Cargando…

Zoonotic potential of multidrug-resistant extraintestinal pathogenic Escherichia coli obtained from healthy poultry carcasses in Salvador, Brazil

The zoonotic potential to cause human and/or animal infections among multidrug-resistant extraintestinal pathogenic Escherichia coli from avian origin was investigated. Twenty-seven extraintestinal pathogenic E. coli isolates containing the increased survival gene (iss) were obtained from the livers...

Descripción completa

Detalles Bibliográficos
Autores principales: Lima-Filho, José Vitor, Martins, Liliane Vilela, Nascimento, Danielle Cristina de Oliveira, Ventura, Roberta Ferreira, Batista, Jacqueline Ellen Camelo, Silva, Ayrles Fernanda Brandão, Ralph, Maria Taciana, Vaz, Renata Valença, Rabello, Carlos Boa-Viagem, Silva, Isabella de Matos Mendes da, Evêncio-Neto, Joaquim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9427334/
https://www.ncbi.nlm.nih.gov/pubmed/23290470
http://dx.doi.org/10.1016/j.bjid.2012.09.004
_version_ 1784778876922101760
author Lima-Filho, José Vitor
Martins, Liliane Vilela
Nascimento, Danielle Cristina de Oliveira
Ventura, Roberta Ferreira
Batista, Jacqueline Ellen Camelo
Silva, Ayrles Fernanda Brandão
Ralph, Maria Taciana
Vaz, Renata Valença
Rabello, Carlos Boa-Viagem
Silva, Isabella de Matos Mendes da
Evêncio-Neto, Joaquim
author_facet Lima-Filho, José Vitor
Martins, Liliane Vilela
Nascimento, Danielle Cristina de Oliveira
Ventura, Roberta Ferreira
Batista, Jacqueline Ellen Camelo
Silva, Ayrles Fernanda Brandão
Ralph, Maria Taciana
Vaz, Renata Valença
Rabello, Carlos Boa-Viagem
Silva, Isabella de Matos Mendes da
Evêncio-Neto, Joaquim
author_sort Lima-Filho, José Vitor
collection PubMed
description The zoonotic potential to cause human and/or animal infections among multidrug-resistant extraintestinal pathogenic Escherichia coli from avian origin was investigated. Twenty-seven extraintestinal pathogenic E. coli isolates containing the increased survival gene (iss) were obtained from the livers of healthy and diseased poultry carcasses at two slaughterhouses in Salvador, northeastern Brazil. The antimicrobial resistance-susceptibility profiles were conducted with antibiotics of avian and/or human use by the standardized disc-diffusion method. Antimicrobial resistance was higher for levofloxacin (51.8%), amoxicillin/clavulanic acid (70.4%), ampicillin (81.5%), cefalotin (88.8%), tetracycline (100%) and streptomycin (100%). The minimum inhibitory concentrations above the resistance breakpoints of doxycycline, neomycin, oxytetracycline and enrofloxacin reached, respectively, 88.0%, 100%, 75% and 91.7% of the isolates. Strains with high and low antimicrobial resistance were i.p. administered to Swiss mice, and histopathological examination was carried out seven days after infection. Resistance to goat and human serum complement was also evaluated. The results show that Swiss mice challenged with strain 2B (resistant to 11 antimicrobials) provoked a severe degeneration of hepatocytes besides lymphocytic infiltration in the liver, whereas the spleen showed areas of degeneration of the white and red pulp. Conversely, the spleen and liver of mice challenged with strain 4A (resistant to two antimicrobials) were morphologically preserved. In addition, complement resistance to goat and human serum was high for strain 2B and low for strain 4A. Our data show that multidrug resistance and pathogenesis can be correlated in extraintestinal pathogenic E. coli strains obtained from apparently healthy poultry carcasses, increasing the risk for human public healthy.
format Online
Article
Text
id pubmed-9427334
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-94273342022-09-01 Zoonotic potential of multidrug-resistant extraintestinal pathogenic Escherichia coli obtained from healthy poultry carcasses in Salvador, Brazil Lima-Filho, José Vitor Martins, Liliane Vilela Nascimento, Danielle Cristina de Oliveira Ventura, Roberta Ferreira Batista, Jacqueline Ellen Camelo Silva, Ayrles Fernanda Brandão Ralph, Maria Taciana Vaz, Renata Valença Rabello, Carlos Boa-Viagem Silva, Isabella de Matos Mendes da Evêncio-Neto, Joaquim Braz J Infect Dis Original Article The zoonotic potential to cause human and/or animal infections among multidrug-resistant extraintestinal pathogenic Escherichia coli from avian origin was investigated. Twenty-seven extraintestinal pathogenic E. coli isolates containing the increased survival gene (iss) were obtained from the livers of healthy and diseased poultry carcasses at two slaughterhouses in Salvador, northeastern Brazil. The antimicrobial resistance-susceptibility profiles were conducted with antibiotics of avian and/or human use by the standardized disc-diffusion method. Antimicrobial resistance was higher for levofloxacin (51.8%), amoxicillin/clavulanic acid (70.4%), ampicillin (81.5%), cefalotin (88.8%), tetracycline (100%) and streptomycin (100%). The minimum inhibitory concentrations above the resistance breakpoints of doxycycline, neomycin, oxytetracycline and enrofloxacin reached, respectively, 88.0%, 100%, 75% and 91.7% of the isolates. Strains with high and low antimicrobial resistance were i.p. administered to Swiss mice, and histopathological examination was carried out seven days after infection. Resistance to goat and human serum complement was also evaluated. The results show that Swiss mice challenged with strain 2B (resistant to 11 antimicrobials) provoked a severe degeneration of hepatocytes besides lymphocytic infiltration in the liver, whereas the spleen showed areas of degeneration of the white and red pulp. Conversely, the spleen and liver of mice challenged with strain 4A (resistant to two antimicrobials) were morphologically preserved. In addition, complement resistance to goat and human serum was high for strain 2B and low for strain 4A. Our data show that multidrug resistance and pathogenesis can be correlated in extraintestinal pathogenic E. coli strains obtained from apparently healthy poultry carcasses, increasing the risk for human public healthy. Elsevier 2013-01-03 /pmc/articles/PMC9427334/ /pubmed/23290470 http://dx.doi.org/10.1016/j.bjid.2012.09.004 Text en © 2013 Elsevier Editora Ltda. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Lima-Filho, José Vitor
Martins, Liliane Vilela
Nascimento, Danielle Cristina de Oliveira
Ventura, Roberta Ferreira
Batista, Jacqueline Ellen Camelo
Silva, Ayrles Fernanda Brandão
Ralph, Maria Taciana
Vaz, Renata Valença
Rabello, Carlos Boa-Viagem
Silva, Isabella de Matos Mendes da
Evêncio-Neto, Joaquim
Zoonotic potential of multidrug-resistant extraintestinal pathogenic Escherichia coli obtained from healthy poultry carcasses in Salvador, Brazil
title Zoonotic potential of multidrug-resistant extraintestinal pathogenic Escherichia coli obtained from healthy poultry carcasses in Salvador, Brazil
title_full Zoonotic potential of multidrug-resistant extraintestinal pathogenic Escherichia coli obtained from healthy poultry carcasses in Salvador, Brazil
title_fullStr Zoonotic potential of multidrug-resistant extraintestinal pathogenic Escherichia coli obtained from healthy poultry carcasses in Salvador, Brazil
title_full_unstemmed Zoonotic potential of multidrug-resistant extraintestinal pathogenic Escherichia coli obtained from healthy poultry carcasses in Salvador, Brazil
title_short Zoonotic potential of multidrug-resistant extraintestinal pathogenic Escherichia coli obtained from healthy poultry carcasses in Salvador, Brazil
title_sort zoonotic potential of multidrug-resistant extraintestinal pathogenic escherichia coli obtained from healthy poultry carcasses in salvador, brazil
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9427334/
https://www.ncbi.nlm.nih.gov/pubmed/23290470
http://dx.doi.org/10.1016/j.bjid.2012.09.004
work_keys_str_mv AT limafilhojosevitor zoonoticpotentialofmultidrugresistantextraintestinalpathogenicescherichiacoliobtainedfromhealthypoultrycarcassesinsalvadorbrazil
AT martinslilianevilela zoonoticpotentialofmultidrugresistantextraintestinalpathogenicescherichiacoliobtainedfromhealthypoultrycarcassesinsalvadorbrazil
AT nascimentodaniellecristinadeoliveira zoonoticpotentialofmultidrugresistantextraintestinalpathogenicescherichiacoliobtainedfromhealthypoultrycarcassesinsalvadorbrazil
AT venturarobertaferreira zoonoticpotentialofmultidrugresistantextraintestinalpathogenicescherichiacoliobtainedfromhealthypoultrycarcassesinsalvadorbrazil
AT batistajacquelineellencamelo zoonoticpotentialofmultidrugresistantextraintestinalpathogenicescherichiacoliobtainedfromhealthypoultrycarcassesinsalvadorbrazil
AT silvaayrlesfernandabrandao zoonoticpotentialofmultidrugresistantextraintestinalpathogenicescherichiacoliobtainedfromhealthypoultrycarcassesinsalvadorbrazil
AT ralphmariataciana zoonoticpotentialofmultidrugresistantextraintestinalpathogenicescherichiacoliobtainedfromhealthypoultrycarcassesinsalvadorbrazil
AT vazrenatavalenca zoonoticpotentialofmultidrugresistantextraintestinalpathogenicescherichiacoliobtainedfromhealthypoultrycarcassesinsalvadorbrazil
AT rabellocarlosboaviagem zoonoticpotentialofmultidrugresistantextraintestinalpathogenicescherichiacoliobtainedfromhealthypoultrycarcassesinsalvadorbrazil
AT silvaisabelladematosmendesda zoonoticpotentialofmultidrugresistantextraintestinalpathogenicescherichiacoliobtainedfromhealthypoultrycarcassesinsalvadorbrazil
AT evencionetojoaquim zoonoticpotentialofmultidrugresistantextraintestinalpathogenicescherichiacoliobtainedfromhealthypoultrycarcassesinsalvadorbrazil