A collaborative study to establish the national standard for SARS-CoV-2 RNA nucleic acid amplification techniques (NAAT) in Taiwan

The conventional PCR remains a valuable method to detect the newly emergent coronavirus rapidly and accurately. Our investigation aimed to establish the standard materials of SARS-CoV-2 for NAAT detection. We provided formalin-inactivated SARS-CoV-2 and confirmed RNA copy numbers. In addition, the v...

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Detalles Bibliográficos
Autores principales: Lin, Po-Lin, Wu, Ming-Sian, Wu, Po-Chi, Chen, Hsin-Mei, Peng, Yi-Hsuan, Hsu, Jia-Chuan, Wang, Der-Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Alliance for Biological Standardization. Published by Elsevier Ltd. 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9428600/
https://www.ncbi.nlm.nih.gov/pubmed/36085128
http://dx.doi.org/10.1016/j.biologicals.2022.08.002
Descripción
Sumario:The conventional PCR remains a valuable method to detect the newly emergent coronavirus rapidly and accurately. Our investigation aimed to establish the standard materials of SARS-CoV-2 for NAAT detection. We provided formalin-inactivated SARS-CoV-2 and confirmed RNA copy numbers. In addition, the virus genome was confirmed with whole-genome sequencing and identified as Wuhan/WI04/2019. Seven laboratories were invited for this collaborative study, according to the reporting data, we determined the SARS-CoV-2 with the unit of 6.35 Log(10) copies/mL as the national standard. The availability of the national standard (NS) of SARS-CoV-2 will facilitate the standardization and harmonization of SARS-CoV-2 NAAT assays.

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