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Ferroptosis-related gene expression in the pathogenesis of preeclampsia

Background: Preeclampsia (PE) is one of the leading causes of maternal and fetal morbidity and mortality worldwide. Placental oxidative stress has been identified as a major pathway to the development of PE. Ferroptosis is a new form of regulated cell death that is associated with iron metabolism an...

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Autores principales: Ding, Yuzhen, Yang, Xiaofeng, Han, Xiaoxue, Shi, Meiting, Sun, Lu, Liu, Mengyuan, Zhang, Ping, Huang, Zhengrui, Yang, Xiuli, Li, Ruiman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9428486/
https://www.ncbi.nlm.nih.gov/pubmed/36061193
http://dx.doi.org/10.3389/fgene.2022.927869
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author Ding, Yuzhen
Yang, Xiaofeng
Han, Xiaoxue
Shi, Meiting
Sun, Lu
Liu, Mengyuan
Zhang, Ping
Huang, Zhengrui
Yang, Xiuli
Li, Ruiman
author_facet Ding, Yuzhen
Yang, Xiaofeng
Han, Xiaoxue
Shi, Meiting
Sun, Lu
Liu, Mengyuan
Zhang, Ping
Huang, Zhengrui
Yang, Xiuli
Li, Ruiman
author_sort Ding, Yuzhen
collection PubMed
description Background: Preeclampsia (PE) is one of the leading causes of maternal and fetal morbidity and mortality worldwide. Placental oxidative stress has been identified as a major pathway to the development of PE. Ferroptosis is a new form of regulated cell death that is associated with iron metabolism and oxidative stress, and likely mediates PE pathogenesis. The aim of the study was to identify the key molecules involved in ferroptosis to further explore the mechanism of ferroptosis in PE. Methods: Gene expression data and clinical information were downloaded from the GEO database. The limma R package was used to screen differentially expressed genes (DEGs) and intersected with ferroptosis genes. The GO and KEGG pathways were then analyzed. Next, hub genes were identified via weighted gene co-expression network analysis (WGCNA). Receiver operating curves (ROCs) were performed for diagnostic and Pearson’s correlation of hub genes and clinicopathological characteristics. Immunohistochemistry and Western blot analysis were used to verify the expression of hub genes. Results: A total of 3,142 DEGs were identified and 30 ferroptosis-related DEGs were obtained. In addition, ferroptosis-related pathways were enriched by GO and KEGG using DEGs. Two critical modules and six hub genes that were highly related to diagnosis of PE were identified through WGCNA. The analysis of the clinicopathological features showed that NQO1 and SRXN1 were closely correlated with PE characteristics and diagnosis. Finally, Western blot and immunohistochemistry analysis confirmed that the expression of the SRXN1 protein in the placental tissue of patients with PE was significantly elevated, while the expression of NQO1 was significantly decreased. Conclusions: SRXN1 and NQO1 may be key ferroptosis-related proteins in the pathogenesis of PE. The study may provide a theoretical and experimental basis for revealing the pathogenesis of PE and improving the diagnosis of PE.
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spelling pubmed-94284862022-09-01 Ferroptosis-related gene expression in the pathogenesis of preeclampsia Ding, Yuzhen Yang, Xiaofeng Han, Xiaoxue Shi, Meiting Sun, Lu Liu, Mengyuan Zhang, Ping Huang, Zhengrui Yang, Xiuli Li, Ruiman Front Genet Genetics Background: Preeclampsia (PE) is one of the leading causes of maternal and fetal morbidity and mortality worldwide. Placental oxidative stress has been identified as a major pathway to the development of PE. Ferroptosis is a new form of regulated cell death that is associated with iron metabolism and oxidative stress, and likely mediates PE pathogenesis. The aim of the study was to identify the key molecules involved in ferroptosis to further explore the mechanism of ferroptosis in PE. Methods: Gene expression data and clinical information were downloaded from the GEO database. The limma R package was used to screen differentially expressed genes (DEGs) and intersected with ferroptosis genes. The GO and KEGG pathways were then analyzed. Next, hub genes were identified via weighted gene co-expression network analysis (WGCNA). Receiver operating curves (ROCs) were performed for diagnostic and Pearson’s correlation of hub genes and clinicopathological characteristics. Immunohistochemistry and Western blot analysis were used to verify the expression of hub genes. Results: A total of 3,142 DEGs were identified and 30 ferroptosis-related DEGs were obtained. In addition, ferroptosis-related pathways were enriched by GO and KEGG using DEGs. Two critical modules and six hub genes that were highly related to diagnosis of PE were identified through WGCNA. The analysis of the clinicopathological features showed that NQO1 and SRXN1 were closely correlated with PE characteristics and diagnosis. Finally, Western blot and immunohistochemistry analysis confirmed that the expression of the SRXN1 protein in the placental tissue of patients with PE was significantly elevated, while the expression of NQO1 was significantly decreased. Conclusions: SRXN1 and NQO1 may be key ferroptosis-related proteins in the pathogenesis of PE. The study may provide a theoretical and experimental basis for revealing the pathogenesis of PE and improving the diagnosis of PE. Frontiers Media S.A. 2022-08-17 /pmc/articles/PMC9428486/ /pubmed/36061193 http://dx.doi.org/10.3389/fgene.2022.927869 Text en Copyright © 2022 Ding, Yang, Han, Shi, Sun, Liu, Zhang, Huang, Yang and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Ding, Yuzhen
Yang, Xiaofeng
Han, Xiaoxue
Shi, Meiting
Sun, Lu
Liu, Mengyuan
Zhang, Ping
Huang, Zhengrui
Yang, Xiuli
Li, Ruiman
Ferroptosis-related gene expression in the pathogenesis of preeclampsia
title Ferroptosis-related gene expression in the pathogenesis of preeclampsia
title_full Ferroptosis-related gene expression in the pathogenesis of preeclampsia
title_fullStr Ferroptosis-related gene expression in the pathogenesis of preeclampsia
title_full_unstemmed Ferroptosis-related gene expression in the pathogenesis of preeclampsia
title_short Ferroptosis-related gene expression in the pathogenesis of preeclampsia
title_sort ferroptosis-related gene expression in the pathogenesis of preeclampsia
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9428486/
https://www.ncbi.nlm.nih.gov/pubmed/36061193
http://dx.doi.org/10.3389/fgene.2022.927869
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