrophage infiltration and polarization by double fluorescence immunostaining in mouse colonic mucosa
por: Chiloeches, María López, et al.
Publicado: (2021)

Quantifying spatial position in a branched structure in immunostained mouse tissue sections
por: Heilmann, Silja, et al.
Publicado: (2021)

Rapid monoisotopic cisplatin based barcoding for multiplexed mass cytometry
por: McCarthy, Ryan L., et al.
Publicado: (2017)

Extended live-cell barcoding approach for multiplexed mass cytometry
por: Muftuoglu, Muharrem, et al.
Publicado: (2021)

Functional analysis of human circulating immune cells based on high-dimensional mass cytometry
por: Liu, Xiuxing, et al.
Publicado: (2022)

Isolation, cultivation and immunostaining of single myofibers: An improved approach to study the behavior of satellite cells
por: Lim, Chai Ling, et al.
Publicado: (2018)

Using pERK immunostaining to quantify neuronal activity induced by stress in zebrafish larvae
por: Corradi, Laura, et al.
Publicado: (2022)

Protocol for simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry
por: Daumoine, Susy, et al.
Publicado: (2023)

Multiplex Staining by Sequential Immunostaining and Antibody Removal on Routine Tissue Sections
por: Bolognesi, Maddalena Maria, et al.
Publicado: (2017)

Simultaneous Multiplexed Imaging of mRNA and Proteins with Subcellular Resolution in Breast Cancer Tissue Samples by Mass Cytometry
por: Schulz, Daniel, et al.
Publicado: (2018)

Simultaneous Multiplexed Imaging of mRNA and Proteins with Subcellular Resolution in Breast Cancer Tissue Samples by Mass Cytometry
por: Schulz, Daniel, et al.
Publicado: (2018)

High-dimensional functional phenotyping of preclinical human CAR T cells using mass cytometry
por: Michelozzi, Ilaria M., et al.
Publicado: (2022)

scQUEST: Quantifying tumor ecosystem heterogeneity from mass or flow cytometry data
por: Martinelli, Adriano Luca, et al.
Publicado: (2022)

Protocol for Analyzing Cell Cycle Phases of Polyphenic Wings Using Flow Cytometry
por: Zhang, Yuwei, et al.
Publicado: (2020)

Standardized flow-cytometry-based protocol to simultaneously measure transcription factor levels
por: Manso, Bryce A., et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_cdsrj48gcnn526qbkea63ovre5