Cargando…
Identification of potential key circular RNAs related to cognitive impairment after chronic constriction injury of the sciatic nerve
Chronic neuropathic pain is commonly accompanied by cognitive impairment. However, the underlying mechanism in the occurrence of cognitive deficits under constant nociceptive irritation remains elusive. Herein, we established a chronic neuropathic pain model by chronic constriction injury (CCI) of t...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9433970/ https://www.ncbi.nlm.nih.gov/pubmed/36061613 http://dx.doi.org/10.3389/fnins.2022.925300 |
_version_ | 1784780754953175040 |
---|---|
author | Liu, Changliang Gao, Rui Tang, Yidan Chen, Hai Zhang, Xueying Sun, Yalan Zhao, Qi Lv, Peilin Wang, Haiyang Ye-Lehmann, Shixin Liu, Jin Chen, Chan |
author_facet | Liu, Changliang Gao, Rui Tang, Yidan Chen, Hai Zhang, Xueying Sun, Yalan Zhao, Qi Lv, Peilin Wang, Haiyang Ye-Lehmann, Shixin Liu, Jin Chen, Chan |
author_sort | Liu, Changliang |
collection | PubMed |
description | Chronic neuropathic pain is commonly accompanied by cognitive impairment. However, the underlying mechanism in the occurrence of cognitive deficits under constant nociceptive irritation remains elusive. Herein, we established a chronic neuropathic pain model by chronic constriction injury (CCI) of the unilateral sciatic nerve in rats. Behavioral tests indicated that CCI rats with long-term nociceptive threshold decline developed significant dysfunction of working memory and recognitive memory starting at 14 days and lasting for at least 21 days. Afterward, circRNA expression profiles in the hippocampus of CCI and sham rats were analyzed via high-throughput sequencing to explore the potential key factors associated with cognitive impairment induced by ongoing nociception, which showed 76 differentially expressed circRNAs, 39 upregulated and 37 downregulated, in the CCI group. These differentially expressed circRNA host genes were validated to be primarily associated with inflammation and apoptotic signaling pathways according to GO/KEGG analysis and the circRNA-miRNA-mRNA network, which was also confirmed through the analysis of neuroinflammation and neuronal apoptosis. Consequently, we assumed that enhanced neuroinflammation and neuronal apoptosis might act as potential regulators of cognitive impairment induced by chronic neuropathic pain. The identification of the regulatory mechanism would provide promising clinical biomarkers or therapeutic targets in the diagnostic prediction and intervention treatment of memory deficits under neuropathic pain conditions. |
format | Online Article Text |
id | pubmed-9433970 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94339702022-09-02 Identification of potential key circular RNAs related to cognitive impairment after chronic constriction injury of the sciatic nerve Liu, Changliang Gao, Rui Tang, Yidan Chen, Hai Zhang, Xueying Sun, Yalan Zhao, Qi Lv, Peilin Wang, Haiyang Ye-Lehmann, Shixin Liu, Jin Chen, Chan Front Neurosci Neuroscience Chronic neuropathic pain is commonly accompanied by cognitive impairment. However, the underlying mechanism in the occurrence of cognitive deficits under constant nociceptive irritation remains elusive. Herein, we established a chronic neuropathic pain model by chronic constriction injury (CCI) of the unilateral sciatic nerve in rats. Behavioral tests indicated that CCI rats with long-term nociceptive threshold decline developed significant dysfunction of working memory and recognitive memory starting at 14 days and lasting for at least 21 days. Afterward, circRNA expression profiles in the hippocampus of CCI and sham rats were analyzed via high-throughput sequencing to explore the potential key factors associated with cognitive impairment induced by ongoing nociception, which showed 76 differentially expressed circRNAs, 39 upregulated and 37 downregulated, in the CCI group. These differentially expressed circRNA host genes were validated to be primarily associated with inflammation and apoptotic signaling pathways according to GO/KEGG analysis and the circRNA-miRNA-mRNA network, which was also confirmed through the analysis of neuroinflammation and neuronal apoptosis. Consequently, we assumed that enhanced neuroinflammation and neuronal apoptosis might act as potential regulators of cognitive impairment induced by chronic neuropathic pain. The identification of the regulatory mechanism would provide promising clinical biomarkers or therapeutic targets in the diagnostic prediction and intervention treatment of memory deficits under neuropathic pain conditions. Frontiers Media S.A. 2022-08-18 /pmc/articles/PMC9433970/ /pubmed/36061613 http://dx.doi.org/10.3389/fnins.2022.925300 Text en Copyright © 2022 Liu, Gao, Tang, Chen, Zhang, Sun, Zhao, Lv, Wang, Ye-Lehmann, Liu and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Liu, Changliang Gao, Rui Tang, Yidan Chen, Hai Zhang, Xueying Sun, Yalan Zhao, Qi Lv, Peilin Wang, Haiyang Ye-Lehmann, Shixin Liu, Jin Chen, Chan Identification of potential key circular RNAs related to cognitive impairment after chronic constriction injury of the sciatic nerve |
title | Identification of potential key circular RNAs related to cognitive impairment after chronic constriction injury of the sciatic nerve |
title_full | Identification of potential key circular RNAs related to cognitive impairment after chronic constriction injury of the sciatic nerve |
title_fullStr | Identification of potential key circular RNAs related to cognitive impairment after chronic constriction injury of the sciatic nerve |
title_full_unstemmed | Identification of potential key circular RNAs related to cognitive impairment after chronic constriction injury of the sciatic nerve |
title_short | Identification of potential key circular RNAs related to cognitive impairment after chronic constriction injury of the sciatic nerve |
title_sort | identification of potential key circular rnas related to cognitive impairment after chronic constriction injury of the sciatic nerve |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9433970/ https://www.ncbi.nlm.nih.gov/pubmed/36061613 http://dx.doi.org/10.3389/fnins.2022.925300 |
work_keys_str_mv | AT liuchangliang identificationofpotentialkeycircularrnasrelatedtocognitiveimpairmentafterchronicconstrictioninjuryofthesciaticnerve AT gaorui identificationofpotentialkeycircularrnasrelatedtocognitiveimpairmentafterchronicconstrictioninjuryofthesciaticnerve AT tangyidan identificationofpotentialkeycircularrnasrelatedtocognitiveimpairmentafterchronicconstrictioninjuryofthesciaticnerve AT chenhai identificationofpotentialkeycircularrnasrelatedtocognitiveimpairmentafterchronicconstrictioninjuryofthesciaticnerve AT zhangxueying identificationofpotentialkeycircularrnasrelatedtocognitiveimpairmentafterchronicconstrictioninjuryofthesciaticnerve AT sunyalan identificationofpotentialkeycircularrnasrelatedtocognitiveimpairmentafterchronicconstrictioninjuryofthesciaticnerve AT zhaoqi identificationofpotentialkeycircularrnasrelatedtocognitiveimpairmentafterchronicconstrictioninjuryofthesciaticnerve AT lvpeilin identificationofpotentialkeycircularrnasrelatedtocognitiveimpairmentafterchronicconstrictioninjuryofthesciaticnerve AT wanghaiyang identificationofpotentialkeycircularrnasrelatedtocognitiveimpairmentafterchronicconstrictioninjuryofthesciaticnerve AT yelehmannshixin identificationofpotentialkeycircularrnasrelatedtocognitiveimpairmentafterchronicconstrictioninjuryofthesciaticnerve AT liujin identificationofpotentialkeycircularrnasrelatedtocognitiveimpairmentafterchronicconstrictioninjuryofthesciaticnerve AT chenchan identificationofpotentialkeycircularrnasrelatedtocognitiveimpairmentafterchronicconstrictioninjuryofthesciaticnerve |