Culture-Free Phylogenetic Analysis of Legionella pneumophila Using Targeted CRISPR/Cas9 Next-Generation Sequencing

Currently available methods for the laboratory investigation of Legionella pneumophila outbreaks require organism culture. The ability to sequence L. pneumophila directly from clinical samples would significantly reduce delays. Here, we develop a method for targeted next-generation sequencing (NGS)...

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Detalles Bibliográficos
Autores principales: Domazetovska, Ana, Jensen, Slade O., Gray, Matthew, Radzieta, Michael, Maley, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9430934/
https://www.ncbi.nlm.nih.gov/pubmed/35862996
http://dx.doi.org/10.1128/spectrum.00359-22

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