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Human neural stem cells drug product: Microsatellite instability analysis
INTRODUCTION: In central nervous system neurodegenerative disorders, stem cell-based therapies should be considered as a promising therapeutic approach. The safe use of human Neural Stem Cells (hNSCs) for the treatment of several neurological diseases is currently under evaluation of phase I/II clin...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9426914/ https://www.ncbi.nlm.nih.gov/pubmed/36040977 http://dx.doi.org/10.1371/journal.pone.0273679 |
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author | Grespi, Valentina Caprera, Cecilia Ricciolini, Claudia Bicchi, Ilaria Muzi, Gianmarco Corsi, Matteo Ascani, Stefano Vescovi, Angelo Luigi Gelati, Maurizio |
author_facet | Grespi, Valentina Caprera, Cecilia Ricciolini, Claudia Bicchi, Ilaria Muzi, Gianmarco Corsi, Matteo Ascani, Stefano Vescovi, Angelo Luigi Gelati, Maurizio |
author_sort | Grespi, Valentina |
collection | PubMed |
description | INTRODUCTION: In central nervous system neurodegenerative disorders, stem cell-based therapies should be considered as a promising therapeutic approach. The safe use of human Neural Stem Cells (hNSCs) for the treatment of several neurological diseases is currently under evaluation of phase I/II clinical trials. Clinical application of hNSCs require the development of GMP standardized protocols capable of generating high quantities of reproducible and well characterized stem cells bearing stable functional and genetic properties. AIM: The aim of this study was to evaluate possible instabilities or modifications of the microsatellite loci in different culture passages because high culture passages represent an in vitro replicative stress leading to senescence. Experimental method: The hNSCs were characterized at different culture time points, from passage 2 to passage 25, by genetic typing at ten microsatellite loci. CONCLUSION: We showed that genetic stability at microsatellite loci is maintained by the cells even at high passages adding a further demonstration of the safety of our hNSCs GMP culture method. |
format | Online Article Text |
id | pubmed-9426914 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-94269142022-08-31 Human neural stem cells drug product: Microsatellite instability analysis Grespi, Valentina Caprera, Cecilia Ricciolini, Claudia Bicchi, Ilaria Muzi, Gianmarco Corsi, Matteo Ascani, Stefano Vescovi, Angelo Luigi Gelati, Maurizio PLoS One Research Article INTRODUCTION: In central nervous system neurodegenerative disorders, stem cell-based therapies should be considered as a promising therapeutic approach. The safe use of human Neural Stem Cells (hNSCs) for the treatment of several neurological diseases is currently under evaluation of phase I/II clinical trials. Clinical application of hNSCs require the development of GMP standardized protocols capable of generating high quantities of reproducible and well characterized stem cells bearing stable functional and genetic properties. AIM: The aim of this study was to evaluate possible instabilities or modifications of the microsatellite loci in different culture passages because high culture passages represent an in vitro replicative stress leading to senescence. Experimental method: The hNSCs were characterized at different culture time points, from passage 2 to passage 25, by genetic typing at ten microsatellite loci. CONCLUSION: We showed that genetic stability at microsatellite loci is maintained by the cells even at high passages adding a further demonstration of the safety of our hNSCs GMP culture method. Public Library of Science 2022-08-30 /pmc/articles/PMC9426914/ /pubmed/36040977 http://dx.doi.org/10.1371/journal.pone.0273679 Text en © 2022 Grespi et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Grespi, Valentina Caprera, Cecilia Ricciolini, Claudia Bicchi, Ilaria Muzi, Gianmarco Corsi, Matteo Ascani, Stefano Vescovi, Angelo Luigi Gelati, Maurizio Human neural stem cells drug product: Microsatellite instability analysis |
title | Human neural stem cells drug product: Microsatellite instability analysis |
title_full | Human neural stem cells drug product: Microsatellite instability analysis |
title_fullStr | Human neural stem cells drug product: Microsatellite instability analysis |
title_full_unstemmed | Human neural stem cells drug product: Microsatellite instability analysis |
title_short | Human neural stem cells drug product: Microsatellite instability analysis |
title_sort | human neural stem cells drug product: microsatellite instability analysis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9426914/ https://www.ncbi.nlm.nih.gov/pubmed/36040977 http://dx.doi.org/10.1371/journal.pone.0273679 |
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