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Cultivating marine bacteria under laboratory conditions: Overcoming the “unculturable” dogma

Underexplored seawater environments may contain biological resources with potential for new biotechnological applications. Metagenomic techniques revolutionized the study of bacterial communities but culture dependent methods will still be important to help the biodiscovery of new products and enzym...

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Autores principales: Rodrigues, Carlos J. C., de Carvalho, Carla C. C. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9428589/
https://www.ncbi.nlm.nih.gov/pubmed/36061424
http://dx.doi.org/10.3389/fbioe.2022.964589
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author Rodrigues, Carlos J. C.
de Carvalho, Carla C. C. R.
author_facet Rodrigues, Carlos J. C.
de Carvalho, Carla C. C. R.
author_sort Rodrigues, Carlos J. C.
collection PubMed
description Underexplored seawater environments may contain biological resources with potential for new biotechnological applications. Metagenomic techniques revolutionized the study of bacterial communities but culture dependent methods will still be important to help the biodiscovery of new products and enzymes from marine bacteria. In this context, we promoted the growth of bacteria from a marine rock pond by culture dependent techniques and compared the results with culture independent methods. The total number of bacteria and diversity were studied in different agar plate media during 6 weeks. Agar plate counting was of the same order of magnitude of direct microscopy counts. The highest efficiency of cultivation was 45% attained in marine agar medium. Molecular analysis revealed 10 different phyla of which only four were isolated by the culture dependent method. On the other hand, four taxonomic orders were detected by cultivation but not by the molecular technique. These include bacteria from the phyla Bacillota and Actinomycetota. Our study shows that it is possible to grow more than the traditionally considered 1% of bacteria from a seawater sample using standard agar plate techniques and laboratorial conditions. The results also demonstrate the importance of culture methods to grow bacteria not detected by molecular approaches for future biotechnological applications.
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spelling pubmed-94285892022-09-01 Cultivating marine bacteria under laboratory conditions: Overcoming the “unculturable” dogma Rodrigues, Carlos J. C. de Carvalho, Carla C. C. R. Front Bioeng Biotechnol Bioengineering and Biotechnology Underexplored seawater environments may contain biological resources with potential for new biotechnological applications. Metagenomic techniques revolutionized the study of bacterial communities but culture dependent methods will still be important to help the biodiscovery of new products and enzymes from marine bacteria. In this context, we promoted the growth of bacteria from a marine rock pond by culture dependent techniques and compared the results with culture independent methods. The total number of bacteria and diversity were studied in different agar plate media during 6 weeks. Agar plate counting was of the same order of magnitude of direct microscopy counts. The highest efficiency of cultivation was 45% attained in marine agar medium. Molecular analysis revealed 10 different phyla of which only four were isolated by the culture dependent method. On the other hand, four taxonomic orders were detected by cultivation but not by the molecular technique. These include bacteria from the phyla Bacillota and Actinomycetota. Our study shows that it is possible to grow more than the traditionally considered 1% of bacteria from a seawater sample using standard agar plate techniques and laboratorial conditions. The results also demonstrate the importance of culture methods to grow bacteria not detected by molecular approaches for future biotechnological applications. Frontiers Media S.A. 2022-08-17 /pmc/articles/PMC9428589/ /pubmed/36061424 http://dx.doi.org/10.3389/fbioe.2022.964589 Text en Copyright © 2022 Rodrigues and de Carvalho. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Rodrigues, Carlos J. C.
de Carvalho, Carla C. C. R.
Cultivating marine bacteria under laboratory conditions: Overcoming the “unculturable” dogma
title Cultivating marine bacteria under laboratory conditions: Overcoming the “unculturable” dogma
title_full Cultivating marine bacteria under laboratory conditions: Overcoming the “unculturable” dogma
title_fullStr Cultivating marine bacteria under laboratory conditions: Overcoming the “unculturable” dogma
title_full_unstemmed Cultivating marine bacteria under laboratory conditions: Overcoming the “unculturable” dogma
title_short Cultivating marine bacteria under laboratory conditions: Overcoming the “unculturable” dogma
title_sort cultivating marine bacteria under laboratory conditions: overcoming the “unculturable” dogma
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9428589/
https://www.ncbi.nlm.nih.gov/pubmed/36061424
http://dx.doi.org/10.3389/fbioe.2022.964589
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