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Chloroquine alleviates the heat-induced to injure via autophagy and apoptosis mechanisms in skin cell and mouse models

Burns can cause cell death and irreversible tissue damage. We examined the pathway of human dermis fibroblasts cell death caused by skin burns and the roles of chloroquine in human skin keratinocytes HaCaT wound healing. Western blot assays were performed to assess expression of proteins associated...

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Autores principales: Tang, Sheau-Chung, Ko, Jiunn-Liang, Lu, Chun-Te, Leong, Pui-Ying, Ou, Chu-Chyn, Hsu, Chih-Ting, Hsiao, Yu-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9432730/
https://www.ncbi.nlm.nih.gov/pubmed/36044415
http://dx.doi.org/10.1371/journal.pone.0272797
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author Tang, Sheau-Chung
Ko, Jiunn-Liang
Lu, Chun-Te
Leong, Pui-Ying
Ou, Chu-Chyn
Hsu, Chih-Ting
Hsiao, Yu-Ping
author_facet Tang, Sheau-Chung
Ko, Jiunn-Liang
Lu, Chun-Te
Leong, Pui-Ying
Ou, Chu-Chyn
Hsu, Chih-Ting
Hsiao, Yu-Ping
author_sort Tang, Sheau-Chung
collection PubMed
description Burns can cause cell death and irreversible tissue damage. We examined the pathway of human dermis fibroblasts cell death caused by skin burns and the roles of chloroquine in human skin keratinocytes HaCaT wound healing. Western blot assays were performed to assess expression of proteins associated with autophagy, apoptosis, and endoplasmic reticulum stress in skin cells following burns. Changes in apoptosis-related proteins were assessed using flow cytometry, and wound cell migration was examined using wound healing assays. The burn animal model was used to test whether chloroquine would promote wound healing. In human burned fibroblasts, expression of LC3B-II and Cleave-caspase-7 was increased, whereas expression of Beclin-1, p62, and Grp78 was decreased. Severe burn induced ER stress and ERK phosphorylation, but PD98059 or necrostatin-1 treatment cells did not affect expression of autophagy LC3B-II protein and can induce apoptosis. Even though added with TGF-β and FGF did not repair autophagy caused by burns. Suggesting that autophagy and apoptosis were involved in heat-injured mechanism. Recombinant Wnt3a protein can help restore expression of β-catenin which reduced following burns in keratinocytes. Wnt3a protein can promote migration of keratinocytes after burns. Interesting, chloroquine increased expression of LC3B-II protein and restored cell migration activity after 24 h of burns. Consistently, surgical dressing containing chloroquine promoted wound healing in a burn animal mode. Autophagy and Wnt/β-catenin is two signalling pathways that participate in cell repair and wound healing in human fibroblasts, keratinocytes. Surgical dressing containing chloroquine can recover wound healing in burned rats.
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spelling pubmed-94327302022-09-01 Chloroquine alleviates the heat-induced to injure via autophagy and apoptosis mechanisms in skin cell and mouse models Tang, Sheau-Chung Ko, Jiunn-Liang Lu, Chun-Te Leong, Pui-Ying Ou, Chu-Chyn Hsu, Chih-Ting Hsiao, Yu-Ping PLoS One Research Article Burns can cause cell death and irreversible tissue damage. We examined the pathway of human dermis fibroblasts cell death caused by skin burns and the roles of chloroquine in human skin keratinocytes HaCaT wound healing. Western blot assays were performed to assess expression of proteins associated with autophagy, apoptosis, and endoplasmic reticulum stress in skin cells following burns. Changes in apoptosis-related proteins were assessed using flow cytometry, and wound cell migration was examined using wound healing assays. The burn animal model was used to test whether chloroquine would promote wound healing. In human burned fibroblasts, expression of LC3B-II and Cleave-caspase-7 was increased, whereas expression of Beclin-1, p62, and Grp78 was decreased. Severe burn induced ER stress and ERK phosphorylation, but PD98059 or necrostatin-1 treatment cells did not affect expression of autophagy LC3B-II protein and can induce apoptosis. Even though added with TGF-β and FGF did not repair autophagy caused by burns. Suggesting that autophagy and apoptosis were involved in heat-injured mechanism. Recombinant Wnt3a protein can help restore expression of β-catenin which reduced following burns in keratinocytes. Wnt3a protein can promote migration of keratinocytes after burns. Interesting, chloroquine increased expression of LC3B-II protein and restored cell migration activity after 24 h of burns. Consistently, surgical dressing containing chloroquine promoted wound healing in a burn animal mode. Autophagy and Wnt/β-catenin is two signalling pathways that participate in cell repair and wound healing in human fibroblasts, keratinocytes. Surgical dressing containing chloroquine can recover wound healing in burned rats. Public Library of Science 2022-08-31 /pmc/articles/PMC9432730/ /pubmed/36044415 http://dx.doi.org/10.1371/journal.pone.0272797 Text en © 2022 Tang et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Tang, Sheau-Chung
Ko, Jiunn-Liang
Lu, Chun-Te
Leong, Pui-Ying
Ou, Chu-Chyn
Hsu, Chih-Ting
Hsiao, Yu-Ping
Chloroquine alleviates the heat-induced to injure via autophagy and apoptosis mechanisms in skin cell and mouse models
title Chloroquine alleviates the heat-induced to injure via autophagy and apoptosis mechanisms in skin cell and mouse models
title_full Chloroquine alleviates the heat-induced to injure via autophagy and apoptosis mechanisms in skin cell and mouse models
title_fullStr Chloroquine alleviates the heat-induced to injure via autophagy and apoptosis mechanisms in skin cell and mouse models
title_full_unstemmed Chloroquine alleviates the heat-induced to injure via autophagy and apoptosis mechanisms in skin cell and mouse models
title_short Chloroquine alleviates the heat-induced to injure via autophagy and apoptosis mechanisms in skin cell and mouse models
title_sort chloroquine alleviates the heat-induced to injure via autophagy and apoptosis mechanisms in skin cell and mouse models
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9432730/
https://www.ncbi.nlm.nih.gov/pubmed/36044415
http://dx.doi.org/10.1371/journal.pone.0272797
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