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How cryoprotectants work: hydrogen-bonding in low-temperature vitrified solutions

Dimethyl sulfoxide (DMSO) increases cell and tissue viability at low temperatures and is commonly used as a cryoprotectant for cryogenic storage of biological materials. DMSO disorders the water hydrogen-bond networks and inhibits ice-crystal growth, though the specific DMSO interactions with water...

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Detalles Bibliográficos
Autores principales: Lee, Euihyun, Baiz, Carlos R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9430440/
https://www.ncbi.nlm.nih.gov/pubmed/36128234
http://dx.doi.org/10.1039/d2sc03188d
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author Lee, Euihyun
Baiz, Carlos R.
author_facet Lee, Euihyun
Baiz, Carlos R.
author_sort Lee, Euihyun
collection PubMed
description Dimethyl sulfoxide (DMSO) increases cell and tissue viability at low temperatures and is commonly used as a cryoprotectant for cryogenic storage of biological materials. DMSO disorders the water hydrogen-bond networks and inhibits ice-crystal growth, though the specific DMSO interactions with water are difficult to characterize. In this study, we use a combination of Fourier Transform infrared spectroscopy (FTIR), molecular dynamics simulations, and vibrational frequency maps to characterize the temperature-dependent hydrogen bonding interactions of DMSO with water from 30 °C to −80 °C. Specifically, broad peaks in O–D stretch vibrational spectra of DMSO and deuterated water (HDO) cosolvent systems show that the hydrogen bond networks become increasingly disrupted compared to pure water. Simulations demonstrated that these disrupted hydrogen bond networks remain largely localized to the first hydration shell of DMSO, which explains the high DMSO concentrations needed to prevent ice crystal formation in cryopreservation applications.
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spelling pubmed-94304402022-09-19 How cryoprotectants work: hydrogen-bonding in low-temperature vitrified solutions Lee, Euihyun Baiz, Carlos R. Chem Sci Chemistry Dimethyl sulfoxide (DMSO) increases cell and tissue viability at low temperatures and is commonly used as a cryoprotectant for cryogenic storage of biological materials. DMSO disorders the water hydrogen-bond networks and inhibits ice-crystal growth, though the specific DMSO interactions with water are difficult to characterize. In this study, we use a combination of Fourier Transform infrared spectroscopy (FTIR), molecular dynamics simulations, and vibrational frequency maps to characterize the temperature-dependent hydrogen bonding interactions of DMSO with water from 30 °C to −80 °C. Specifically, broad peaks in O–D stretch vibrational spectra of DMSO and deuterated water (HDO) cosolvent systems show that the hydrogen bond networks become increasingly disrupted compared to pure water. Simulations demonstrated that these disrupted hydrogen bond networks remain largely localized to the first hydration shell of DMSO, which explains the high DMSO concentrations needed to prevent ice crystal formation in cryopreservation applications. The Royal Society of Chemistry 2022-08-08 /pmc/articles/PMC9430440/ /pubmed/36128234 http://dx.doi.org/10.1039/d2sc03188d Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Lee, Euihyun
Baiz, Carlos R.
How cryoprotectants work: hydrogen-bonding in low-temperature vitrified solutions
title How cryoprotectants work: hydrogen-bonding in low-temperature vitrified solutions
title_full How cryoprotectants work: hydrogen-bonding in low-temperature vitrified solutions
title_fullStr How cryoprotectants work: hydrogen-bonding in low-temperature vitrified solutions
title_full_unstemmed How cryoprotectants work: hydrogen-bonding in low-temperature vitrified solutions
title_short How cryoprotectants work: hydrogen-bonding in low-temperature vitrified solutions
title_sort how cryoprotectants work: hydrogen-bonding in low-temperature vitrified solutions
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9430440/
https://www.ncbi.nlm.nih.gov/pubmed/36128234
http://dx.doi.org/10.1039/d2sc03188d
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