A collaborative study to establish the national standard for SARS-CoV-2 RNA nucleic acid amplification techniques (NAAT) in Taiwan

The conventional PCR remains a valuable method to detect the newly emergent coronavirus rapidly and accurately. Our investigation aimed to establish the standard materials of SARS-CoV-2 for NAAT detection. We provided formalin-inactivated SARS-CoV-2 and confirmed RNA copy numbers. In addition, the v...

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Autores principales: Lin, Po-Lin, Wu, Ming-Sian, Wu, Po-Chi, Chen, Hsin-Mei, Peng, Yi-Hsuan, Hsu, Jia-Chuan, Wang, Der-Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Alliance for Biological Standardization. Published by Elsevier Ltd. 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9428600/
https://www.ncbi.nlm.nih.gov/pubmed/36085128
http://dx.doi.org/10.1016/j.biologicals.2022.08.002
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author Lin, Po-Lin
Wu, Ming-Sian
Wu, Po-Chi
Chen, Hsin-Mei
Peng, Yi-Hsuan
Hsu, Jia-Chuan
Wang, Der-Yuan
author_facet Lin, Po-Lin
Wu, Ming-Sian
Wu, Po-Chi
Chen, Hsin-Mei
Peng, Yi-Hsuan
Hsu, Jia-Chuan
Wang, Der-Yuan
author_sort Lin, Po-Lin
collection PubMed
description The conventional PCR remains a valuable method to detect the newly emergent coronavirus rapidly and accurately. Our investigation aimed to establish the standard materials of SARS-CoV-2 for NAAT detection. We provided formalin-inactivated SARS-CoV-2 and confirmed RNA copy numbers. In addition, the virus genome was confirmed with whole-genome sequencing and identified as Wuhan/WI04/2019. Seven laboratories were invited for this collaborative study, according to the reporting data, we determined the SARS-CoV-2 with the unit of 6.35 Log(10) copies/mL as the national standard. The availability of the national standard (NS) of SARS-CoV-2 will facilitate the standardization and harmonization of SARS-CoV-2 NAAT assays.
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spelling pubmed-94286002022-08-31 A collaborative study to establish the national standard for SARS-CoV-2 RNA nucleic acid amplification techniques (NAAT) in Taiwan Lin, Po-Lin Wu, Ming-Sian Wu, Po-Chi Chen, Hsin-Mei Peng, Yi-Hsuan Hsu, Jia-Chuan Wang, Der-Yuan Biologicals Article The conventional PCR remains a valuable method to detect the newly emergent coronavirus rapidly and accurately. Our investigation aimed to establish the standard materials of SARS-CoV-2 for NAAT detection. We provided formalin-inactivated SARS-CoV-2 and confirmed RNA copy numbers. In addition, the virus genome was confirmed with whole-genome sequencing and identified as Wuhan/WI04/2019. Seven laboratories were invited for this collaborative study, according to the reporting data, we determined the SARS-CoV-2 with the unit of 6.35 Log(10) copies/mL as the national standard. The availability of the national standard (NS) of SARS-CoV-2 will facilitate the standardization and harmonization of SARS-CoV-2 NAAT assays. International Alliance for Biological Standardization. Published by Elsevier Ltd. 2022-09 2022-08-31 /pmc/articles/PMC9428600/ /pubmed/36085128 http://dx.doi.org/10.1016/j.biologicals.2022.08.002 Text en © 2022 International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Lin, Po-Lin
Wu, Ming-Sian
Wu, Po-Chi
Chen, Hsin-Mei
Peng, Yi-Hsuan
Hsu, Jia-Chuan
Wang, Der-Yuan
A collaborative study to establish the national standard for SARS-CoV-2 RNA nucleic acid amplification techniques (NAAT) in Taiwan
title A collaborative study to establish the national standard for SARS-CoV-2 RNA nucleic acid amplification techniques (NAAT) in Taiwan
title_full A collaborative study to establish the national standard for SARS-CoV-2 RNA nucleic acid amplification techniques (NAAT) in Taiwan
title_fullStr A collaborative study to establish the national standard for SARS-CoV-2 RNA nucleic acid amplification techniques (NAAT) in Taiwan
title_full_unstemmed A collaborative study to establish the national standard for SARS-CoV-2 RNA nucleic acid amplification techniques (NAAT) in Taiwan
title_short A collaborative study to establish the national standard for SARS-CoV-2 RNA nucleic acid amplification techniques (NAAT) in Taiwan
title_sort collaborative study to establish the national standard for sars-cov-2 rna nucleic acid amplification techniques (naat) in taiwan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9428600/
https://www.ncbi.nlm.nih.gov/pubmed/36085128
http://dx.doi.org/10.1016/j.biologicals.2022.08.002
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