Fungal ablation and transplantation of specific fungal species into PDAC tumor-bearing mice

Intratumor and gut mycobiome is linked to pancreatic ductal adenocarcinoma (PDAC) tumorigenesis; however, an optimal approach to culture and transplant fungus into mouse for in vivo studies is missing. This protocol describes culture steps of Alternaria alternata and Malassezia globosa and their sub...

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Autores principales: Alam, Aftab, Comer, Sarah, Levanduski, Eric, Dey, Prasenjit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9425075/
https://www.ncbi.nlm.nih.gov/pubmed/36052343
http://dx.doi.org/10.1016/j.xpro.2022.101644
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author Alam, Aftab
Comer, Sarah
Levanduski, Eric
Dey, Prasenjit
author_facet Alam, Aftab
Comer, Sarah
Levanduski, Eric
Dey, Prasenjit
author_sort Alam, Aftab
collection PubMed
description Intratumor and gut mycobiome is linked to pancreatic ductal adenocarcinoma (PDAC) tumorigenesis; however, an optimal approach to culture and transplant fungus into mouse for in vivo studies is missing. This protocol describes culture steps of Alternaria alternata and Malassezia globosa and their subsequent transplantation into a PDAC mouse model via oral gavage. The utilization of the fungal culture method will allow for consistent growth and expansion of specific fungal species for downstream processing. For complete details on the use and execution of this protocol, please refer to Alam et al. (2022).
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spelling pubmed-94250752022-08-31 Fungal ablation and transplantation of specific fungal species into PDAC tumor-bearing mice Alam, Aftab Comer, Sarah Levanduski, Eric Dey, Prasenjit STAR Protoc Protocol Intratumor and gut mycobiome is linked to pancreatic ductal adenocarcinoma (PDAC) tumorigenesis; however, an optimal approach to culture and transplant fungus into mouse for in vivo studies is missing. This protocol describes culture steps of Alternaria alternata and Malassezia globosa and their subsequent transplantation into a PDAC mouse model via oral gavage. The utilization of the fungal culture method will allow for consistent growth and expansion of specific fungal species for downstream processing. For complete details on the use and execution of this protocol, please refer to Alam et al. (2022). Elsevier 2022-08-23 /pmc/articles/PMC9425075/ /pubmed/36052343 http://dx.doi.org/10.1016/j.xpro.2022.101644 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Alam, Aftab
Comer, Sarah
Levanduski, Eric
Dey, Prasenjit
Fungal ablation and transplantation of specific fungal species into PDAC tumor-bearing mice
title Fungal ablation and transplantation of specific fungal species into PDAC tumor-bearing mice
title_full Fungal ablation and transplantation of specific fungal species into PDAC tumor-bearing mice
title_fullStr Fungal ablation and transplantation of specific fungal species into PDAC tumor-bearing mice
title_full_unstemmed Fungal ablation and transplantation of specific fungal species into PDAC tumor-bearing mice
title_short Fungal ablation and transplantation of specific fungal species into PDAC tumor-bearing mice
title_sort fungal ablation and transplantation of specific fungal species into pdac tumor-bearing mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9425075/
https://www.ncbi.nlm.nih.gov/pubmed/36052343
http://dx.doi.org/10.1016/j.xpro.2022.101644
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AT deyprasenjit fungalablationandtransplantationofspecificfungalspeciesintopdactumorbearingmice

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