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Seeing the long tail: A novel green fluorescent protein, SiriusGFP, for ultra long timelapse imaging
BACKGROUND: Fluorescent proteins (FPs) have widespread uses in cell biology. However, the practical applications of FPs are significantly limited due to their rapid photobleaching and misfolding when fused to target proteins. NEW METHOD: Using a combination of novel and known mutations to eGFP, we d...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9431725/ https://www.ncbi.nlm.nih.gov/pubmed/30578868 http://dx.doi.org/10.1016/j.jneumeth.2018.12.008 |