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Decellularization of xenografted tumors provides cell-specific in vitro 3D environment
In vitro cell culture studies are common in the cancer research field, and reliable biomimetic 3D models are needed to ensure physiological relevance. In this manuscript, we hypothesized that decellularized xenograft tumors can serve as an optimal 3D substrate to generate a top-down approach for in...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9434107/ https://www.ncbi.nlm.nih.gov/pubmed/36059712 http://dx.doi.org/10.3389/fonc.2022.956940 |
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author | Iazzolino, Gaia Mendibil, Unai Arnaiz, Blanca Ruiz-de-Angulo, Ane Azkargorta, Mikel Uribe, Kepa B. Khatami, Neda Elortza, Felix Olalde, Beatriz Gomez-Vallejo, Vanessa Llop, Jordi Abarrategi, Ander |
author_facet | Iazzolino, Gaia Mendibil, Unai Arnaiz, Blanca Ruiz-de-Angulo, Ane Azkargorta, Mikel Uribe, Kepa B. Khatami, Neda Elortza, Felix Olalde, Beatriz Gomez-Vallejo, Vanessa Llop, Jordi Abarrategi, Ander |
author_sort | Iazzolino, Gaia |
collection | PubMed |
description | In vitro cell culture studies are common in the cancer research field, and reliable biomimetic 3D models are needed to ensure physiological relevance. In this manuscript, we hypothesized that decellularized xenograft tumors can serve as an optimal 3D substrate to generate a top-down approach for in vitro tumor modeling. Multiple tumor cell lines were xenografted and the formed solid tumors were recovered for their decellularization by several techniques and further characterization by histology and proteomics techniques. Selected decellularized tumor xenograft samples were seeded with the HCC1806 human triple-negative breast cancer (TNBC) basal-like subtype cell line, and cell behavior was compared among them and with other control 2D and 3D cell culture methods. A soft treatment using Freeze-EDTA-DNAse allows proper decellularization of xenografted tumor samples. Interestingly, proteomic data show that samples decellularized from TNBC basal-like subtype xenograft models had different extracellular matrix (ECM) compositions compared to the rest of the xenograft tumors tested. The in vitro recellularization of decellularized ECM (dECM) yields tumor-type–specific cell behavior in the TNBC context. Data show that dECM derived from xenograft tumors is a feasible substrate for reseeding purposes, thereby promoting tumor-type–specific cell behavior. These data serve as a proof-of-concept for further potential generation of patient-specific in vitro research models. |
format | Online Article Text |
id | pubmed-9434107 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94341072022-09-02 Decellularization of xenografted tumors provides cell-specific in vitro 3D environment Iazzolino, Gaia Mendibil, Unai Arnaiz, Blanca Ruiz-de-Angulo, Ane Azkargorta, Mikel Uribe, Kepa B. Khatami, Neda Elortza, Felix Olalde, Beatriz Gomez-Vallejo, Vanessa Llop, Jordi Abarrategi, Ander Front Oncol Oncology In vitro cell culture studies are common in the cancer research field, and reliable biomimetic 3D models are needed to ensure physiological relevance. In this manuscript, we hypothesized that decellularized xenograft tumors can serve as an optimal 3D substrate to generate a top-down approach for in vitro tumor modeling. Multiple tumor cell lines were xenografted and the formed solid tumors were recovered for their decellularization by several techniques and further characterization by histology and proteomics techniques. Selected decellularized tumor xenograft samples were seeded with the HCC1806 human triple-negative breast cancer (TNBC) basal-like subtype cell line, and cell behavior was compared among them and with other control 2D and 3D cell culture methods. A soft treatment using Freeze-EDTA-DNAse allows proper decellularization of xenografted tumor samples. Interestingly, proteomic data show that samples decellularized from TNBC basal-like subtype xenograft models had different extracellular matrix (ECM) compositions compared to the rest of the xenograft tumors tested. The in vitro recellularization of decellularized ECM (dECM) yields tumor-type–specific cell behavior in the TNBC context. Data show that dECM derived from xenograft tumors is a feasible substrate for reseeding purposes, thereby promoting tumor-type–specific cell behavior. These data serve as a proof-of-concept for further potential generation of patient-specific in vitro research models. Frontiers Media S.A. 2022-08-18 /pmc/articles/PMC9434107/ /pubmed/36059712 http://dx.doi.org/10.3389/fonc.2022.956940 Text en Copyright © 2022 Iazzolino, Mendibil, Arnaiz, Ruiz-de-Angulo, Azkargorta, Uribe, Khatami, Elortza, Olalde, Gomez-Vallejo, Llop and Abarrategi https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Oncology Iazzolino, Gaia Mendibil, Unai Arnaiz, Blanca Ruiz-de-Angulo, Ane Azkargorta, Mikel Uribe, Kepa B. Khatami, Neda Elortza, Felix Olalde, Beatriz Gomez-Vallejo, Vanessa Llop, Jordi Abarrategi, Ander Decellularization of xenografted tumors provides cell-specific in vitro 3D environment |
title | Decellularization of xenografted tumors provides cell-specific in vitro 3D environment |
title_full | Decellularization of xenografted tumors provides cell-specific in vitro 3D environment |
title_fullStr | Decellularization of xenografted tumors provides cell-specific in vitro 3D environment |
title_full_unstemmed | Decellularization of xenografted tumors provides cell-specific in vitro 3D environment |
title_short | Decellularization of xenografted tumors provides cell-specific in vitro 3D environment |
title_sort | decellularization of xenografted tumors provides cell-specific in vitro 3d environment |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9434107/ https://www.ncbi.nlm.nih.gov/pubmed/36059712 http://dx.doi.org/10.3389/fonc.2022.956940 |
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