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A droplet-based microfluidic approach to isolating functional bacteria from gut microbiota
Metabolic interactions within gut microbiota play a vital role in human health and disease. Targeting metabolically interacting bacteria could provide effective treatments; however, obtaining functional bacteria remains a significant challenge due to the complexity of gut microbiota. Here, we develo...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9433703/ https://www.ncbi.nlm.nih.gov/pubmed/36061857 http://dx.doi.org/10.3389/fcimb.2022.920986 |
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author | Yin, Jianan Chen, Xiuzhao Li, Xiaobo Kang, Guangbo Wang, Ping Song, Yanqing Ijaz, Umer Zeeshan Yin, Huabing Huang, He |
author_facet | Yin, Jianan Chen, Xiuzhao Li, Xiaobo Kang, Guangbo Wang, Ping Song, Yanqing Ijaz, Umer Zeeshan Yin, Huabing Huang, He |
author_sort | Yin, Jianan |
collection | PubMed |
description | Metabolic interactions within gut microbiota play a vital role in human health and disease. Targeting metabolically interacting bacteria could provide effective treatments; however, obtaining functional bacteria remains a significant challenge due to the complexity of gut microbiota. Here, we developed a facile droplet-based approach to isolate and enrich functional gut bacteria that could utilize metabolites from an engineered butyrate-producing bacteria (EBPB) of anti-obesity potential. This involves the high throughput formation of single-bacteria droplets, followed by culturing “droplets” on agar plates to form discrete single-cell colonies. This approach eliminates the need for sophisticated s instruments to sort droplets and thus allows the operation hosted in a traditional anaerobic chamber. In comparison to the traditional culture, the droplet-based approach obtained a community of substantially higher diversity and evenness. Using the conditioned plates containing metabolites from the EBPB supernatant, we obtained gut bacteria closely associated or interacting with the EBPB. These include anaerobic Lactobacillus and Bifidobacterium, which are often used as probiotics. The study illustrates the potential of our approach in the search for the associated bacteria within the gut microbiota and retrieving those yet-to-be cultured. |
format | Online Article Text |
id | pubmed-9433703 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94337032022-09-02 A droplet-based microfluidic approach to isolating functional bacteria from gut microbiota Yin, Jianan Chen, Xiuzhao Li, Xiaobo Kang, Guangbo Wang, Ping Song, Yanqing Ijaz, Umer Zeeshan Yin, Huabing Huang, He Front Cell Infect Microbiol Cellular and Infection Microbiology Metabolic interactions within gut microbiota play a vital role in human health and disease. Targeting metabolically interacting bacteria could provide effective treatments; however, obtaining functional bacteria remains a significant challenge due to the complexity of gut microbiota. Here, we developed a facile droplet-based approach to isolate and enrich functional gut bacteria that could utilize metabolites from an engineered butyrate-producing bacteria (EBPB) of anti-obesity potential. This involves the high throughput formation of single-bacteria droplets, followed by culturing “droplets” on agar plates to form discrete single-cell colonies. This approach eliminates the need for sophisticated s instruments to sort droplets and thus allows the operation hosted in a traditional anaerobic chamber. In comparison to the traditional culture, the droplet-based approach obtained a community of substantially higher diversity and evenness. Using the conditioned plates containing metabolites from the EBPB supernatant, we obtained gut bacteria closely associated or interacting with the EBPB. These include anaerobic Lactobacillus and Bifidobacterium, which are often used as probiotics. The study illustrates the potential of our approach in the search for the associated bacteria within the gut microbiota and retrieving those yet-to-be cultured. Frontiers Media S.A. 2022-08-18 /pmc/articles/PMC9433703/ /pubmed/36061857 http://dx.doi.org/10.3389/fcimb.2022.920986 Text en Copyright © 2022 Yin, Chen, Li, Kang, Wang, Song, Ijaz, Yin and Huang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Yin, Jianan Chen, Xiuzhao Li, Xiaobo Kang, Guangbo Wang, Ping Song, Yanqing Ijaz, Umer Zeeshan Yin, Huabing Huang, He A droplet-based microfluidic approach to isolating functional bacteria from gut microbiota |
title | A droplet-based microfluidic approach to isolating functional bacteria from gut microbiota |
title_full | A droplet-based microfluidic approach to isolating functional bacteria from gut microbiota |
title_fullStr | A droplet-based microfluidic approach to isolating functional bacteria from gut microbiota |
title_full_unstemmed | A droplet-based microfluidic approach to isolating functional bacteria from gut microbiota |
title_short | A droplet-based microfluidic approach to isolating functional bacteria from gut microbiota |
title_sort | droplet-based microfluidic approach to isolating functional bacteria from gut microbiota |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9433703/ https://www.ncbi.nlm.nih.gov/pubmed/36061857 http://dx.doi.org/10.3389/fcimb.2022.920986 |
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