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Microbiome Restructuring: Dominant Coral Bacterium Endozoicomonas Species Respond Differentially to Environmental Changes

Bacteria in the coral microbiome play a crucial role in determining coral health and fitness, and the coral host often restructures its microbiome composition in response to external factors. An important but often neglected factor determining this microbiome restructuring is the ability of microbio...

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Autores principales: Tandon, Kshitij, Chiou, Yu-Jing, Yu, Sheng-Ping, Hsieh, Hernyi Justin, Lu, Chih-Ying, Hsu, Ming-Tsung, Chiang, Pei-Wen, Chen, Hsing-Ju, Wada, Naohisa, Tang, Sen-Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9426584/
https://www.ncbi.nlm.nih.gov/pubmed/35703535
http://dx.doi.org/10.1128/msystems.00359-22
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author Tandon, Kshitij
Chiou, Yu-Jing
Yu, Sheng-Ping
Hsieh, Hernyi Justin
Lu, Chih-Ying
Hsu, Ming-Tsung
Chiang, Pei-Wen
Chen, Hsing-Ju
Wada, Naohisa
Tang, Sen-Lin
author_facet Tandon, Kshitij
Chiou, Yu-Jing
Yu, Sheng-Ping
Hsieh, Hernyi Justin
Lu, Chih-Ying
Hsu, Ming-Tsung
Chiang, Pei-Wen
Chen, Hsing-Ju
Wada, Naohisa
Tang, Sen-Lin
author_sort Tandon, Kshitij
collection PubMed
description Bacteria in the coral microbiome play a crucial role in determining coral health and fitness, and the coral host often restructures its microbiome composition in response to external factors. An important but often neglected factor determining this microbiome restructuring is the ability of microbiome members to respond to changes in the environment. To address this issue, we examined how the microbiome structure of Acropora muricata corals changed over 9 months following a reciprocal transplant experiment. Using a combination of metabarcoding, genomics, and comparative genomics approaches, we found that coral colonies separated by a small distance harbored different dominant Endozoicomonas-related phylotypes belonging to two different species, including a novel species, “Candidatus Endozoicomonas penghunesis” 4G, whose chromosome-level (complete) genome was also sequenced in this study. Furthermore, the two dominant Endozoicomonas species had different potentials to scavenge reactive oxygen species, suggesting potential differences in responding to the environment. Differential capabilities of dominant members of the microbiome to respond to environmental change can (i) provide distinct advantages or disadvantages to coral hosts when subjected to changing environmental conditions and (ii) have positive or negative implications for future reefs. IMPORTANCE The coral microbiome has been known to play a crucial role in host health. In recent years, we have known that the coral microbiome changes in response to external stressors and that coral hosts structure their microbiome in a host-specific manner. However, an important internal factor, the ability of microbiome members to respond to change, has been often neglected. In this study, we combine metabarcoding, culturing, and genomics to delineate the differential ability of two dominant Endozoicomonas species, including a novel “Ca. Endozoicomonas penghunesis” 4G, to respond to change in the environment following a reciprocal transplant experiment.
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spelling pubmed-94265842022-08-31 Microbiome Restructuring: Dominant Coral Bacterium Endozoicomonas Species Respond Differentially to Environmental Changes Tandon, Kshitij Chiou, Yu-Jing Yu, Sheng-Ping Hsieh, Hernyi Justin Lu, Chih-Ying Hsu, Ming-Tsung Chiang, Pei-Wen Chen, Hsing-Ju Wada, Naohisa Tang, Sen-Lin mSystems Research Article Bacteria in the coral microbiome play a crucial role in determining coral health and fitness, and the coral host often restructures its microbiome composition in response to external factors. An important but often neglected factor determining this microbiome restructuring is the ability of microbiome members to respond to changes in the environment. To address this issue, we examined how the microbiome structure of Acropora muricata corals changed over 9 months following a reciprocal transplant experiment. Using a combination of metabarcoding, genomics, and comparative genomics approaches, we found that coral colonies separated by a small distance harbored different dominant Endozoicomonas-related phylotypes belonging to two different species, including a novel species, “Candidatus Endozoicomonas penghunesis” 4G, whose chromosome-level (complete) genome was also sequenced in this study. Furthermore, the two dominant Endozoicomonas species had different potentials to scavenge reactive oxygen species, suggesting potential differences in responding to the environment. Differential capabilities of dominant members of the microbiome to respond to environmental change can (i) provide distinct advantages or disadvantages to coral hosts when subjected to changing environmental conditions and (ii) have positive or negative implications for future reefs. IMPORTANCE The coral microbiome has been known to play a crucial role in host health. In recent years, we have known that the coral microbiome changes in response to external stressors and that coral hosts structure their microbiome in a host-specific manner. However, an important internal factor, the ability of microbiome members to respond to change, has been often neglected. In this study, we combine metabarcoding, culturing, and genomics to delineate the differential ability of two dominant Endozoicomonas species, including a novel “Ca. Endozoicomonas penghunesis” 4G, to respond to change in the environment following a reciprocal transplant experiment. American Society for Microbiology 2022-06-15 /pmc/articles/PMC9426584/ /pubmed/35703535 http://dx.doi.org/10.1128/msystems.00359-22 Text en Copyright © 2022 Tandon et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Tandon, Kshitij
Chiou, Yu-Jing
Yu, Sheng-Ping
Hsieh, Hernyi Justin
Lu, Chih-Ying
Hsu, Ming-Tsung
Chiang, Pei-Wen
Chen, Hsing-Ju
Wada, Naohisa
Tang, Sen-Lin
Microbiome Restructuring: Dominant Coral Bacterium Endozoicomonas Species Respond Differentially to Environmental Changes
title Microbiome Restructuring: Dominant Coral Bacterium Endozoicomonas Species Respond Differentially to Environmental Changes
title_full Microbiome Restructuring: Dominant Coral Bacterium Endozoicomonas Species Respond Differentially to Environmental Changes
title_fullStr Microbiome Restructuring: Dominant Coral Bacterium Endozoicomonas Species Respond Differentially to Environmental Changes
title_full_unstemmed Microbiome Restructuring: Dominant Coral Bacterium Endozoicomonas Species Respond Differentially to Environmental Changes
title_short Microbiome Restructuring: Dominant Coral Bacterium Endozoicomonas Species Respond Differentially to Environmental Changes
title_sort microbiome restructuring: dominant coral bacterium endozoicomonas species respond differentially to environmental changes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9426584/
https://www.ncbi.nlm.nih.gov/pubmed/35703535
http://dx.doi.org/10.1128/msystems.00359-22
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