Protocol for quantitative ethology on natural social interactions in Drosophila

Quantitative ethology of social interactions between free-moving animals enables precise measurement of behavioral kinematics critical for various disciplines such as neuroscience. Here, we describe a set of tools for quantitative ethology of social interactions including the analysis pipeline SoAL,...

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Autores principales: Ning, Jing, Li, Zhou, Zhang, Xingjiang, Wang, Junlong, Chen, Dandan, Liu, Qiong, Shao, Yefeng, Sun, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9424634/
https://www.ncbi.nlm.nih.gov/pubmed/36052344
http://dx.doi.org/10.1016/j.xpro.2022.101621
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author Ning, Jing
Li, Zhou
Zhang, Xingjiang
Wang, Junlong
Chen, Dandan
Liu, Qiong
Shao, Yefeng
Sun, Yi
author_facet Ning, Jing
Li, Zhou
Zhang, Xingjiang
Wang, Junlong
Chen, Dandan
Liu, Qiong
Shao, Yefeng
Sun, Yi
author_sort Ning, Jing
collection PubMed
description Quantitative ethology of social interactions between free-moving animals enables precise measurement of behavioral kinematics critical for various disciplines such as neuroscience. Here, we describe a set of tools for quantitative ethology of social interactions including the analysis pipeline SoAL, the training dataset SDPD, and the camera control software MIAS, along with experimental details. These tools are directly applicable for courtship behavior in Drosophila and can be used for other social interactions in other species after modification. For complete details on the use and execution of this protocol, please refer to Ning et al. (2022).
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spelling pubmed-94246342022-08-31 Protocol for quantitative ethology on natural social interactions in Drosophila Ning, Jing Li, Zhou Zhang, Xingjiang Wang, Junlong Chen, Dandan Liu, Qiong Shao, Yefeng Sun, Yi STAR Protoc Protocol Quantitative ethology of social interactions between free-moving animals enables precise measurement of behavioral kinematics critical for various disciplines such as neuroscience. Here, we describe a set of tools for quantitative ethology of social interactions including the analysis pipeline SoAL, the training dataset SDPD, and the camera control software MIAS, along with experimental details. These tools are directly applicable for courtship behavior in Drosophila and can be used for other social interactions in other species after modification. For complete details on the use and execution of this protocol, please refer to Ning et al. (2022). Elsevier 2022-08-23 /pmc/articles/PMC9424634/ /pubmed/36052344 http://dx.doi.org/10.1016/j.xpro.2022.101621 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ning, Jing
Li, Zhou
Zhang, Xingjiang
Wang, Junlong
Chen, Dandan
Liu, Qiong
Shao, Yefeng
Sun, Yi
Protocol for quantitative ethology on natural social interactions in Drosophila
title Protocol for quantitative ethology on natural social interactions in Drosophila
title_full Protocol for quantitative ethology on natural social interactions in Drosophila
title_fullStr Protocol for quantitative ethology on natural social interactions in Drosophila
title_full_unstemmed Protocol for quantitative ethology on natural social interactions in Drosophila
title_short Protocol for quantitative ethology on natural social interactions in Drosophila
title_sort protocol for quantitative ethology on natural social interactions in drosophila
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9424634/
https://www.ncbi.nlm.nih.gov/pubmed/36052344
http://dx.doi.org/10.1016/j.xpro.2022.101621
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