Cargando…
A simple, rapid and economic method for detecting multidrug-resistant tuberculosis
OBJECTIVE: To evaluate multiplex allele specific polymerase chain reaction as a rapid molecular tool for detecting multidrug-resistant tuberculosis. METHODS: Based on drug susceptibility testing, 103 isolates were multidrug-resistant tuberculosis and 45 isolates were sensitive to isonicotinylhydrazi...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9427339/ https://www.ncbi.nlm.nih.gov/pubmed/24029439 http://dx.doi.org/10.1016/j.bjid.2013.04.008 |
_version_ | 1784778878164664320 |
---|---|
author | Wang, Xia Jiao, Junhua Xu, Weihua Chai, Xiaoyan Li, Zhenyun Wang, Qingjiang |
author_facet | Wang, Xia Jiao, Junhua Xu, Weihua Chai, Xiaoyan Li, Zhenyun Wang, Qingjiang |
author_sort | Wang, Xia |
collection | PubMed |
description | OBJECTIVE: To evaluate multiplex allele specific polymerase chain reaction as a rapid molecular tool for detecting multidrug-resistant tuberculosis. METHODS: Based on drug susceptibility testing, 103 isolates were multidrug-resistant tuberculosis and 45 isolates were sensitive to isonicotinylhydrazine and rifampin. Primers were designed to target five mutations hotspots that confer resistance to the first-line drugs isoniazid and rifampin, and multiplex allele specific polymerase chain reaction was performed. Whole-genome sequencing confirmed drug resistance mutations identified by multiplex allele specific polymerase chain reaction. RESULTS: DNA sequencing revealed that 68.9% of multidrug-resistant strains have point mutations at codon 315 of the katG gene, 19.8% within the mabA-inhA promoter, and 98.0% at three hotspots within rpoB. Multiplex allele specific polymerase chain reaction detected each of these five mutations, yielding 82.3% sensitivity and 100% specificity for isoniazid resistance, and 97.9% sensitivity and 100% specificity for rifampin resistance as compared to drug susceptibility testing. CONCLUSIONS: The results show that multiplex allele specific polymerase chain reaction is an inexpensive and practical method for rapid detection of multidrug-resistant tuberculosis in developing countries. |
format | Online Article Text |
id | pubmed-9427339 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-94273392022-09-01 A simple, rapid and economic method for detecting multidrug-resistant tuberculosis Wang, Xia Jiao, Junhua Xu, Weihua Chai, Xiaoyan Li, Zhenyun Wang, Qingjiang Braz J Infect Dis Original Article OBJECTIVE: To evaluate multiplex allele specific polymerase chain reaction as a rapid molecular tool for detecting multidrug-resistant tuberculosis. METHODS: Based on drug susceptibility testing, 103 isolates were multidrug-resistant tuberculosis and 45 isolates were sensitive to isonicotinylhydrazine and rifampin. Primers were designed to target five mutations hotspots that confer resistance to the first-line drugs isoniazid and rifampin, and multiplex allele specific polymerase chain reaction was performed. Whole-genome sequencing confirmed drug resistance mutations identified by multiplex allele specific polymerase chain reaction. RESULTS: DNA sequencing revealed that 68.9% of multidrug-resistant strains have point mutations at codon 315 of the katG gene, 19.8% within the mabA-inhA promoter, and 98.0% at three hotspots within rpoB. Multiplex allele specific polymerase chain reaction detected each of these five mutations, yielding 82.3% sensitivity and 100% specificity for isoniazid resistance, and 97.9% sensitivity and 100% specificity for rifampin resistance as compared to drug susceptibility testing. CONCLUSIONS: The results show that multiplex allele specific polymerase chain reaction is an inexpensive and practical method for rapid detection of multidrug-resistant tuberculosis in developing countries. Elsevier 2013-09-09 /pmc/articles/PMC9427339/ /pubmed/24029439 http://dx.doi.org/10.1016/j.bjid.2013.04.008 Text en © 2013 Elsevier Editora Ltda. Este é um artigo Open Access sob a licença de CC BY-NC-ND. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Wang, Xia Jiao, Junhua Xu, Weihua Chai, Xiaoyan Li, Zhenyun Wang, Qingjiang A simple, rapid and economic method for detecting multidrug-resistant tuberculosis |
title | A simple, rapid and economic method for detecting multidrug-resistant tuberculosis |
title_full | A simple, rapid and economic method for detecting multidrug-resistant tuberculosis |
title_fullStr | A simple, rapid and economic method for detecting multidrug-resistant tuberculosis |
title_full_unstemmed | A simple, rapid and economic method for detecting multidrug-resistant tuberculosis |
title_short | A simple, rapid and economic method for detecting multidrug-resistant tuberculosis |
title_sort | simple, rapid and economic method for detecting multidrug-resistant tuberculosis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9427339/ https://www.ncbi.nlm.nih.gov/pubmed/24029439 http://dx.doi.org/10.1016/j.bjid.2013.04.008 |
work_keys_str_mv | AT wangxia asimplerapidandeconomicmethodfordetectingmultidrugresistanttuberculosis AT jiaojunhua asimplerapidandeconomicmethodfordetectingmultidrugresistanttuberculosis AT xuweihua asimplerapidandeconomicmethodfordetectingmultidrugresistanttuberculosis AT chaixiaoyan asimplerapidandeconomicmethodfordetectingmultidrugresistanttuberculosis AT lizhenyun asimplerapidandeconomicmethodfordetectingmultidrugresistanttuberculosis AT wangqingjiang asimplerapidandeconomicmethodfordetectingmultidrugresistanttuberculosis AT wangxia simplerapidandeconomicmethodfordetectingmultidrugresistanttuberculosis AT jiaojunhua simplerapidandeconomicmethodfordetectingmultidrugresistanttuberculosis AT xuweihua simplerapidandeconomicmethodfordetectingmultidrugresistanttuberculosis AT chaixiaoyan simplerapidandeconomicmethodfordetectingmultidrugresistanttuberculosis AT lizhenyun simplerapidandeconomicmethodfordetectingmultidrugresistanttuberculosis AT wangqingjiang simplerapidandeconomicmethodfordetectingmultidrugresistanttuberculosis |