Cargando…

Construction of a Porcine Reproductive and Respiratory Syndrome Virus with Nanoluc Luciferase Reporter: a Stable and Highly Efficient Tool for Viral Quantification Both In Vitro and In Vivo

Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically important pathogens for the global pork industry, characterized for its genetic variation and unsatisfied heterological protection from vaccines. A high-throughput screening platform for developing anti-PRRSV...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wang, Yang, Ge, Xinna, Zhang, Yongning, Guo, Xin, Han, Jun, Zhou, Lei, Yang, Hanchun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Microbiology 2022
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9430303/ https://www.ncbi.nlm.nih.gov/pubmed/35758677 http://dx.doi.org/10.1128/spectrum.00276-22

Ejemplares similares

A NanoLuc Luciferase Reporter Pseudorabies Virus for Live Imaging and Quantification of Viral Infection
por: Wang, Yalin, et al.
Publicado: (2020)

Evolutionary Patterns of Codon Usage in Major Lineages of Porcine Reproductive and Respiratory Syndrome Virus in China
por: Wu, Weixin, et al.
Publicado: (2021)

Attenuation of porcine deltacoronavirus disease severity by porcine reproductive and respiratory syndrome virus coinfection in a weaning pig model
por: Zhou, Xinrong, et al.
Publicado: (2021)

A novel strategy to attenuate porcine reproductive and respiratory syndrome virus by inhibiting viral replication in the target pulmonary alveolar macrophages via hematopoietic-specific miR-142
por: Tan, Shaoyuan, et al.
Publicado: (2023)

Engineering and exploiting synthetic allostery of NanoLuc luciferase
por: Guo, Zhong, et al.
Publicado: (2022)

Illuminating the mechanism and allosteric behavior of NanoLuc luciferase
por: Nemergut, Michal, et al.
Publicado: (2023)

Porcine Reproductive and Respiratory Syndrome Modified Live Virus Vaccine: A “Leaky” Vaccine with Debatable Efficacy and Safety
por: Zhou, Lei, et al.
Publicado: (2021)

NADC30-like Strain of Porcine Reproductive and Respiratory Syndrome Virus, China
por: Zhou, Lei, et al.
Publicado: (2015)

Identification of a novel linear B-cell epitope in nonstructural protein 11 of porcine reproductive and respiratory syndrome virus that are conserved in both genotypes
por: Jiang, Nan, et al.
Publicado: (2017)

Autophagy sustains the replication of porcine reproductive and respiratory virus in host cells
por: Liu, Qinghao, et al.
Publicado: (2012)

Mapping the Key Residues within the Porcine Reproductive and Respiratory Syndrome Virus nsp1α Replicase Protein Required for Degradation of Swine Leukocyte Antigen Class I Molecules
por: Liu, Yuanyuan, et al.
Publicado: (2022)

Expression of NanoLuc Luciferase in Listeria innocua for Development of Biofilm Assay
por: Berlec, Aleš, et al.
Publicado: (2021)

Attenuated Porcine Reproductive and Respiratory Syndrome Virus Regains Its Fatal Virulence by Serial Passaging in Pigs or Porcine Alveolar Macrophages To Increase Its Adaptation to Target Cells
por: Wang, Jingjing, et al.
Publicado: (2022)

Induction of Apoptosis by the Nonstructural Protein 4 and 10 of Porcine Reproductive and Respiratory Syndrome Virus
por: Yuan, Shuaizhen, et al.
Publicado: (2016)

The DEAD-box RNA helicase 5 positively regulates the replication of porcine reproductive and respiratory syndrome virus by interacting with viral Nsp9 in vitro
por: Zhao, Shuangcheng, et al.
Publicado: (2015)

Porcine reproductive and respiratory syndrome virus suppresses post-transcriptionally the protein expression of IFN-β by upregulating cellular microRNAs in porcine alveolar macrophages in vitro
por: Wang, Lilin, et al.
Publicado: (2018)

Construction and Generation of a Recombinant Senecavirus a Stably Expressing the NanoLuc Luciferase for Quantitative Antiviral Assay
por: Guo, Xiaoran, et al.
Publicado: (2021)

NanoLuc Luciferase – A Multifunctional Tool for High Throughput Antibody Screening
por: Boute, Nicolas, et al.
Publicado: (2016)

The interaction of nonstructural protein 9 with retinoblastoma protein benefits the replication of genotype 2 porcine reproductive and respiratory syndrome virus in vitro
por: Dong, Jianguo, et al.
Publicado: (2014)

Reprogramming the unfolded protein response for replication by porcine reproductive and respiratory syndrome virus
por: Gao, Peng, et al.
Publicado: (2019)

Genetic Diversity Analysis of Genotype 2 Porcine Reproductive and Respiratory Syndrome Viruses Emerging in Recent Years in China
por: Zhou, Lei, et al.
Publicado: (2014)

Interactome Profile of the Host Cellular Proteins and the Nonstructural Protein 2 of Porcine Reproductive and Respiratory Syndrome Virus
por: Wang, Li, et al.
Publicado: (2014)

Luciferase NanoLuc as a reporter for gene expression and protein levels in Saccharomyces cerevisiae
por: Masser, Anna E., et al.
Publicado: (2016)

NanoLuc Luciferase as a Fluorogen-Activating Protein for GFP Chromophore Based Fluorogens
por: Bogdanova, Yulia A., et al.
Publicado: (2023)

Chinese highly pathogenic porcine reproductive and respiratory syndrome virus exhibits more extensive tissue tropism for pigs
por: Li, Limin, et al.
Publicado: (2012)

Interleukin-2 enhancer binding factor 2 interacts with the nsp9 or nsp2 of porcine reproductive and respiratory syndrome virus and exerts negatively regulatory effect on the viral replication
por: Wen, Xuexia, et al.
Publicado: (2017)

Transcriptome Analysis Reveals Dynamic Gene Expression Profiles in Porcine Alveolar Macrophages in Response to the Chinese Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus
por: Zeng, Nanfang, et al.
Publicado: (2018)

Interaction of porcine reproductive and respiratory syndrome virus proteins with SUMO-conjugating enzyme reveals the SUMOylation of nucleocapsid protein
por: Wang, Cong, et al.
Publicado: (2017)

Nsp9 and Nsp10 Contribute to the Fatal Virulence of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Emerging in China
por: Li, Yan, et al.
Publicado: (2014)

Recovery of NanoLuc Luciferase-Tagged Canine Distemper Virus for Facilitating Rapid Screening of Antivirals in vitro
por: Liu, Fuxiao, et al.
Publicado: (2020)

PRRSV Promotes MARC-145 Cells Entry Into S Phase of the Cell Cycle to Facilitate Viral Replication via Degradation of p21 by nsp11
por: Wen, Xuexia, et al.
Publicado: (2021)

Development of luciferase-linked antibody capture assay based on luciferase immunoprecipitation systems for antibody detection of porcine reproductive and respiratory syndrome virus
por: Li, Jie, et al.
Publicado: (2018)

Nonstructural protein 9 residues 586 and 592 are critical sites in determining the replication efficiency and fatal virulence of the Chinese highly pathogenic porcine reproductive and respiratory syndrome virus
por: Xu, Lei, et al.
Publicado: (2018)

Multicolour In Vivo Bioluminescence Imaging Using a NanoLuc‐Based BRET Reporter in Combination with Firefly Luciferase
por: Taylor, Arthur, et al.
Publicado: (2018)

Comparative Analysis of NanoLuc Luciferase and Alkaline Phosphatase Luminescence Reporter Systems for Phage-Based Detection of Bacteria
por: Wijeratne, Shalini, et al.
Publicado: (2022)

LUNAS: A Rapid and Sensitive Nucleic Acid Detection Assay Using Split NanoLuc Luciferase Complementation
por: Liu, Longwei, et al.
Publicado: (2023)

Prevalence and Evolution Analysis of Porcine Circovirus 3 in China from 2018 to 2022
por: Chen, Dengjin, et al.
Publicado: (2022)

Sensing chemical-induced genotoxicity and oxidative stress via yeast-based reporter assays using NanoLuc luciferase
por: Shichinohe, Minami, et al.
Publicado: (2023)

A nucleic acid detection assay combining reverse transcription recombinase-aided amplification with a lateral flow dipstick for the rapid visual detection of porcine deltacoronavirus
por: Zeng, Jianyu, et al.
Publicado: (2022)

Analysis of RAS and drug induced homo- and heterodimerization of RAF and KSR1 proteins in living cells using split Nanoluc luciferase
por: Rohrer, Lino, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_g6rji80arlhv1itqu47ilchmmc