Cargando…
Performance and validation of an adaptable multiplex assay for detection of serologic response to SARS-CoV-2 infection or vaccination
Measurement of quantitative antibody responses are increasingly important in evaluating the immune response to infection and vaccination. In this study we describe the validation of a quantitative, multiplex serologic assay utilising an electrochemiluminescence platform, which measures IgG against t...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Authors. Published by Elsevier B.V.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9425705/ https://www.ncbi.nlm.nih.gov/pubmed/36055441 http://dx.doi.org/10.1016/j.jim.2022.113345 |
_version_ | 1784778508070813696 |
---|---|
author | Kenny, Grace Negi, Riya O'Reilly, Sophie Garcia-Leon, Alejandro Alalwan, Dana Gaillard, Colette Marie Saini, Gurvin Inzitari, Rosana Feeney, Eoin R. Yousif, Obada Cotter, Aoife G de Barra, Eoghan Sadlier, Corinna Crispie, Fiona Doran, Peter Gautier, Virginie Mallon, Patrick W.G. |
author_facet | Kenny, Grace Negi, Riya O'Reilly, Sophie Garcia-Leon, Alejandro Alalwan, Dana Gaillard, Colette Marie Saini, Gurvin Inzitari, Rosana Feeney, Eoin R. Yousif, Obada Cotter, Aoife G de Barra, Eoghan Sadlier, Corinna Crispie, Fiona Doran, Peter Gautier, Virginie Mallon, Patrick W.G. |
author_sort | Kenny, Grace |
collection | PubMed |
description | Measurement of quantitative antibody responses are increasingly important in evaluating the immune response to infection and vaccination. In this study we describe the validation of a quantitative, multiplex serologic assay utilising an electrochemiluminescence platform, which measures IgG against the receptor binding domain (RBD), spike S1 and S2 subunits and nucleocapsid antigens of SARS-CoV-2. The assay displayed a sensitivity ranging from 73 to 91% and specificity from 90 to 96% in detecting previous infection with SARS-CoV-2 depending on antigenic target and time since infection, and this assay highly correlated with commercially available assays. The within-plate coefficient of variation ranged from 3.8–3.9% and the inter-plate coefficient of variation from 11 to 13% for each antigen. |
format | Online Article Text |
id | pubmed-9425705 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Authors. Published by Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94257052022-08-30 Performance and validation of an adaptable multiplex assay for detection of serologic response to SARS-CoV-2 infection or vaccination Kenny, Grace Negi, Riya O'Reilly, Sophie Garcia-Leon, Alejandro Alalwan, Dana Gaillard, Colette Marie Saini, Gurvin Inzitari, Rosana Feeney, Eoin R. Yousif, Obada Cotter, Aoife G de Barra, Eoghan Sadlier, Corinna Crispie, Fiona Doran, Peter Gautier, Virginie Mallon, Patrick W.G. J Immunol Methods Article Measurement of quantitative antibody responses are increasingly important in evaluating the immune response to infection and vaccination. In this study we describe the validation of a quantitative, multiplex serologic assay utilising an electrochemiluminescence platform, which measures IgG against the receptor binding domain (RBD), spike S1 and S2 subunits and nucleocapsid antigens of SARS-CoV-2. The assay displayed a sensitivity ranging from 73 to 91% and specificity from 90 to 96% in detecting previous infection with SARS-CoV-2 depending on antigenic target and time since infection, and this assay highly correlated with commercially available assays. The within-plate coefficient of variation ranged from 3.8–3.9% and the inter-plate coefficient of variation from 11 to 13% for each antigen. The Authors. Published by Elsevier B.V. 2022-11 2022-08-30 /pmc/articles/PMC9425705/ /pubmed/36055441 http://dx.doi.org/10.1016/j.jim.2022.113345 Text en © 2022 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Kenny, Grace Negi, Riya O'Reilly, Sophie Garcia-Leon, Alejandro Alalwan, Dana Gaillard, Colette Marie Saini, Gurvin Inzitari, Rosana Feeney, Eoin R. Yousif, Obada Cotter, Aoife G de Barra, Eoghan Sadlier, Corinna Crispie, Fiona Doran, Peter Gautier, Virginie Mallon, Patrick W.G. Performance and validation of an adaptable multiplex assay for detection of serologic response to SARS-CoV-2 infection or vaccination |
title | Performance and validation of an adaptable multiplex assay for detection of serologic response to SARS-CoV-2 infection or vaccination |
title_full | Performance and validation of an adaptable multiplex assay for detection of serologic response to SARS-CoV-2 infection or vaccination |
title_fullStr | Performance and validation of an adaptable multiplex assay for detection of serologic response to SARS-CoV-2 infection or vaccination |
title_full_unstemmed | Performance and validation of an adaptable multiplex assay for detection of serologic response to SARS-CoV-2 infection or vaccination |
title_short | Performance and validation of an adaptable multiplex assay for detection of serologic response to SARS-CoV-2 infection or vaccination |
title_sort | performance and validation of an adaptable multiplex assay for detection of serologic response to sars-cov-2 infection or vaccination |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9425705/ https://www.ncbi.nlm.nih.gov/pubmed/36055441 http://dx.doi.org/10.1016/j.jim.2022.113345 |
work_keys_str_mv | AT kennygrace performanceandvalidationofanadaptablemultiplexassayfordetectionofserologicresponsetosarscov2infectionorvaccination AT negiriya performanceandvalidationofanadaptablemultiplexassayfordetectionofserologicresponsetosarscov2infectionorvaccination AT oreillysophie performanceandvalidationofanadaptablemultiplexassayfordetectionofserologicresponsetosarscov2infectionorvaccination AT garcialeonalejandro performanceandvalidationofanadaptablemultiplexassayfordetectionofserologicresponsetosarscov2infectionorvaccination AT alalwandana performanceandvalidationofanadaptablemultiplexassayfordetectionofserologicresponsetosarscov2infectionorvaccination AT gaillardcolettemarie performanceandvalidationofanadaptablemultiplexassayfordetectionofserologicresponsetosarscov2infectionorvaccination AT sainigurvin performanceandvalidationofanadaptablemultiplexassayfordetectionofserologicresponsetosarscov2infectionorvaccination AT inzitarirosana performanceandvalidationofanadaptablemultiplexassayfordetectionofserologicresponsetosarscov2infectionorvaccination AT feeneyeoinr performanceandvalidationofanadaptablemultiplexassayfordetectionofserologicresponsetosarscov2infectionorvaccination AT yousifobada performanceandvalidationofanadaptablemultiplexassayfordetectionofserologicresponsetosarscov2infectionorvaccination AT cotteraoifeg performanceandvalidationofanadaptablemultiplexassayfordetectionofserologicresponsetosarscov2infectionorvaccination AT debarraeoghan performanceandvalidationofanadaptablemultiplexassayfordetectionofserologicresponsetosarscov2infectionorvaccination AT sadliercorinna performanceandvalidationofanadaptablemultiplexassayfordetectionofserologicresponsetosarscov2infectionorvaccination AT crispiefiona performanceandvalidationofanadaptablemultiplexassayfordetectionofserologicresponsetosarscov2infectionorvaccination AT doranpeter performanceandvalidationofanadaptablemultiplexassayfordetectionofserologicresponsetosarscov2infectionorvaccination AT gautiervirginie performanceandvalidationofanadaptablemultiplexassayfordetectionofserologicresponsetosarscov2infectionorvaccination AT mallonpatrickwg performanceandvalidationofanadaptablemultiplexassayfordetectionofserologicresponsetosarscov2infectionorvaccination AT performanceandvalidationofanadaptablemultiplexassayfordetectionofserologicresponsetosarscov2infectionorvaccination |