Cargando…

Identification of potential key circular RNAs related to cognitive impairment after chronic constriction injury of the sciatic nerve

Chronic neuropathic pain is commonly accompanied by cognitive impairment. However, the underlying mechanism in the occurrence of cognitive deficits under constant nociceptive irritation remains elusive. Herein, we established a chronic neuropathic pain model by chronic constriction injury (CCI) of t...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Changliang, Gao, Rui, Tang, Yidan, Chen, Hai, Zhang, Xueying, Sun, Yalan, Zhao, Qi, Lv, Peilin, Wang, Haiyang, Ye-Lehmann, Shixin, Liu, Jin, Chen, Chan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9433970/
https://www.ncbi.nlm.nih.gov/pubmed/36061613
http://dx.doi.org/10.3389/fnins.2022.925300
_version_ 1784780754953175040
author Liu, Changliang
Gao, Rui
Tang, Yidan
Chen, Hai
Zhang, Xueying
Sun, Yalan
Zhao, Qi
Lv, Peilin
Wang, Haiyang
Ye-Lehmann, Shixin
Liu, Jin
Chen, Chan
author_facet Liu, Changliang
Gao, Rui
Tang, Yidan
Chen, Hai
Zhang, Xueying
Sun, Yalan
Zhao, Qi
Lv, Peilin
Wang, Haiyang
Ye-Lehmann, Shixin
Liu, Jin
Chen, Chan
author_sort Liu, Changliang
collection PubMed
description Chronic neuropathic pain is commonly accompanied by cognitive impairment. However, the underlying mechanism in the occurrence of cognitive deficits under constant nociceptive irritation remains elusive. Herein, we established a chronic neuropathic pain model by chronic constriction injury (CCI) of the unilateral sciatic nerve in rats. Behavioral tests indicated that CCI rats with long-term nociceptive threshold decline developed significant dysfunction of working memory and recognitive memory starting at 14 days and lasting for at least 21 days. Afterward, circRNA expression profiles in the hippocampus of CCI and sham rats were analyzed via high-throughput sequencing to explore the potential key factors associated with cognitive impairment induced by ongoing nociception, which showed 76 differentially expressed circRNAs, 39 upregulated and 37 downregulated, in the CCI group. These differentially expressed circRNA host genes were validated to be primarily associated with inflammation and apoptotic signaling pathways according to GO/KEGG analysis and the circRNA-miRNA-mRNA network, which was also confirmed through the analysis of neuroinflammation and neuronal apoptosis. Consequently, we assumed that enhanced neuroinflammation and neuronal apoptosis might act as potential regulators of cognitive impairment induced by chronic neuropathic pain. The identification of the regulatory mechanism would provide promising clinical biomarkers or therapeutic targets in the diagnostic prediction and intervention treatment of memory deficits under neuropathic pain conditions.
format Online
Article
Text
id pubmed-9433970
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-94339702022-09-02 Identification of potential key circular RNAs related to cognitive impairment after chronic constriction injury of the sciatic nerve Liu, Changliang Gao, Rui Tang, Yidan Chen, Hai Zhang, Xueying Sun, Yalan Zhao, Qi Lv, Peilin Wang, Haiyang Ye-Lehmann, Shixin Liu, Jin Chen, Chan Front Neurosci Neuroscience Chronic neuropathic pain is commonly accompanied by cognitive impairment. However, the underlying mechanism in the occurrence of cognitive deficits under constant nociceptive irritation remains elusive. Herein, we established a chronic neuropathic pain model by chronic constriction injury (CCI) of the unilateral sciatic nerve in rats. Behavioral tests indicated that CCI rats with long-term nociceptive threshold decline developed significant dysfunction of working memory and recognitive memory starting at 14 days and lasting for at least 21 days. Afterward, circRNA expression profiles in the hippocampus of CCI and sham rats were analyzed via high-throughput sequencing to explore the potential key factors associated with cognitive impairment induced by ongoing nociception, which showed 76 differentially expressed circRNAs, 39 upregulated and 37 downregulated, in the CCI group. These differentially expressed circRNA host genes were validated to be primarily associated with inflammation and apoptotic signaling pathways according to GO/KEGG analysis and the circRNA-miRNA-mRNA network, which was also confirmed through the analysis of neuroinflammation and neuronal apoptosis. Consequently, we assumed that enhanced neuroinflammation and neuronal apoptosis might act as potential regulators of cognitive impairment induced by chronic neuropathic pain. The identification of the regulatory mechanism would provide promising clinical biomarkers or therapeutic targets in the diagnostic prediction and intervention treatment of memory deficits under neuropathic pain conditions. Frontiers Media S.A. 2022-08-18 /pmc/articles/PMC9433970/ /pubmed/36061613 http://dx.doi.org/10.3389/fnins.2022.925300 Text en Copyright © 2022 Liu, Gao, Tang, Chen, Zhang, Sun, Zhao, Lv, Wang, Ye-Lehmann, Liu and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Liu, Changliang
Gao, Rui
Tang, Yidan
Chen, Hai
Zhang, Xueying
Sun, Yalan
Zhao, Qi
Lv, Peilin
Wang, Haiyang
Ye-Lehmann, Shixin
Liu, Jin
Chen, Chan
Identification of potential key circular RNAs related to cognitive impairment after chronic constriction injury of the sciatic nerve
title Identification of potential key circular RNAs related to cognitive impairment after chronic constriction injury of the sciatic nerve
title_full Identification of potential key circular RNAs related to cognitive impairment after chronic constriction injury of the sciatic nerve
title_fullStr Identification of potential key circular RNAs related to cognitive impairment after chronic constriction injury of the sciatic nerve
title_full_unstemmed Identification of potential key circular RNAs related to cognitive impairment after chronic constriction injury of the sciatic nerve
title_short Identification of potential key circular RNAs related to cognitive impairment after chronic constriction injury of the sciatic nerve
title_sort identification of potential key circular rnas related to cognitive impairment after chronic constriction injury of the sciatic nerve
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9433970/
https://www.ncbi.nlm.nih.gov/pubmed/36061613
http://dx.doi.org/10.3389/fnins.2022.925300
work_keys_str_mv AT liuchangliang identificationofpotentialkeycircularrnasrelatedtocognitiveimpairmentafterchronicconstrictioninjuryofthesciaticnerve
AT gaorui identificationofpotentialkeycircularrnasrelatedtocognitiveimpairmentafterchronicconstrictioninjuryofthesciaticnerve
AT tangyidan identificationofpotentialkeycircularrnasrelatedtocognitiveimpairmentafterchronicconstrictioninjuryofthesciaticnerve
AT chenhai identificationofpotentialkeycircularrnasrelatedtocognitiveimpairmentafterchronicconstrictioninjuryofthesciaticnerve
AT zhangxueying identificationofpotentialkeycircularrnasrelatedtocognitiveimpairmentafterchronicconstrictioninjuryofthesciaticnerve
AT sunyalan identificationofpotentialkeycircularrnasrelatedtocognitiveimpairmentafterchronicconstrictioninjuryofthesciaticnerve
AT zhaoqi identificationofpotentialkeycircularrnasrelatedtocognitiveimpairmentafterchronicconstrictioninjuryofthesciaticnerve
AT lvpeilin identificationofpotentialkeycircularrnasrelatedtocognitiveimpairmentafterchronicconstrictioninjuryofthesciaticnerve
AT wanghaiyang identificationofpotentialkeycircularrnasrelatedtocognitiveimpairmentafterchronicconstrictioninjuryofthesciaticnerve
AT yelehmannshixin identificationofpotentialkeycircularrnasrelatedtocognitiveimpairmentafterchronicconstrictioninjuryofthesciaticnerve
AT liujin identificationofpotentialkeycircularrnasrelatedtocognitiveimpairmentafterchronicconstrictioninjuryofthesciaticnerve
AT chenchan identificationofpotentialkeycircularrnasrelatedtocognitiveimpairmentafterchronicconstrictioninjuryofthesciaticnerve