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Neonatal Streptococcus pneumoniae infection induces long-lasting dysbiosis of the gut microbiota in a mouse model

Early life is a “critical window” for gut microbiota development, antibiotic use during this period exerts a profound effect on gut microbial dysbiosis and asthma. In clinical practice, antibiotics are usually used in patients with bacterial infections, we previously showed that neonatal S. pneumoni...

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Autores principales: Li, Yuanyuan, Xu, Ximing, Guo, Ziyao, Li, Qinyuan, Wang, Yiying, Jian, Ding, Zhang, Guangli, Tian, Xiaoyin, Chen, Shiyi, Luo, Zhengxiu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9433971/
https://www.ncbi.nlm.nih.gov/pubmed/36060784
http://dx.doi.org/10.3389/fmicb.2022.961684
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author Li, Yuanyuan
Xu, Ximing
Guo, Ziyao
Li, Qinyuan
Wang, Yiying
Jian, Ding
Zhang, Guangli
Tian, Xiaoyin
Chen, Shiyi
Luo, Zhengxiu
author_facet Li, Yuanyuan
Xu, Ximing
Guo, Ziyao
Li, Qinyuan
Wang, Yiying
Jian, Ding
Zhang, Guangli
Tian, Xiaoyin
Chen, Shiyi
Luo, Zhengxiu
author_sort Li, Yuanyuan
collection PubMed
description Early life is a “critical window” for gut microbiota development, antibiotic use during this period exerts a profound effect on gut microbial dysbiosis and asthma. In clinical practice, antibiotics are usually used in patients with bacterial infections, we previously showed that neonatal S. pneumoniae pneumonia promoted adult-onset asthma in mice model, while it remains unclear whether neonatal S. pneumoniae infection have long-term effects on gut microbiota. Neonatal BALB/c mice were inoculated with 5*10(6) CFU D39 to establish non-lethal S. pneumoniae pneumonia model. At 2, 3, 8 weeks of age, feces in the cecum were prepared for 16S rRNA sequencing, lungs were collected for histopathologic and lung function analysis. S. pneumoniae-infected neonatal mice exhibited histopathologic lesions in their lungs and increased airway hyperresponsiveness, obvious alterations in alpha and beta diversities in the entire gut microbiota, and changes of the community structure during the breastfeeding period, infancy, and adulthood. Furthermore, gut microbial composition was modified after neonatal S. pneumoniae infection, with a decreased relative abundance of Lactobacillus in the breastfeeding period and infancy; in adulthood, the relative abundance of Allobaculum diminished while that of Proteobacteria was augmented. Neonatal S. pneumoniae infection induced a long-term alteration in microbial community composition.
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spelling pubmed-94339712022-09-02 Neonatal Streptococcus pneumoniae infection induces long-lasting dysbiosis of the gut microbiota in a mouse model Li, Yuanyuan Xu, Ximing Guo, Ziyao Li, Qinyuan Wang, Yiying Jian, Ding Zhang, Guangli Tian, Xiaoyin Chen, Shiyi Luo, Zhengxiu Front Microbiol Microbiology Early life is a “critical window” for gut microbiota development, antibiotic use during this period exerts a profound effect on gut microbial dysbiosis and asthma. In clinical practice, antibiotics are usually used in patients with bacterial infections, we previously showed that neonatal S. pneumoniae pneumonia promoted adult-onset asthma in mice model, while it remains unclear whether neonatal S. pneumoniae infection have long-term effects on gut microbiota. Neonatal BALB/c mice were inoculated with 5*10(6) CFU D39 to establish non-lethal S. pneumoniae pneumonia model. At 2, 3, 8 weeks of age, feces in the cecum were prepared for 16S rRNA sequencing, lungs were collected for histopathologic and lung function analysis. S. pneumoniae-infected neonatal mice exhibited histopathologic lesions in their lungs and increased airway hyperresponsiveness, obvious alterations in alpha and beta diversities in the entire gut microbiota, and changes of the community structure during the breastfeeding period, infancy, and adulthood. Furthermore, gut microbial composition was modified after neonatal S. pneumoniae infection, with a decreased relative abundance of Lactobacillus in the breastfeeding period and infancy; in adulthood, the relative abundance of Allobaculum diminished while that of Proteobacteria was augmented. Neonatal S. pneumoniae infection induced a long-term alteration in microbial community composition. Frontiers Media S.A. 2022-08-18 /pmc/articles/PMC9433971/ /pubmed/36060784 http://dx.doi.org/10.3389/fmicb.2022.961684 Text en Copyright © 2022 Li, Xu, Guo, Li, Wang, Jian, Zhang, Tian, Chen and Luo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Li, Yuanyuan
Xu, Ximing
Guo, Ziyao
Li, Qinyuan
Wang, Yiying
Jian, Ding
Zhang, Guangli
Tian, Xiaoyin
Chen, Shiyi
Luo, Zhengxiu
Neonatal Streptococcus pneumoniae infection induces long-lasting dysbiosis of the gut microbiota in a mouse model
title Neonatal Streptococcus pneumoniae infection induces long-lasting dysbiosis of the gut microbiota in a mouse model
title_full Neonatal Streptococcus pneumoniae infection induces long-lasting dysbiosis of the gut microbiota in a mouse model
title_fullStr Neonatal Streptococcus pneumoniae infection induces long-lasting dysbiosis of the gut microbiota in a mouse model
title_full_unstemmed Neonatal Streptococcus pneumoniae infection induces long-lasting dysbiosis of the gut microbiota in a mouse model
title_short Neonatal Streptococcus pneumoniae infection induces long-lasting dysbiosis of the gut microbiota in a mouse model
title_sort neonatal streptococcus pneumoniae infection induces long-lasting dysbiosis of the gut microbiota in a mouse model
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9433971/
https://www.ncbi.nlm.nih.gov/pubmed/36060784
http://dx.doi.org/10.3389/fmicb.2022.961684
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