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HDA-2-Containing Complex Is Required for Activation of Catalase-3 Expression in Neurospora crassa
It is essential for aerobic organisms to maintain the homeostasis of intracellular reactive oxygen species (ROS) for survival and adaptation to the environment. In line with other eukaryotes, the catalase of Neurospora crassa is an important enzyme for clearing ROS, and its expression is tightly reg...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9426557/ https://www.ncbi.nlm.nih.gov/pubmed/35699373 http://dx.doi.org/10.1128/mbio.01351-22 |
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author | He, Lingaonan Duan, Zeyu Yu, Muqun Qi, Shaohua Wang, Ying Lou, Huiqiang He, Qun |
author_facet | He, Lingaonan Duan, Zeyu Yu, Muqun Qi, Shaohua Wang, Ying Lou, Huiqiang He, Qun |
author_sort | He, Lingaonan |
collection | PubMed |
description | It is essential for aerobic organisms to maintain the homeostasis of intracellular reactive oxygen species (ROS) for survival and adaptation to the environment. In line with other eukaryotes, the catalase of Neurospora crassa is an important enzyme for clearing ROS, and its expression is tightly regulated by the growth phase and various oxidative stresses. Our study reveals that, in N. crassa, histone deacetylase 2 (HDA-2) and its catalytic activity positively regulate the expression of the catalase-3 (cat-3) gene. HDA-2, SIF-2, and SNT-1 may form a subcomplex with such a regulation role. As expected, deletion of HDA-2 or SIF-2 subunit increased acetylation levels of histone H4, indicating that loss of HDA-2 complex fails to deacetylate H4 at the cat-3 locus. Furthermore, loss of HDA-2 or its catalytic activity led to dramatic decreases of TFIIB and RNA polymerase II (RNAP II) recruitment at the cat-3 locus and also resulted in high deposition of H2A.Z at the promoter and transcription start site (TSS) regions of the cat-3 gene. Collectively, this study strongly demonstrates that the HDA-2-containing complex activates the transcription of the cat-3 gene by facilitating preinitiation complex (PIC) assembly and antagonizing the inhibition of H2A.Z at the cat-3 locus through H4 acetylation. |
format | Online Article Text |
id | pubmed-9426557 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-94265572022-08-31 HDA-2-Containing Complex Is Required for Activation of Catalase-3 Expression in Neurospora crassa He, Lingaonan Duan, Zeyu Yu, Muqun Qi, Shaohua Wang, Ying Lou, Huiqiang He, Qun mBio Research Article It is essential for aerobic organisms to maintain the homeostasis of intracellular reactive oxygen species (ROS) for survival and adaptation to the environment. In line with other eukaryotes, the catalase of Neurospora crassa is an important enzyme for clearing ROS, and its expression is tightly regulated by the growth phase and various oxidative stresses. Our study reveals that, in N. crassa, histone deacetylase 2 (HDA-2) and its catalytic activity positively regulate the expression of the catalase-3 (cat-3) gene. HDA-2, SIF-2, and SNT-1 may form a subcomplex with such a regulation role. As expected, deletion of HDA-2 or SIF-2 subunit increased acetylation levels of histone H4, indicating that loss of HDA-2 complex fails to deacetylate H4 at the cat-3 locus. Furthermore, loss of HDA-2 or its catalytic activity led to dramatic decreases of TFIIB and RNA polymerase II (RNAP II) recruitment at the cat-3 locus and also resulted in high deposition of H2A.Z at the promoter and transcription start site (TSS) regions of the cat-3 gene. Collectively, this study strongly demonstrates that the HDA-2-containing complex activates the transcription of the cat-3 gene by facilitating preinitiation complex (PIC) assembly and antagonizing the inhibition of H2A.Z at the cat-3 locus through H4 acetylation. American Society for Microbiology 2022-06-14 /pmc/articles/PMC9426557/ /pubmed/35699373 http://dx.doi.org/10.1128/mbio.01351-22 Text en Copyright © 2022 He et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article He, Lingaonan Duan, Zeyu Yu, Muqun Qi, Shaohua Wang, Ying Lou, Huiqiang He, Qun HDA-2-Containing Complex Is Required for Activation of Catalase-3 Expression in Neurospora crassa |
title | HDA-2-Containing Complex Is Required for Activation of Catalase-3 Expression in Neurospora crassa |
title_full | HDA-2-Containing Complex Is Required for Activation of Catalase-3 Expression in Neurospora crassa |
title_fullStr | HDA-2-Containing Complex Is Required for Activation of Catalase-3 Expression in Neurospora crassa |
title_full_unstemmed | HDA-2-Containing Complex Is Required for Activation of Catalase-3 Expression in Neurospora crassa |
title_short | HDA-2-Containing Complex Is Required for Activation of Catalase-3 Expression in Neurospora crassa |
title_sort | hda-2-containing complex is required for activation of catalase-3 expression in neurospora crassa |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9426557/ https://www.ncbi.nlm.nih.gov/pubmed/35699373 http://dx.doi.org/10.1128/mbio.01351-22 |
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