Generation of dual-gRNA library for combinatorial CRISPR screening of synthetic lethal gene pairs

Combinatorial CRISPR screening is useful for investigating synthetic lethality (SL) gene pairs. Here, we detail the steps for dual-gRNA library construction, with the introduction of two backbones, LentiGuide_DKO and LentiCRISPR_DKO. We describe steps for in vitro screening with 22Rv1-Cas9 and SaOS2...

Descripción completa

Detalles Bibliográficos
Autores principales: Tang, Shan, Wu, Xue, Liu, Jinghui, Zhang, Qiongsi, Wang, Xinyi, Shao, Shuai, Gokbag, Birkan, Fan, Kunjie, Liu, Xiaoqi, Li, Fuhai, Cheng, Lijun, Li, Lang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9428847/
https://www.ncbi.nlm.nih.gov/pubmed/36060092
http://dx.doi.org/10.1016/j.xpro.2022.101556

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9428847/
https://www.ncbi.nlm.nih.gov/pubmed/36060092
http://dx.doi.org/10.1016/j.xpro.2022.101556

Ejemplares similares