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Diverse states and stimuli tune olfactory receptor expression levels to modulate food-seeking behavior

Animals must weigh competing needs and states to generate adaptive behavioral responses to the environment. Sensorimotor circuits are thus tasked with integrating diverse external and internal cues relevant to these needs to generate context-appropriate behaviors. However, the mechanisms that underl...

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Autores principales: McLachlan, Ian G, Kramer, Talya S, Dua, Malvika, DiLoreto, Elizabeth M, Gomes, Matthew A, Dag, Ugur, Srinivasan, Jagan, Flavell, Steven W
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9433090/
https://www.ncbi.nlm.nih.gov/pubmed/36044259
http://dx.doi.org/10.7554/eLife.79557
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author McLachlan, Ian G
Kramer, Talya S
Dua, Malvika
DiLoreto, Elizabeth M
Gomes, Matthew A
Dag, Ugur
Srinivasan, Jagan
Flavell, Steven W
author_facet McLachlan, Ian G
Kramer, Talya S
Dua, Malvika
DiLoreto, Elizabeth M
Gomes, Matthew A
Dag, Ugur
Srinivasan, Jagan
Flavell, Steven W
author_sort McLachlan, Ian G
collection PubMed
description Animals must weigh competing needs and states to generate adaptive behavioral responses to the environment. Sensorimotor circuits are thus tasked with integrating diverse external and internal cues relevant to these needs to generate context-appropriate behaviors. However, the mechanisms that underlie this integration are largely unknown. Here, we show that a wide range of states and stimuli converge upon a single Caenorhabditis elegans olfactory neuron to modulate food-seeking behavior. Using an unbiased ribotagging approach, we find that the expression of olfactory receptor genes in the AWA olfactory neuron is influenced by a wide array of states and stimuli, including feeding state, physiological stress, and recent sensory cues. We identify odorants that activate these state-dependent olfactory receptors and show that altered expression of these receptors influences food-seeking and foraging. Further, we dissect the molecular and neural circuit pathways through which external sensory information and internal nutritional state are integrated by AWA. This reveals a modular organization in which sensory and state-related signals arising from different cell types in the body converge on AWA and independently control chemoreceptor expression. The synthesis of these signals by AWA allows animals to generate sensorimotor responses that reflect the animal’s overall state. Our findings suggest a general model in which sensory- and state-dependent transcriptional changes at the sensory periphery modulate animals’ sensorimotor responses to meet their ongoing needs and states.
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spelling pubmed-94330902022-09-01 Diverse states and stimuli tune olfactory receptor expression levels to modulate food-seeking behavior McLachlan, Ian G Kramer, Talya S Dua, Malvika DiLoreto, Elizabeth M Gomes, Matthew A Dag, Ugur Srinivasan, Jagan Flavell, Steven W eLife Neuroscience Animals must weigh competing needs and states to generate adaptive behavioral responses to the environment. Sensorimotor circuits are thus tasked with integrating diverse external and internal cues relevant to these needs to generate context-appropriate behaviors. However, the mechanisms that underlie this integration are largely unknown. Here, we show that a wide range of states and stimuli converge upon a single Caenorhabditis elegans olfactory neuron to modulate food-seeking behavior. Using an unbiased ribotagging approach, we find that the expression of olfactory receptor genes in the AWA olfactory neuron is influenced by a wide array of states and stimuli, including feeding state, physiological stress, and recent sensory cues. We identify odorants that activate these state-dependent olfactory receptors and show that altered expression of these receptors influences food-seeking and foraging. Further, we dissect the molecular and neural circuit pathways through which external sensory information and internal nutritional state are integrated by AWA. This reveals a modular organization in which sensory and state-related signals arising from different cell types in the body converge on AWA and independently control chemoreceptor expression. The synthesis of these signals by AWA allows animals to generate sensorimotor responses that reflect the animal’s overall state. Our findings suggest a general model in which sensory- and state-dependent transcriptional changes at the sensory periphery modulate animals’ sensorimotor responses to meet their ongoing needs and states. eLife Sciences Publications, Ltd 2022-08-31 /pmc/articles/PMC9433090/ /pubmed/36044259 http://dx.doi.org/10.7554/eLife.79557 Text en © 2022, McLachlan et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
McLachlan, Ian G
Kramer, Talya S
Dua, Malvika
DiLoreto, Elizabeth M
Gomes, Matthew A
Dag, Ugur
Srinivasan, Jagan
Flavell, Steven W
Diverse states and stimuli tune olfactory receptor expression levels to modulate food-seeking behavior
title Diverse states and stimuli tune olfactory receptor expression levels to modulate food-seeking behavior
title_full Diverse states and stimuli tune olfactory receptor expression levels to modulate food-seeking behavior
title_fullStr Diverse states and stimuli tune olfactory receptor expression levels to modulate food-seeking behavior
title_full_unstemmed Diverse states and stimuli tune olfactory receptor expression levels to modulate food-seeking behavior
title_short Diverse states and stimuli tune olfactory receptor expression levels to modulate food-seeking behavior
title_sort diverse states and stimuli tune olfactory receptor expression levels to modulate food-seeking behavior
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9433090/
https://www.ncbi.nlm.nih.gov/pubmed/36044259
http://dx.doi.org/10.7554/eLife.79557
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