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Ecological Observations Based on Functional Gene Sequencing Are Sensitive to the Amplicon Processing Method

Until recently, the de facto method for short-read-based amplicon reconstruction was a sequence similarity threshold approach (operational taxonomic units [OTUs]). This has changed with the amplicon sequence variant (ASV) method where distributions are fitted to abundance profiles of individual gene...

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Detalles Bibliográficos
Autores principales: Cholet, Fabien, Lisik, Agata, Agogué, Hélène, Ijaz, Umer Z., Pineau, Philippe, Lachaussée, Nicolas, Smith, Cindy J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9429940/
https://www.ncbi.nlm.nih.gov/pubmed/35938727
http://dx.doi.org/10.1128/msphere.00324-22

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