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Development of sandwich-form biosensor to detect Mycobacterium tuberculosis complex in clinical sputum specimens

Mycobacterium tuberculosis, the causing agent of tuberculosis, comes second only after HIV on the list of infectious agents slaughtering many worldwide. Due to the limitations behind the conventional detection methods, it is therefore critical to develop new sensitive sensing systems capable of quic...

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Autores principales: Shojaei, Taha Roodbar, Mohd Salleh, Mohamad Amran, Tabatabaei, Meisam, Ekrami, Alireza, Motallebi, Roya, Rahmani-Cherati, Tavoos, Hajalilou, Abdollah, Jorfi, Raheleh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9425227/
https://www.ncbi.nlm.nih.gov/pubmed/25181404
http://dx.doi.org/10.1016/j.bjid.2014.05.015
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author Shojaei, Taha Roodbar
Mohd Salleh, Mohamad Amran
Tabatabaei, Meisam
Ekrami, Alireza
Motallebi, Roya
Rahmani-Cherati, Tavoos
Hajalilou, Abdollah
Jorfi, Raheleh
author_facet Shojaei, Taha Roodbar
Mohd Salleh, Mohamad Amran
Tabatabaei, Meisam
Ekrami, Alireza
Motallebi, Roya
Rahmani-Cherati, Tavoos
Hajalilou, Abdollah
Jorfi, Raheleh
author_sort Shojaei, Taha Roodbar
collection PubMed
description Mycobacterium tuberculosis, the causing agent of tuberculosis, comes second only after HIV on the list of infectious agents slaughtering many worldwide. Due to the limitations behind the conventional detection methods, it is therefore critical to develop new sensitive sensing systems capable of quick detection of the infectious agent. In the present study, the surface modified cadmium-telluride quantum dots and gold nanoparticles conjunct with two specific oligonucleotides against early secretory antigenic target 6 were used to develop a sandwich-form fluorescence resonance energy transfer-based biosensor to detect M. tuberculosis complex and differentiate M. tuberculosis and M. bovis Bacille Calmette–Guerin simultaneously. The sensitivity and specificity of the newly developed biosensor were 94.2% and 86.6%, respectively, while the sensitivity and specificity of polymerase chain reaction and nested polymerase chain reaction were considerably lower, 74.2%, 73.3% and 82.8%, 80%, respectively. The detection limits of the sandwich-form fluorescence resonance energy transfer-based biosensor were far lower (10 fg) than those of the polymerase chain reaction and nested polymerase chain reaction (100 fg). Although the cost of the developed nanobiosensor was slightly higher than those of the polymerase chain reaction-based techniques, its unique advantages in terms of turnaround time, higher sensitivity and specificity, as well as a 10-fold lower detection limit would clearly recommend this test as a more appropriate and cost-effective tool for large scale operations.
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spelling pubmed-94252272022-08-31 Development of sandwich-form biosensor to detect Mycobacterium tuberculosis complex in clinical sputum specimens Shojaei, Taha Roodbar Mohd Salleh, Mohamad Amran Tabatabaei, Meisam Ekrami, Alireza Motallebi, Roya Rahmani-Cherati, Tavoos Hajalilou, Abdollah Jorfi, Raheleh Braz J Infect Dis Original Article Mycobacterium tuberculosis, the causing agent of tuberculosis, comes second only after HIV on the list of infectious agents slaughtering many worldwide. Due to the limitations behind the conventional detection methods, it is therefore critical to develop new sensitive sensing systems capable of quick detection of the infectious agent. In the present study, the surface modified cadmium-telluride quantum dots and gold nanoparticles conjunct with two specific oligonucleotides against early secretory antigenic target 6 were used to develop a sandwich-form fluorescence resonance energy transfer-based biosensor to detect M. tuberculosis complex and differentiate M. tuberculosis and M. bovis Bacille Calmette–Guerin simultaneously. The sensitivity and specificity of the newly developed biosensor were 94.2% and 86.6%, respectively, while the sensitivity and specificity of polymerase chain reaction and nested polymerase chain reaction were considerably lower, 74.2%, 73.3% and 82.8%, 80%, respectively. The detection limits of the sandwich-form fluorescence resonance energy transfer-based biosensor were far lower (10 fg) than those of the polymerase chain reaction and nested polymerase chain reaction (100 fg). Although the cost of the developed nanobiosensor was slightly higher than those of the polymerase chain reaction-based techniques, its unique advantages in terms of turnaround time, higher sensitivity and specificity, as well as a 10-fold lower detection limit would clearly recommend this test as a more appropriate and cost-effective tool for large scale operations. Elsevier 2014-08-30 /pmc/articles/PMC9425227/ /pubmed/25181404 http://dx.doi.org/10.1016/j.bjid.2014.05.015 Text en © 2014 Elsevier Editora Ltda. Este é um artigo Open Access sob a licença de CC BY-NC-ND. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Shojaei, Taha Roodbar
Mohd Salleh, Mohamad Amran
Tabatabaei, Meisam
Ekrami, Alireza
Motallebi, Roya
Rahmani-Cherati, Tavoos
Hajalilou, Abdollah
Jorfi, Raheleh
Development of sandwich-form biosensor to detect Mycobacterium tuberculosis complex in clinical sputum specimens
title Development of sandwich-form biosensor to detect Mycobacterium tuberculosis complex in clinical sputum specimens
title_full Development of sandwich-form biosensor to detect Mycobacterium tuberculosis complex in clinical sputum specimens
title_fullStr Development of sandwich-form biosensor to detect Mycobacterium tuberculosis complex in clinical sputum specimens
title_full_unstemmed Development of sandwich-form biosensor to detect Mycobacterium tuberculosis complex in clinical sputum specimens
title_short Development of sandwich-form biosensor to detect Mycobacterium tuberculosis complex in clinical sputum specimens
title_sort development of sandwich-form biosensor to detect mycobacterium tuberculosis complex in clinical sputum specimens
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9425227/
https://www.ncbi.nlm.nih.gov/pubmed/25181404
http://dx.doi.org/10.1016/j.bjid.2014.05.015
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