Base editor enables rational genome-scale functional screening for enhanced industrial phenotypes in Corynebacterium glutamicum

Genome-scale functional screening accelerates comprehensive assessment of gene function in cells. Here, we have established a genome-scale loss-of-function screening strategy that combined a cytosine base editor with approximately 12,000 parallel sgRNAs targeting 98.1% of total genes in Corynebacter...

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Detalles Bibliográficos
Autores principales: Liu, Ye, Wang, Ruoyu, Liu, Jiahui, Lu, Hui, Li, Haoran, Wang, Yu, Ni, Xiaomeng, Li, Junwei, Guo, Yanmei, Ma, Hongwu, Liao, Xiaoping, Wang, Meng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2022
Materias:
Biomedicine and Life Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9432829/
https://www.ncbi.nlm.nih.gov/pubmed/36044571
http://dx.doi.org/10.1126/sciadv.abq2157

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