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Whole-genome sequence of a high-temperature edible mushroom Pleurotus giganteus (zhudugu)

Most of the sequenced wood-rotting edible mushroom produce fruiting body at relatively low temperatures. Little information has been known about the high-temperature wood-rotting mushroom. Here, we performed de novo sequencing and assembly of the genome of a high-temperature edible mushroom Pleurotu...

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Autores principales: Yu, Hailong, Zhang, Meiyan, Sun, Yating, Li, Qiaozhen, Liu, Jianyu, Song, Chunyan, Shang, Xiaodong, Tan, Qi, Zhang, Lujun, Yu, Hao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9424821/
https://www.ncbi.nlm.nih.gov/pubmed/36051764
http://dx.doi.org/10.3389/fmicb.2022.941889
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author Yu, Hailong
Zhang, Meiyan
Sun, Yating
Li, Qiaozhen
Liu, Jianyu
Song, Chunyan
Shang, Xiaodong
Tan, Qi
Zhang, Lujun
Yu, Hao
author_facet Yu, Hailong
Zhang, Meiyan
Sun, Yating
Li, Qiaozhen
Liu, Jianyu
Song, Chunyan
Shang, Xiaodong
Tan, Qi
Zhang, Lujun
Yu, Hao
author_sort Yu, Hailong
collection PubMed
description Most of the sequenced wood-rotting edible mushroom produce fruiting body at relatively low temperatures. Little information has been known about the high-temperature wood-rotting mushroom. Here, we performed de novo sequencing and assembly of the genome of a high-temperature edible mushroom Pleurotus giganteus from a monokaryotic strain zhudugu2 using the Illumina and Pac-Bio CLR sequencing technologies. P. giganteus, also known as Zhudugu in China, is a well-known culinary edible mushroom that has been widely distributed and cultivated in China, Southeast Asia, and South Asia. The genome consists of 40.00 Mb in 27 contigs with a contig N50 of 4.384 Mb. Phylogenetic analysis reveals that P. giganteus and other strains in Pleurotus clustered in one clade. Phylogenetic analysis and average nucleotide identity analysis indicated that the P. giganteus genome showed a closer relationship with other Pleurotus species. Chromosome collinearity analysis revealed a high level of collinearity between P. ostreatus and P. giganteus. There are 12,628 protein-coding genes annotated in this monoploid genome. A total of 481 enzymes accounting for 514 carbohydrate-active enzymes (CAZymes) terms were identified in the P. giganteus genome, including 15 laccases and 10 class II peroxidases predicted in the genome, which revealed the robustness of lignocellulose degradation capacity of P. giganteus. The mating-A type locus of P. giganteus consisted of a pair of homeodomain mating-type genes HD1 and HD2. The mating-B type locus of P. giganteus consisted of at least four pheromone receptor genes and three pheromone genes. The genome is not only beneficial for the genome-assisted breeding of this mushroom but also helps us to understand the high-temperature tolerance of the edible mushroom.
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spelling pubmed-94248212022-08-31 Whole-genome sequence of a high-temperature edible mushroom Pleurotus giganteus (zhudugu) Yu, Hailong Zhang, Meiyan Sun, Yating Li, Qiaozhen Liu, Jianyu Song, Chunyan Shang, Xiaodong Tan, Qi Zhang, Lujun Yu, Hao Front Microbiol Microbiology Most of the sequenced wood-rotting edible mushroom produce fruiting body at relatively low temperatures. Little information has been known about the high-temperature wood-rotting mushroom. Here, we performed de novo sequencing and assembly of the genome of a high-temperature edible mushroom Pleurotus giganteus from a monokaryotic strain zhudugu2 using the Illumina and Pac-Bio CLR sequencing technologies. P. giganteus, also known as Zhudugu in China, is a well-known culinary edible mushroom that has been widely distributed and cultivated in China, Southeast Asia, and South Asia. The genome consists of 40.00 Mb in 27 contigs with a contig N50 of 4.384 Mb. Phylogenetic analysis reveals that P. giganteus and other strains in Pleurotus clustered in one clade. Phylogenetic analysis and average nucleotide identity analysis indicated that the P. giganteus genome showed a closer relationship with other Pleurotus species. Chromosome collinearity analysis revealed a high level of collinearity between P. ostreatus and P. giganteus. There are 12,628 protein-coding genes annotated in this monoploid genome. A total of 481 enzymes accounting for 514 carbohydrate-active enzymes (CAZymes) terms were identified in the P. giganteus genome, including 15 laccases and 10 class II peroxidases predicted in the genome, which revealed the robustness of lignocellulose degradation capacity of P. giganteus. The mating-A type locus of P. giganteus consisted of a pair of homeodomain mating-type genes HD1 and HD2. The mating-B type locus of P. giganteus consisted of at least four pheromone receptor genes and three pheromone genes. The genome is not only beneficial for the genome-assisted breeding of this mushroom but also helps us to understand the high-temperature tolerance of the edible mushroom. Frontiers Media S.A. 2022-08-16 /pmc/articles/PMC9424821/ /pubmed/36051764 http://dx.doi.org/10.3389/fmicb.2022.941889 Text en Copyright © 2022 Yu, Zhang, Sun, Li, Liu, Song, Shang, Tan, Zhang and Yu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Yu, Hailong
Zhang, Meiyan
Sun, Yating
Li, Qiaozhen
Liu, Jianyu
Song, Chunyan
Shang, Xiaodong
Tan, Qi
Zhang, Lujun
Yu, Hao
Whole-genome sequence of a high-temperature edible mushroom Pleurotus giganteus (zhudugu)
title Whole-genome sequence of a high-temperature edible mushroom Pleurotus giganteus (zhudugu)
title_full Whole-genome sequence of a high-temperature edible mushroom Pleurotus giganteus (zhudugu)
title_fullStr Whole-genome sequence of a high-temperature edible mushroom Pleurotus giganteus (zhudugu)
title_full_unstemmed Whole-genome sequence of a high-temperature edible mushroom Pleurotus giganteus (zhudugu)
title_short Whole-genome sequence of a high-temperature edible mushroom Pleurotus giganteus (zhudugu)
title_sort whole-genome sequence of a high-temperature edible mushroom pleurotus giganteus (zhudugu)
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9424821/
https://www.ncbi.nlm.nih.gov/pubmed/36051764
http://dx.doi.org/10.3389/fmicb.2022.941889
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