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Potential application of TurboID-based proximity labeling in studying the protein interaction network in plant response to abiotic stress

Abiotic stresses are major environmental conditions that reduce plant growth, productivity and quality. Protein-protein interaction (PPI) approaches can be used to screen stress-responsive proteins and reveal the mechanisms of protein response to various abiotic stresses. Biotin-based proximity labe...

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Autores principales: Zhang, Kaixin, Li, Yinyin, Huang, Tengbo, Li, Ziwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9426856/
https://www.ncbi.nlm.nih.gov/pubmed/36051300
http://dx.doi.org/10.3389/fpls.2022.974598
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author Zhang, Kaixin
Li, Yinyin
Huang, Tengbo
Li, Ziwei
author_facet Zhang, Kaixin
Li, Yinyin
Huang, Tengbo
Li, Ziwei
author_sort Zhang, Kaixin
collection PubMed
description Abiotic stresses are major environmental conditions that reduce plant growth, productivity and quality. Protein-protein interaction (PPI) approaches can be used to screen stress-responsive proteins and reveal the mechanisms of protein response to various abiotic stresses. Biotin-based proximity labeling (PL) is a recently developed technique to label proximal proteins of a target protein. TurboID, a biotin ligase produced by directed evolution, has the advantages of non-toxicity, time-saving and high catalytic efficiency compared to other classic protein-labeling enzymes. TurboID-based PL has been successfully applied in animal, microorganism and plant systems, particularly to screen transient or weak protein interactions, and detect spatially or temporally restricted local proteomes in living cells. This review concludes classic PPI approaches in plant response to abiotic stresses and their limitations for identifying complex network of regulatory proteins of plant abiotic stresses, and introduces the working mechanism of TurboID-based PL, as well as its feasibility and advantages in plant abiotic stress research. We hope the information summarized in this article can serve as technical references for further understanding the regulation of plant adaptation to abiotic stress at the protein level.
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spelling pubmed-94268562022-08-31 Potential application of TurboID-based proximity labeling in studying the protein interaction network in plant response to abiotic stress Zhang, Kaixin Li, Yinyin Huang, Tengbo Li, Ziwei Front Plant Sci Plant Science Abiotic stresses are major environmental conditions that reduce plant growth, productivity and quality. Protein-protein interaction (PPI) approaches can be used to screen stress-responsive proteins and reveal the mechanisms of protein response to various abiotic stresses. Biotin-based proximity labeling (PL) is a recently developed technique to label proximal proteins of a target protein. TurboID, a biotin ligase produced by directed evolution, has the advantages of non-toxicity, time-saving and high catalytic efficiency compared to other classic protein-labeling enzymes. TurboID-based PL has been successfully applied in animal, microorganism and plant systems, particularly to screen transient or weak protein interactions, and detect spatially or temporally restricted local proteomes in living cells. This review concludes classic PPI approaches in plant response to abiotic stresses and their limitations for identifying complex network of regulatory proteins of plant abiotic stresses, and introduces the working mechanism of TurboID-based PL, as well as its feasibility and advantages in plant abiotic stress research. We hope the information summarized in this article can serve as technical references for further understanding the regulation of plant adaptation to abiotic stress at the protein level. Frontiers Media S.A. 2022-08-16 /pmc/articles/PMC9426856/ /pubmed/36051300 http://dx.doi.org/10.3389/fpls.2022.974598 Text en Copyright © 2022 Zhang, Li, Huang and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zhang, Kaixin
Li, Yinyin
Huang, Tengbo
Li, Ziwei
Potential application of TurboID-based proximity labeling in studying the protein interaction network in plant response to abiotic stress
title Potential application of TurboID-based proximity labeling in studying the protein interaction network in plant response to abiotic stress
title_full Potential application of TurboID-based proximity labeling in studying the protein interaction network in plant response to abiotic stress
title_fullStr Potential application of TurboID-based proximity labeling in studying the protein interaction network in plant response to abiotic stress
title_full_unstemmed Potential application of TurboID-based proximity labeling in studying the protein interaction network in plant response to abiotic stress
title_short Potential application of TurboID-based proximity labeling in studying the protein interaction network in plant response to abiotic stress
title_sort potential application of turboid-based proximity labeling in studying the protein interaction network in plant response to abiotic stress
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9426856/
https://www.ncbi.nlm.nih.gov/pubmed/36051300
http://dx.doi.org/10.3389/fpls.2022.974598
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