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Surface Motility Favors Codependent Interaction between Pseudomonas aeruginosa and Burkholderia cenocepacia

Interactions between different bacterial species shape bacterial communities and their environments. The opportunistic pathogens Pseudomonas aeruginosa and Burkholderia cenocepacia both can colonize the lungs of individuals affected by cystic fibrosis. Using the social surface behavior called swarmi...

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Autores principales: Morin, Charles, Landry, May, Groleau, Marie-Christine, Déziel, Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9429929/
https://www.ncbi.nlm.nih.gov/pubmed/35862793
http://dx.doi.org/10.1128/msphere.00153-22
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author Morin, Charles
Landry, May
Groleau, Marie-Christine
Déziel, Eric
author_facet Morin, Charles
Landry, May
Groleau, Marie-Christine
Déziel, Eric
author_sort Morin, Charles
collection PubMed
description Interactions between different bacterial species shape bacterial communities and their environments. The opportunistic pathogens Pseudomonas aeruginosa and Burkholderia cenocepacia both can colonize the lungs of individuals affected by cystic fibrosis. Using the social surface behavior called swarming motility as a study model, we noticed intricate interactions between B. cenocepacia K56-2 and P. aeruginosa PA14. While strain K56-2 does not swarm under P. aeruginosa favorable swarming conditions, co-inoculation with a nonmotile PA14 flagellum-less ΔfliC mutant restored spreading for both strains. We show that P. aeruginosa provides the wetting agent rhamnolipids allowing K56-2 to perform swarming motility, while aflagellated PA14 appears to “hitchhike” along with K56-2 cells in the swarming colony. IMPORTANCE Pseudomonas aeruginosa and Burkholderia cenocepacia are important opportunistic pathogens often found together in the airways of persons with cystic fibrosis. Laboratory cocultures of both species often ends with one taking over the other. We used a surface motility assay to study the social interactions between populations of these bacterial species. Under our conditions, B. cenocepacia cannot swarm without supplementation of the wetting agent produced by P. aeruginosa. In a mixed colony of both species, an aflagellated mutant of P. aeruginosa provides the necessary wetting agent to B. cenocepacia, allowing both bacteria to swarm and colonize a surface. We highlight this peculiar interaction where both bacteria set aside their antagonistic tendencies to travel together.
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spelling pubmed-94299292022-09-01 Surface Motility Favors Codependent Interaction between Pseudomonas aeruginosa and Burkholderia cenocepacia Morin, Charles Landry, May Groleau, Marie-Christine Déziel, Eric mSphere Research Article Interactions between different bacterial species shape bacterial communities and their environments. The opportunistic pathogens Pseudomonas aeruginosa and Burkholderia cenocepacia both can colonize the lungs of individuals affected by cystic fibrosis. Using the social surface behavior called swarming motility as a study model, we noticed intricate interactions between B. cenocepacia K56-2 and P. aeruginosa PA14. While strain K56-2 does not swarm under P. aeruginosa favorable swarming conditions, co-inoculation with a nonmotile PA14 flagellum-less ΔfliC mutant restored spreading for both strains. We show that P. aeruginosa provides the wetting agent rhamnolipids allowing K56-2 to perform swarming motility, while aflagellated PA14 appears to “hitchhike” along with K56-2 cells in the swarming colony. IMPORTANCE Pseudomonas aeruginosa and Burkholderia cenocepacia are important opportunistic pathogens often found together in the airways of persons with cystic fibrosis. Laboratory cocultures of both species often ends with one taking over the other. We used a surface motility assay to study the social interactions between populations of these bacterial species. Under our conditions, B. cenocepacia cannot swarm without supplementation of the wetting agent produced by P. aeruginosa. In a mixed colony of both species, an aflagellated mutant of P. aeruginosa provides the necessary wetting agent to B. cenocepacia, allowing both bacteria to swarm and colonize a surface. We highlight this peculiar interaction where both bacteria set aside their antagonistic tendencies to travel together. American Society for Microbiology 2022-07-07 /pmc/articles/PMC9429929/ /pubmed/35862793 http://dx.doi.org/10.1128/msphere.00153-22 Text en Copyright © 2022 Morin et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Morin, Charles
Landry, May
Groleau, Marie-Christine
Déziel, Eric
Surface Motility Favors Codependent Interaction between Pseudomonas aeruginosa and Burkholderia cenocepacia
title Surface Motility Favors Codependent Interaction between Pseudomonas aeruginosa and Burkholderia cenocepacia
title_full Surface Motility Favors Codependent Interaction between Pseudomonas aeruginosa and Burkholderia cenocepacia
title_fullStr Surface Motility Favors Codependent Interaction between Pseudomonas aeruginosa and Burkholderia cenocepacia
title_full_unstemmed Surface Motility Favors Codependent Interaction between Pseudomonas aeruginosa and Burkholderia cenocepacia
title_short Surface Motility Favors Codependent Interaction between Pseudomonas aeruginosa and Burkholderia cenocepacia
title_sort surface motility favors codependent interaction between pseudomonas aeruginosa and burkholderia cenocepacia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9429929/
https://www.ncbi.nlm.nih.gov/pubmed/35862793
http://dx.doi.org/10.1128/msphere.00153-22
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