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Magnetic bead-based adsorption strategy for exosome isolation
Exosomes, one type of extracellular vesicle (EV) secreted by cells, participate in intercellular communication and other biological processes as carriers of lipids, functional proteins, mRNAs, miRNAs, lncRNAs, and DNA fragments. Their presence in biofluids makes them attractive candidates as innovat...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9424818/ https://www.ncbi.nlm.nih.gov/pubmed/36051582 http://dx.doi.org/10.3389/fbioe.2022.942077 |
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author | Jiawei, Sun Zhi, Chen Kewei, Tian Xiaoping, Li |
author_facet | Jiawei, Sun Zhi, Chen Kewei, Tian Xiaoping, Li |
author_sort | Jiawei, Sun |
collection | PubMed |
description | Exosomes, one type of extracellular vesicle (EV) secreted by cells, participate in intercellular communication and other biological processes as carriers of lipids, functional proteins, mRNAs, miRNAs, lncRNAs, and DNA fragments. Their presence in biofluids makes them attractive candidates as innovative clinical diagnostic tools. However, the conventional isolation and analysis of high-purity exosomes in clinical application is challenging, with traditional methods facing a number of shortcomings, including low yield or purity, long periods of processing, high cost, and difficulties in standardization. In this study, we provide an overview of commonly used exosome isolation approaches with a focus on magnetic bead-based capture, an ideal methodology with high purity and integrality of exosomes. The current challenges on exosome isolation methods are also described to highlight areas for future research and development. |
format | Online Article Text |
id | pubmed-9424818 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94248182022-08-31 Magnetic bead-based adsorption strategy for exosome isolation Jiawei, Sun Zhi, Chen Kewei, Tian Xiaoping, Li Front Bioeng Biotechnol Bioengineering and Biotechnology Exosomes, one type of extracellular vesicle (EV) secreted by cells, participate in intercellular communication and other biological processes as carriers of lipids, functional proteins, mRNAs, miRNAs, lncRNAs, and DNA fragments. Their presence in biofluids makes them attractive candidates as innovative clinical diagnostic tools. However, the conventional isolation and analysis of high-purity exosomes in clinical application is challenging, with traditional methods facing a number of shortcomings, including low yield or purity, long periods of processing, high cost, and difficulties in standardization. In this study, we provide an overview of commonly used exosome isolation approaches with a focus on magnetic bead-based capture, an ideal methodology with high purity and integrality of exosomes. The current challenges on exosome isolation methods are also described to highlight areas for future research and development. Frontiers Media S.A. 2022-08-16 /pmc/articles/PMC9424818/ /pubmed/36051582 http://dx.doi.org/10.3389/fbioe.2022.942077 Text en Copyright © 2022 Jiawei, Zhi, Kewei and Xiaoping. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Jiawei, Sun Zhi, Chen Kewei, Tian Xiaoping, Li Magnetic bead-based adsorption strategy for exosome isolation |
title | Magnetic bead-based adsorption strategy for exosome isolation |
title_full | Magnetic bead-based adsorption strategy for exosome isolation |
title_fullStr | Magnetic bead-based adsorption strategy for exosome isolation |
title_full_unstemmed | Magnetic bead-based adsorption strategy for exosome isolation |
title_short | Magnetic bead-based adsorption strategy for exosome isolation |
title_sort | magnetic bead-based adsorption strategy for exosome isolation |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9424818/ https://www.ncbi.nlm.nih.gov/pubmed/36051582 http://dx.doi.org/10.3389/fbioe.2022.942077 |
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