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Performance of Xpert HIV-1 viral load test in Senegal: a country of high circulation of CRF02_AG
INTRODUCTION: the introduction of the point-of-care in HIV-1 viral load quantification appears to be a complementary strategy to the existing conventional system of the acceleration plan for the achievement of the three 90s in Senegal. The objective of this study was to evaluate the performance of t...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The African Field Epidemiology Network
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9429987/ https://www.ncbi.nlm.nih.gov/pubmed/36060855 http://dx.doi.org/10.11604/pamj.2022.42.136.32041 |
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author | Sène, Pauline Yacine Diop-Ndiaye, Halimatou Ndoye, Aissatou Sow Kiernan, Brianan Coulibaly, Khadidiatou Ndiaye, Ousseynou Diallo, Sada Ndiaye, Anna Julienne Selbe Fall, Mengue Ba, Adjiratou Aissatou Camara, Makhtar Boye, Cheikh Saad Bouh Ngom, Ndeye Fatou Lejeune, Charlotte Ndour, Cheikh Tidiane Kane, Coumba Toure |
author_facet | Sène, Pauline Yacine Diop-Ndiaye, Halimatou Ndoye, Aissatou Sow Kiernan, Brianan Coulibaly, Khadidiatou Ndiaye, Ousseynou Diallo, Sada Ndiaye, Anna Julienne Selbe Fall, Mengue Ba, Adjiratou Aissatou Camara, Makhtar Boye, Cheikh Saad Bouh Ngom, Ndeye Fatou Lejeune, Charlotte Ndour, Cheikh Tidiane Kane, Coumba Toure |
author_sort | Sène, Pauline Yacine |
collection | PubMed |
description | INTRODUCTION: the introduction of the point-of-care in HIV-1 viral load quantification appears to be a complementary strategy to the existing conventional system of the acceleration plan for the achievement of the three 90s in Senegal. The objective of this study was to evaluate the performance of the Xpert® HIV-1 viral load in the context of circulation of non-B, non-C subtypes. METHODS: two hundred samples, were tested on Xpert® HIV-1 Viral Load using 1 ml of plasma in comparison to 600 μl on Abbott Real-time HIV-1 assay. The difference between viral load values was considered significant for Dlog <0.5 log copies/ml. RESULTS: a good correlation (r=0.985) was noted and confirmed using passing-bablok regression (slope 1.048; 95% CI: 1.036 to 1.069) for 188 samples with samples. A mean difference of 0.0075 log10 copies/ml for a 95% confidence interval (CI) of 0.002 log10 copies/ml to 0.013 log10 copies/ml was obtained. Sensitivity and specificity were respectively 93.6% and 93.5% at the threshold of 1.6 log10 copies/ml and 100% and 99% at the threshold of 3.0 log10 copies/ml. CONCLUSION: these results show that Xpert® HIV-1 Viral Load has excellent performance. In Senegal, and can be used for HIV viral load monitoring. |
format | Online Article Text |
id | pubmed-9429987 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The African Field Epidemiology Network |
record_format | MEDLINE/PubMed |
spelling | pubmed-94299872022-09-01 Performance of Xpert HIV-1 viral load test in Senegal: a country of high circulation of CRF02_AG Sène, Pauline Yacine Diop-Ndiaye, Halimatou Ndoye, Aissatou Sow Kiernan, Brianan Coulibaly, Khadidiatou Ndiaye, Ousseynou Diallo, Sada Ndiaye, Anna Julienne Selbe Fall, Mengue Ba, Adjiratou Aissatou Camara, Makhtar Boye, Cheikh Saad Bouh Ngom, Ndeye Fatou Lejeune, Charlotte Ndour, Cheikh Tidiane Kane, Coumba Toure Pan Afr Med J Project Evaluation INTRODUCTION: the introduction of the point-of-care in HIV-1 viral load quantification appears to be a complementary strategy to the existing conventional system of the acceleration plan for the achievement of the three 90s in Senegal. The objective of this study was to evaluate the performance of the Xpert® HIV-1 viral load in the context of circulation of non-B, non-C subtypes. METHODS: two hundred samples, were tested on Xpert® HIV-1 Viral Load using 1 ml of plasma in comparison to 600 μl on Abbott Real-time HIV-1 assay. The difference between viral load values was considered significant for Dlog <0.5 log copies/ml. RESULTS: a good correlation (r=0.985) was noted and confirmed using passing-bablok regression (slope 1.048; 95% CI: 1.036 to 1.069) for 188 samples with samples. A mean difference of 0.0075 log10 copies/ml for a 95% confidence interval (CI) of 0.002 log10 copies/ml to 0.013 log10 copies/ml was obtained. Sensitivity and specificity were respectively 93.6% and 93.5% at the threshold of 1.6 log10 copies/ml and 100% and 99% at the threshold of 3.0 log10 copies/ml. CONCLUSION: these results show that Xpert® HIV-1 Viral Load has excellent performance. In Senegal, and can be used for HIV viral load monitoring. The African Field Epidemiology Network 2022-06-20 /pmc/articles/PMC9429987/ /pubmed/36060855 http://dx.doi.org/10.11604/pamj.2022.42.136.32041 Text en Copyright: Pauline Yacine Sène et al. https://creativecommons.org/licenses/by/4.0/The Pan African Medical Journal (ISSN: 1937-8688). This is an Open Access article distributed under the terms of the Creative Commons Attribution International 4.0 License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Project Evaluation Sène, Pauline Yacine Diop-Ndiaye, Halimatou Ndoye, Aissatou Sow Kiernan, Brianan Coulibaly, Khadidiatou Ndiaye, Ousseynou Diallo, Sada Ndiaye, Anna Julienne Selbe Fall, Mengue Ba, Adjiratou Aissatou Camara, Makhtar Boye, Cheikh Saad Bouh Ngom, Ndeye Fatou Lejeune, Charlotte Ndour, Cheikh Tidiane Kane, Coumba Toure Performance of Xpert HIV-1 viral load test in Senegal: a country of high circulation of CRF02_AG |
title | Performance of Xpert HIV-1 viral load test in Senegal: a country of high circulation of CRF02_AG |
title_full | Performance of Xpert HIV-1 viral load test in Senegal: a country of high circulation of CRF02_AG |
title_fullStr | Performance of Xpert HIV-1 viral load test in Senegal: a country of high circulation of CRF02_AG |
title_full_unstemmed | Performance of Xpert HIV-1 viral load test in Senegal: a country of high circulation of CRF02_AG |
title_short | Performance of Xpert HIV-1 viral load test in Senegal: a country of high circulation of CRF02_AG |
title_sort | performance of xpert hiv-1 viral load test in senegal: a country of high circulation of crf02_ag |
topic | Project Evaluation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9429987/ https://www.ncbi.nlm.nih.gov/pubmed/36060855 http://dx.doi.org/10.11604/pamj.2022.42.136.32041 |
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