Cargando…

Cardioprotective Activity of Cassia fistula L. Bark Extract in Isoproterenol-Induced Myocardial Infarction Rat Model

Cassia fistula Linn, generally recognized as Indian laburnum, is one of the ancient trees in the Indian subcontinent used for its ornamental and diverse medicinal properties. It is known for its ethnic medicinal uses in inflammatory and infectious pathologies such as antihelmintic, purgative, carmin...

Descripción completa

Detalles Bibliográficos
Autores principales: Kushwah, Ajay Singh, Mittal, Roopal, Kumar, Manish, Kaur, Gurpreet, Goel, Prerna, Sharma, Rahul Kumar, Kabra, Atul, Nainwal, Lalit Mohan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9427257/
https://www.ncbi.nlm.nih.gov/pubmed/36051494
http://dx.doi.org/10.1155/2022/6874281
_version_ 1784778857865281536
author Kushwah, Ajay Singh
Mittal, Roopal
Kumar, Manish
Kaur, Gurpreet
Goel, Prerna
Sharma, Rahul Kumar
Kabra, Atul
Nainwal, Lalit Mohan
author_facet Kushwah, Ajay Singh
Mittal, Roopal
Kumar, Manish
Kaur, Gurpreet
Goel, Prerna
Sharma, Rahul Kumar
Kabra, Atul
Nainwal, Lalit Mohan
author_sort Kushwah, Ajay Singh
collection PubMed
description Cassia fistula Linn, generally recognized as Indian laburnum, is one of the ancient trees in the Indian subcontinent used for its ornamental and diverse medicinal properties. It is known for its ethnic medicinal uses in inflammatory and infectious pathologies such as antihelmintic, purgative, carminative, antipyretic, expectorant, analgesic, laxative, antiseptic, and antidote against snake poison. The Cassia bark is rich in anthraquinones, flavanols glycosides, and sitosterols, which renders it cardioprotective properties. The existing experiments were designed to assess the potential of Cassia fistula bark against isoproterenol (ISP)-induced cardiotoxicity in rats, which has not been validated yet. The bark was successively extracted with five different solvents, and each extract was subjected to in vitro antioxidant studies. Further acute oral toxicity assays were carried out preceding in vivo myocardial studies. Cardiotoxicity-inducing agent, ISP, was administrated to the rats for two consecutive days (8th and 9th). Based on in vitro studies, the Cassia fistula methanolic extract (CFME) was administered in two doses: CFME-LD (lower dose 250 mg/kg) and CFME-HD (high dose 500 mg/kg) separately. It was found that CFME produced a substantial decrease in lipid peroxidation and an increase in antioxidants in myocardial tissues. CFME abrogated the levels of triglyceride and total cholesterol with a decrease in alanine transaminase (ALT) and aspartate transaminase (AST) activity in serum at both doses. 2,3,5-Triphenyltetrazolium chloride (TTC) staining and histopathology also revealed the protective effects of CFME against ISP-induced myocardial infarction. The study showed the significant role of the CFME as a strong antioxidant and cardioprotective action in ISP-induced toxicity.
format Online
Article
Text
id pubmed-9427257
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-94272572022-08-31 Cardioprotective Activity of Cassia fistula L. Bark Extract in Isoproterenol-Induced Myocardial Infarction Rat Model Kushwah, Ajay Singh Mittal, Roopal Kumar, Manish Kaur, Gurpreet Goel, Prerna Sharma, Rahul Kumar Kabra, Atul Nainwal, Lalit Mohan Evid Based Complement Alternat Med Research Article Cassia fistula Linn, generally recognized as Indian laburnum, is one of the ancient trees in the Indian subcontinent used for its ornamental and diverse medicinal properties. It is known for its ethnic medicinal uses in inflammatory and infectious pathologies such as antihelmintic, purgative, carminative, antipyretic, expectorant, analgesic, laxative, antiseptic, and antidote against snake poison. The Cassia bark is rich in anthraquinones, flavanols glycosides, and sitosterols, which renders it cardioprotective properties. The existing experiments were designed to assess the potential of Cassia fistula bark against isoproterenol (ISP)-induced cardiotoxicity in rats, which has not been validated yet. The bark was successively extracted with five different solvents, and each extract was subjected to in vitro antioxidant studies. Further acute oral toxicity assays were carried out preceding in vivo myocardial studies. Cardiotoxicity-inducing agent, ISP, was administrated to the rats for two consecutive days (8th and 9th). Based on in vitro studies, the Cassia fistula methanolic extract (CFME) was administered in two doses: CFME-LD (lower dose 250 mg/kg) and CFME-HD (high dose 500 mg/kg) separately. It was found that CFME produced a substantial decrease in lipid peroxidation and an increase in antioxidants in myocardial tissues. CFME abrogated the levels of triglyceride and total cholesterol with a decrease in alanine transaminase (ALT) and aspartate transaminase (AST) activity in serum at both doses. 2,3,5-Triphenyltetrazolium chloride (TTC) staining and histopathology also revealed the protective effects of CFME against ISP-induced myocardial infarction. The study showed the significant role of the CFME as a strong antioxidant and cardioprotective action in ISP-induced toxicity. Hindawi 2022-08-23 /pmc/articles/PMC9427257/ /pubmed/36051494 http://dx.doi.org/10.1155/2022/6874281 Text en Copyright © 2022 Ajay Singh Kushwah et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kushwah, Ajay Singh
Mittal, Roopal
Kumar, Manish
Kaur, Gurpreet
Goel, Prerna
Sharma, Rahul Kumar
Kabra, Atul
Nainwal, Lalit Mohan
Cardioprotective Activity of Cassia fistula L. Bark Extract in Isoproterenol-Induced Myocardial Infarction Rat Model
title Cardioprotective Activity of Cassia fistula L. Bark Extract in Isoproterenol-Induced Myocardial Infarction Rat Model
title_full Cardioprotective Activity of Cassia fistula L. Bark Extract in Isoproterenol-Induced Myocardial Infarction Rat Model
title_fullStr Cardioprotective Activity of Cassia fistula L. Bark Extract in Isoproterenol-Induced Myocardial Infarction Rat Model
title_full_unstemmed Cardioprotective Activity of Cassia fistula L. Bark Extract in Isoproterenol-Induced Myocardial Infarction Rat Model
title_short Cardioprotective Activity of Cassia fistula L. Bark Extract in Isoproterenol-Induced Myocardial Infarction Rat Model
title_sort cardioprotective activity of cassia fistula l. bark extract in isoproterenol-induced myocardial infarction rat model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9427257/
https://www.ncbi.nlm.nih.gov/pubmed/36051494
http://dx.doi.org/10.1155/2022/6874281
work_keys_str_mv AT kushwahajaysingh cardioprotectiveactivityofcassiafistulalbarkextractinisoproterenolinducedmyocardialinfarctionratmodel
AT mittalroopal cardioprotectiveactivityofcassiafistulalbarkextractinisoproterenolinducedmyocardialinfarctionratmodel
AT kumarmanish cardioprotectiveactivityofcassiafistulalbarkextractinisoproterenolinducedmyocardialinfarctionratmodel
AT kaurgurpreet cardioprotectiveactivityofcassiafistulalbarkextractinisoproterenolinducedmyocardialinfarctionratmodel
AT goelprerna cardioprotectiveactivityofcassiafistulalbarkextractinisoproterenolinducedmyocardialinfarctionratmodel
AT sharmarahulkumar cardioprotectiveactivityofcassiafistulalbarkextractinisoproterenolinducedmyocardialinfarctionratmodel
AT kabraatul cardioprotectiveactivityofcassiafistulalbarkextractinisoproterenolinducedmyocardialinfarctionratmodel
AT nainwallalitmohan cardioprotectiveactivityofcassiafistulalbarkextractinisoproterenolinducedmyocardialinfarctionratmodel