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Clerodendrum wallichii Merr Methanol Extract Protected Alcohol-Induced Liver Injury in Sprague-Dawley Rats by Modulating Antioxidant Enzymes
MATERIALS AND METHODS: An oral acute toxicity study was carried out following OECD guidelines. Hepatotoxicity was induced by the administration of ethanol for 4 weeks. Hepatic enzymes and oxidative stress biomarkers were determined using commercial diagnostic kits. RESULTS: Treatment of rats with ME...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9427254/ https://www.ncbi.nlm.nih.gov/pubmed/36051496 http://dx.doi.org/10.1155/2022/5635048 |
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author | Tian, Yujian Liang, Ning Jing, Tao Yuan, Fang Sarker, Md. Moklesur Rahman Alam Maruf, Mohammad Rifat Chen, Shuai |
author_facet | Tian, Yujian Liang, Ning Jing, Tao Yuan, Fang Sarker, Md. Moklesur Rahman Alam Maruf, Mohammad Rifat Chen, Shuai |
author_sort | Tian, Yujian |
collection | PubMed |
description | MATERIALS AND METHODS: An oral acute toxicity study was carried out following OECD guidelines. Hepatotoxicity was induced by the administration of ethanol for 4 weeks. Hepatic enzymes and oxidative stress biomarkers were determined using commercial diagnostic kits. RESULTS: Treatment of rats with MECW (800 mg/kg) showed the highest reduction of body weight (4.76 ± 0.372 vs. 16.92 ± 0.846) and liver weight (3.06 ± 0.128 vs 5.55 ± 0.311). Treatment of rats with MECW at 200, 400, 600, 800, and 1000 mg/kg significantly ((∗∗)p < 0.01) reduced SGPT. Similarly, serum SGOT and ALP were significantly decreased by MECW (200, 400, 600, 800, and 1000 mg/kg). All used doses of MECW significantly increased antioxidant enzymes GSH and SOD. MECW (600 and 800 mg/kg) significantly promoted CAT levels in liver tissues; whereas, it significantly diminished oxidative biomarker, MDA. Histopathological observations of the liver showed improvement in the architecture of hepatic cells having signs of protection with a reduced number of inflammatory cells, vascular degeneration and congestion, cellular degeneration, necrosis, and significant reduction of fatty cells accumulation. Acute toxicity study resulted in the well-tolerability and safety of used doses of MECW (200–1000 mg/kg) in rats. CONCLUSION: Our study clearly demonstrated the hepatoprotective effect of Clerodendrum wallichii extract against ethanol-induced liver injury in the laboratory rats model. |
format | Online Article Text |
id | pubmed-9427254 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-94272542022-08-31 Clerodendrum wallichii Merr Methanol Extract Protected Alcohol-Induced Liver Injury in Sprague-Dawley Rats by Modulating Antioxidant Enzymes Tian, Yujian Liang, Ning Jing, Tao Yuan, Fang Sarker, Md. Moklesur Rahman Alam Maruf, Mohammad Rifat Chen, Shuai Evid Based Complement Alternat Med Research Article MATERIALS AND METHODS: An oral acute toxicity study was carried out following OECD guidelines. Hepatotoxicity was induced by the administration of ethanol for 4 weeks. Hepatic enzymes and oxidative stress biomarkers were determined using commercial diagnostic kits. RESULTS: Treatment of rats with MECW (800 mg/kg) showed the highest reduction of body weight (4.76 ± 0.372 vs. 16.92 ± 0.846) and liver weight (3.06 ± 0.128 vs 5.55 ± 0.311). Treatment of rats with MECW at 200, 400, 600, 800, and 1000 mg/kg significantly ((∗∗)p < 0.01) reduced SGPT. Similarly, serum SGOT and ALP were significantly decreased by MECW (200, 400, 600, 800, and 1000 mg/kg). All used doses of MECW significantly increased antioxidant enzymes GSH and SOD. MECW (600 and 800 mg/kg) significantly promoted CAT levels in liver tissues; whereas, it significantly diminished oxidative biomarker, MDA. Histopathological observations of the liver showed improvement in the architecture of hepatic cells having signs of protection with a reduced number of inflammatory cells, vascular degeneration and congestion, cellular degeneration, necrosis, and significant reduction of fatty cells accumulation. Acute toxicity study resulted in the well-tolerability and safety of used doses of MECW (200–1000 mg/kg) in rats. CONCLUSION: Our study clearly demonstrated the hepatoprotective effect of Clerodendrum wallichii extract against ethanol-induced liver injury in the laboratory rats model. Hindawi 2022-08-23 /pmc/articles/PMC9427254/ /pubmed/36051496 http://dx.doi.org/10.1155/2022/5635048 Text en Copyright © 2022 Yujian Tian et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Tian, Yujian Liang, Ning Jing, Tao Yuan, Fang Sarker, Md. Moklesur Rahman Alam Maruf, Mohammad Rifat Chen, Shuai Clerodendrum wallichii Merr Methanol Extract Protected Alcohol-Induced Liver Injury in Sprague-Dawley Rats by Modulating Antioxidant Enzymes |
title |
Clerodendrum wallichii Merr Methanol Extract Protected Alcohol-Induced Liver Injury in Sprague-Dawley Rats by Modulating Antioxidant Enzymes |
title_full |
Clerodendrum wallichii Merr Methanol Extract Protected Alcohol-Induced Liver Injury in Sprague-Dawley Rats by Modulating Antioxidant Enzymes |
title_fullStr |
Clerodendrum wallichii Merr Methanol Extract Protected Alcohol-Induced Liver Injury in Sprague-Dawley Rats by Modulating Antioxidant Enzymes |
title_full_unstemmed |
Clerodendrum wallichii Merr Methanol Extract Protected Alcohol-Induced Liver Injury in Sprague-Dawley Rats by Modulating Antioxidant Enzymes |
title_short |
Clerodendrum wallichii Merr Methanol Extract Protected Alcohol-Induced Liver Injury in Sprague-Dawley Rats by Modulating Antioxidant Enzymes |
title_sort | clerodendrum wallichii merr methanol extract protected alcohol-induced liver injury in sprague-dawley rats by modulating antioxidant enzymes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9427254/ https://www.ncbi.nlm.nih.gov/pubmed/36051496 http://dx.doi.org/10.1155/2022/5635048 |
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