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A simple, rapid and economic method for detecting multidrug-resistant tuberculosis

OBJECTIVE: To evaluate multiplex allele specific polymerase chain reaction as a rapid molecular tool for detecting multidrug-resistant tuberculosis. METHODS: Based on drug susceptibility testing, 103 isolates were multidrug-resistant tuberculosis and 45 isolates were sensitive to isonicotinylhydrazi...

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Autores principales: Wang, Xia, Jiao, Junhua, Xu, Weihua, Chai, Xiaoyan, Li, Zhenyun, Wang, Qingjiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9427339/
https://www.ncbi.nlm.nih.gov/pubmed/24029439
http://dx.doi.org/10.1016/j.bjid.2013.04.008
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author Wang, Xia
Jiao, Junhua
Xu, Weihua
Chai, Xiaoyan
Li, Zhenyun
Wang, Qingjiang
author_facet Wang, Xia
Jiao, Junhua
Xu, Weihua
Chai, Xiaoyan
Li, Zhenyun
Wang, Qingjiang
author_sort Wang, Xia
collection PubMed
description OBJECTIVE: To evaluate multiplex allele specific polymerase chain reaction as a rapid molecular tool for detecting multidrug-resistant tuberculosis. METHODS: Based on drug susceptibility testing, 103 isolates were multidrug-resistant tuberculosis and 45 isolates were sensitive to isonicotinylhydrazine and rifampin. Primers were designed to target five mutations hotspots that confer resistance to the first-line drugs isoniazid and rifampin, and multiplex allele specific polymerase chain reaction was performed. Whole-genome sequencing confirmed drug resistance mutations identified by multiplex allele specific polymerase chain reaction. RESULTS: DNA sequencing revealed that 68.9% of multidrug-resistant strains have point mutations at codon 315 of the katG gene, 19.8% within the mabA-inhA promoter, and 98.0% at three hotspots within rpoB. Multiplex allele specific polymerase chain reaction detected each of these five mutations, yielding 82.3% sensitivity and 100% specificity for isoniazid resistance, and 97.9% sensitivity and 100% specificity for rifampin resistance as compared to drug susceptibility testing. CONCLUSIONS: The results show that multiplex allele specific polymerase chain reaction is an inexpensive and practical method for rapid detection of multidrug-resistant tuberculosis in developing countries.
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spelling pubmed-94273392022-09-01 A simple, rapid and economic method for detecting multidrug-resistant tuberculosis Wang, Xia Jiao, Junhua Xu, Weihua Chai, Xiaoyan Li, Zhenyun Wang, Qingjiang Braz J Infect Dis Original Article OBJECTIVE: To evaluate multiplex allele specific polymerase chain reaction as a rapid molecular tool for detecting multidrug-resistant tuberculosis. METHODS: Based on drug susceptibility testing, 103 isolates were multidrug-resistant tuberculosis and 45 isolates were sensitive to isonicotinylhydrazine and rifampin. Primers were designed to target five mutations hotspots that confer resistance to the first-line drugs isoniazid and rifampin, and multiplex allele specific polymerase chain reaction was performed. Whole-genome sequencing confirmed drug resistance mutations identified by multiplex allele specific polymerase chain reaction. RESULTS: DNA sequencing revealed that 68.9% of multidrug-resistant strains have point mutations at codon 315 of the katG gene, 19.8% within the mabA-inhA promoter, and 98.0% at three hotspots within rpoB. Multiplex allele specific polymerase chain reaction detected each of these five mutations, yielding 82.3% sensitivity and 100% specificity for isoniazid resistance, and 97.9% sensitivity and 100% specificity for rifampin resistance as compared to drug susceptibility testing. CONCLUSIONS: The results show that multiplex allele specific polymerase chain reaction is an inexpensive and practical method for rapid detection of multidrug-resistant tuberculosis in developing countries. Elsevier 2013-09-09 /pmc/articles/PMC9427339/ /pubmed/24029439 http://dx.doi.org/10.1016/j.bjid.2013.04.008 Text en © 2013 Elsevier Editora Ltda. Este é um artigo Open Access sob a licença de CC BY-NC-ND. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Wang, Xia
Jiao, Junhua
Xu, Weihua
Chai, Xiaoyan
Li, Zhenyun
Wang, Qingjiang
A simple, rapid and economic method for detecting multidrug-resistant tuberculosis
title A simple, rapid and economic method for detecting multidrug-resistant tuberculosis
title_full A simple, rapid and economic method for detecting multidrug-resistant tuberculosis
title_fullStr A simple, rapid and economic method for detecting multidrug-resistant tuberculosis
title_full_unstemmed A simple, rapid and economic method for detecting multidrug-resistant tuberculosis
title_short A simple, rapid and economic method for detecting multidrug-resistant tuberculosis
title_sort simple, rapid and economic method for detecting multidrug-resistant tuberculosis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9427339/
https://www.ncbi.nlm.nih.gov/pubmed/24029439
http://dx.doi.org/10.1016/j.bjid.2013.04.008
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