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Understanding global changes of the mouse brain proteome after vaginal infection with HSV-2 using a label-free shotgun approach

Herpes simplex virus type 2 (HSV-2) is a common human pathogen that establishes lifelong latency in neurons of the nervous system. The number of severe central nervous system infections caused by the virus has increased recently. However, the pathogenesis of HSV-2 infection in the nervous system is...

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Autores principales: Cheng, Jishuai, Wang, Qingzhen, Hu, Yiwen, Mou, Tangwei, Wang, Jianbin, Wang, Lichun, Zhang, Ying, Wang, Tinghua, Li, Qihan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9433710/
https://www.ncbi.nlm.nih.gov/pubmed/36061859
http://dx.doi.org/10.3389/fcimb.2022.942334
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author Cheng, Jishuai
Wang, Qingzhen
Hu, Yiwen
Mou, Tangwei
Wang, Jianbin
Wang, Lichun
Zhang, Ying
Wang, Tinghua
Li, Qihan
author_facet Cheng, Jishuai
Wang, Qingzhen
Hu, Yiwen
Mou, Tangwei
Wang, Jianbin
Wang, Lichun
Zhang, Ying
Wang, Tinghua
Li, Qihan
author_sort Cheng, Jishuai
collection PubMed
description Herpes simplex virus type 2 (HSV-2) is a common human pathogen that establishes lifelong latency in neurons of the nervous system. The number of severe central nervous system infections caused by the virus has increased recently. However, the pathogenesis of HSV-2 infection in the nervous system is not fully understood. Here, we demonstrated global proteomic changes in the brain tissue in BALB/c mice vaginally infected with HSV-2. Data are available via ProteomeXchange with identifier PXD034186. A total of 249 differentially expressed proteins were identified in infected brain tissue. The GO and KEGG enrichment analysis of these proteins indicated that they were mainly involved in the regulation of synapse formation and synaptic excitability. In addition, genes affecting autophagy, the development of other neurodegenerative diseases, and signaling pathways relevant to other neurologic diseases were identified. Additional experiments, comparing the brain tissue of asymptomatic and symptomatic mice showed a differential expression of proteins involved in synapse formation and synaptic transmission. Others were involved in autophagy, addiction, and signaling pathways of other neurologic diseases. These results suggest that changes in synaptic structure and function, as well as autophagy, may be related to the development of neurologic abnormalities that follow HSV-2 infection. We also identified a protein GluN2A encoded by Grin2a was continuously expressed at high levels after infection. We propose that GluN2A may be a key molecule in the pathogenesis of HSV-2-induced neurologic diseases.
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spelling pubmed-94337102022-09-02 Understanding global changes of the mouse brain proteome after vaginal infection with HSV-2 using a label-free shotgun approach Cheng, Jishuai Wang, Qingzhen Hu, Yiwen Mou, Tangwei Wang, Jianbin Wang, Lichun Zhang, Ying Wang, Tinghua Li, Qihan Front Cell Infect Microbiol Cellular and Infection Microbiology Herpes simplex virus type 2 (HSV-2) is a common human pathogen that establishes lifelong latency in neurons of the nervous system. The number of severe central nervous system infections caused by the virus has increased recently. However, the pathogenesis of HSV-2 infection in the nervous system is not fully understood. Here, we demonstrated global proteomic changes in the brain tissue in BALB/c mice vaginally infected with HSV-2. Data are available via ProteomeXchange with identifier PXD034186. A total of 249 differentially expressed proteins were identified in infected brain tissue. The GO and KEGG enrichment analysis of these proteins indicated that they were mainly involved in the regulation of synapse formation and synaptic excitability. In addition, genes affecting autophagy, the development of other neurodegenerative diseases, and signaling pathways relevant to other neurologic diseases were identified. Additional experiments, comparing the brain tissue of asymptomatic and symptomatic mice showed a differential expression of proteins involved in synapse formation and synaptic transmission. Others were involved in autophagy, addiction, and signaling pathways of other neurologic diseases. These results suggest that changes in synaptic structure and function, as well as autophagy, may be related to the development of neurologic abnormalities that follow HSV-2 infection. We also identified a protein GluN2A encoded by Grin2a was continuously expressed at high levels after infection. We propose that GluN2A may be a key molecule in the pathogenesis of HSV-2-induced neurologic diseases. Frontiers Media S.A. 2022-08-18 /pmc/articles/PMC9433710/ /pubmed/36061859 http://dx.doi.org/10.3389/fcimb.2022.942334 Text en Copyright © 2022 Cheng, Wang, Hu, Mou, Wang, Wang, Zhang, Wang and Li https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Cheng, Jishuai
Wang, Qingzhen
Hu, Yiwen
Mou, Tangwei
Wang, Jianbin
Wang, Lichun
Zhang, Ying
Wang, Tinghua
Li, Qihan
Understanding global changes of the mouse brain proteome after vaginal infection with HSV-2 using a label-free shotgun approach
title Understanding global changes of the mouse brain proteome after vaginal infection with HSV-2 using a label-free shotgun approach
title_full Understanding global changes of the mouse brain proteome after vaginal infection with HSV-2 using a label-free shotgun approach
title_fullStr Understanding global changes of the mouse brain proteome after vaginal infection with HSV-2 using a label-free shotgun approach
title_full_unstemmed Understanding global changes of the mouse brain proteome after vaginal infection with HSV-2 using a label-free shotgun approach
title_short Understanding global changes of the mouse brain proteome after vaginal infection with HSV-2 using a label-free shotgun approach
title_sort understanding global changes of the mouse brain proteome after vaginal infection with hsv-2 using a label-free shotgun approach
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9433710/
https://www.ncbi.nlm.nih.gov/pubmed/36061859
http://dx.doi.org/10.3389/fcimb.2022.942334
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