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In vitro chemosensitivity of a canine tumor venereal transmissible cancer cell line
The canine transmissible venereal tumor (CTVT) is the most common malignity in dogs. Because there are reports that this tumor is resistant to vincristine sulfate, the chemotherapeutic options are scarce, and the development of new therapeutic approaches is necessary. In this study, we evaluated the...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9433647/ https://www.ncbi.nlm.nih.gov/pubmed/36061122 http://dx.doi.org/10.3389/fvets.2022.972185 |
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author | Franco Molina, Moisés Armides Santamaría-Martínez, Edson Antonio Santana Krimskaya, Silvia Elena Zarate-Triviño, Diana Ginette Kawas, Jorge R. Ramos Zayas, Yareellys Palacios Estrada, Natanael Prado García, Heriberto García Coronado, Paola Leonor Rodríguez Padilla, Cristina |
author_facet | Franco Molina, Moisés Armides Santamaría-Martínez, Edson Antonio Santana Krimskaya, Silvia Elena Zarate-Triviño, Diana Ginette Kawas, Jorge R. Ramos Zayas, Yareellys Palacios Estrada, Natanael Prado García, Heriberto García Coronado, Paola Leonor Rodríguez Padilla, Cristina |
author_sort | Franco Molina, Moisés Armides |
collection | PubMed |
description | The canine transmissible venereal tumor (CTVT) is the most common malignity in dogs. Because there are reports that this tumor is resistant to vincristine sulfate, the chemotherapeutic options are scarce, and the development of new therapeutic approaches is necessary. In this study, we evaluated the cytotoxic activity of vincristine, doxorubicin, temozolomide, panobinostat, toceranib, gemcitabine, cisplatin, fluorouracil, cyclophosphamide, and methotrexate on a CTVT cell line, determining that all drugs decreased the viability in a dose-dependent manner. Furthermore, they inhibit cellular migration in a time- and drug-dependent manner, as evaluated by the wound healing assay. On the other hand, vincristine, panobinostat, gemcitabine, toceranib, cyclophosphamide, and methotrexate increased the percentage of cells in the subG1 phase, and doxorubicin, temozolomide, gemcitabine, toceranib, and methotrexate decreased the percentage of cells in the synthesis phase. To efficientize the use of vincristine, only toceranib increased the cytotoxic effect of vincristine in a synergistic manner. Our results confirm the use of vincristine as the gold standard for CTVT treatment as monotherapy and suggest the use of a combinatorial and sequential treatment with toceranib. |
format | Online Article Text |
id | pubmed-9433647 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94336472022-09-02 In vitro chemosensitivity of a canine tumor venereal transmissible cancer cell line Franco Molina, Moisés Armides Santamaría-Martínez, Edson Antonio Santana Krimskaya, Silvia Elena Zarate-Triviño, Diana Ginette Kawas, Jorge R. Ramos Zayas, Yareellys Palacios Estrada, Natanael Prado García, Heriberto García Coronado, Paola Leonor Rodríguez Padilla, Cristina Front Vet Sci Veterinary Science The canine transmissible venereal tumor (CTVT) is the most common malignity in dogs. Because there are reports that this tumor is resistant to vincristine sulfate, the chemotherapeutic options are scarce, and the development of new therapeutic approaches is necessary. In this study, we evaluated the cytotoxic activity of vincristine, doxorubicin, temozolomide, panobinostat, toceranib, gemcitabine, cisplatin, fluorouracil, cyclophosphamide, and methotrexate on a CTVT cell line, determining that all drugs decreased the viability in a dose-dependent manner. Furthermore, they inhibit cellular migration in a time- and drug-dependent manner, as evaluated by the wound healing assay. On the other hand, vincristine, panobinostat, gemcitabine, toceranib, cyclophosphamide, and methotrexate increased the percentage of cells in the subG1 phase, and doxorubicin, temozolomide, gemcitabine, toceranib, and methotrexate decreased the percentage of cells in the synthesis phase. To efficientize the use of vincristine, only toceranib increased the cytotoxic effect of vincristine in a synergistic manner. Our results confirm the use of vincristine as the gold standard for CTVT treatment as monotherapy and suggest the use of a combinatorial and sequential treatment with toceranib. Frontiers Media S.A. 2022-08-18 /pmc/articles/PMC9433647/ /pubmed/36061122 http://dx.doi.org/10.3389/fvets.2022.972185 Text en Copyright © 2022 Franco Molina, Santamaría-Martínez, Santana Krimskaya, Zarate-Triviño, Kawas, Ramos Zayas, Palacios Estrada, Prado García, García Coronado and Rodríguez Padilla. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Franco Molina, Moisés Armides Santamaría-Martínez, Edson Antonio Santana Krimskaya, Silvia Elena Zarate-Triviño, Diana Ginette Kawas, Jorge R. Ramos Zayas, Yareellys Palacios Estrada, Natanael Prado García, Heriberto García Coronado, Paola Leonor Rodríguez Padilla, Cristina In vitro chemosensitivity of a canine tumor venereal transmissible cancer cell line |
title | In vitro chemosensitivity of a canine tumor venereal transmissible cancer cell line |
title_full | In vitro chemosensitivity of a canine tumor venereal transmissible cancer cell line |
title_fullStr | In vitro chemosensitivity of a canine tumor venereal transmissible cancer cell line |
title_full_unstemmed | In vitro chemosensitivity of a canine tumor venereal transmissible cancer cell line |
title_short | In vitro chemosensitivity of a canine tumor venereal transmissible cancer cell line |
title_sort | in vitro chemosensitivity of a canine tumor venereal transmissible cancer cell line |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9433647/ https://www.ncbi.nlm.nih.gov/pubmed/36061122 http://dx.doi.org/10.3389/fvets.2022.972185 |
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