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Dynamic mechanisms of CRISPR interference by Escherichia coli CRISPR-Cas3

Type I CRISPR-Cas3 uses an RNA-guided multi Cas-protein complex, Cascade, which detects and degrades foreign nucleic acids via the helicase-nuclease Cas3 protein. Despite many studies using cryoEM and smFRET, the precise mechanism of Cas3-mediated cleavage and degradation of target DNA remains elusi...

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Detalles Bibliográficos
Autores principales:	Yoshimi, Kazuto, Takeshita, Kohei, Kodera, Noriyuki, Shibumura, Satomi, Yamauchi, Yuko, Omatsu, Mine, Umeda, Kenichi, Kunihiro, Yayoi, Yamamoto, Masaki, Mashimo, Tomoji
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9427990/ https://www.ncbi.nlm.nih.gov/pubmed/36042215 http://dx.doi.org/10.1038/s41467-022-32618-0

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