Cargando…

Decellularization of xenografted tumors provides cell-specific in vitro 3D environment

In vitro cell culture studies are common in the cancer research field, and reliable biomimetic 3D models are needed to ensure physiological relevance. In this manuscript, we hypothesized that decellularized xenograft tumors can serve as an optimal 3D substrate to generate a top-down approach for in...

Descripción completa

Detalles Bibliográficos
Autores principales: Iazzolino, Gaia, Mendibil, Unai, Arnaiz, Blanca, Ruiz-de-Angulo, Ane, Azkargorta, Mikel, Uribe, Kepa B., Khatami, Neda, Elortza, Felix, Olalde, Beatriz, Gomez-Vallejo, Vanessa, Llop, Jordi, Abarrategi, Ander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9434107/
https://www.ncbi.nlm.nih.gov/pubmed/36059712
http://dx.doi.org/10.3389/fonc.2022.956940
_version_ 1784780794564182016
author Iazzolino, Gaia
Mendibil, Unai
Arnaiz, Blanca
Ruiz-de-Angulo, Ane
Azkargorta, Mikel
Uribe, Kepa B.
Khatami, Neda
Elortza, Felix
Olalde, Beatriz
Gomez-Vallejo, Vanessa
Llop, Jordi
Abarrategi, Ander
author_facet Iazzolino, Gaia
Mendibil, Unai
Arnaiz, Blanca
Ruiz-de-Angulo, Ane
Azkargorta, Mikel
Uribe, Kepa B.
Khatami, Neda
Elortza, Felix
Olalde, Beatriz
Gomez-Vallejo, Vanessa
Llop, Jordi
Abarrategi, Ander
author_sort Iazzolino, Gaia
collection PubMed
description In vitro cell culture studies are common in the cancer research field, and reliable biomimetic 3D models are needed to ensure physiological relevance. In this manuscript, we hypothesized that decellularized xenograft tumors can serve as an optimal 3D substrate to generate a top-down approach for in vitro tumor modeling. Multiple tumor cell lines were xenografted and the formed solid tumors were recovered for their decellularization by several techniques and further characterization by histology and proteomics techniques. Selected decellularized tumor xenograft samples were seeded with the HCC1806 human triple-negative breast cancer (TNBC) basal-like subtype cell line, and cell behavior was compared among them and with other control 2D and 3D cell culture methods. A soft treatment using Freeze-EDTA-DNAse allows proper decellularization of xenografted tumor samples. Interestingly, proteomic data show that samples decellularized from TNBC basal-like subtype xenograft models had different extracellular matrix (ECM) compositions compared to the rest of the xenograft tumors tested. The in vitro recellularization of decellularized ECM (dECM) yields tumor-type–specific cell behavior in the TNBC context. Data show that dECM derived from xenograft tumors is a feasible substrate for reseeding purposes, thereby promoting tumor-type–specific cell behavior. These data serve as a proof-of-concept for further potential generation of patient-specific in vitro research models.
format Online
Article
Text
id pubmed-9434107
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-94341072022-09-02 Decellularization of xenografted tumors provides cell-specific in vitro 3D environment Iazzolino, Gaia Mendibil, Unai Arnaiz, Blanca Ruiz-de-Angulo, Ane Azkargorta, Mikel Uribe, Kepa B. Khatami, Neda Elortza, Felix Olalde, Beatriz Gomez-Vallejo, Vanessa Llop, Jordi Abarrategi, Ander Front Oncol Oncology In vitro cell culture studies are common in the cancer research field, and reliable biomimetic 3D models are needed to ensure physiological relevance. In this manuscript, we hypothesized that decellularized xenograft tumors can serve as an optimal 3D substrate to generate a top-down approach for in vitro tumor modeling. Multiple tumor cell lines were xenografted and the formed solid tumors were recovered for their decellularization by several techniques and further characterization by histology and proteomics techniques. Selected decellularized tumor xenograft samples were seeded with the HCC1806 human triple-negative breast cancer (TNBC) basal-like subtype cell line, and cell behavior was compared among them and with other control 2D and 3D cell culture methods. A soft treatment using Freeze-EDTA-DNAse allows proper decellularization of xenografted tumor samples. Interestingly, proteomic data show that samples decellularized from TNBC basal-like subtype xenograft models had different extracellular matrix (ECM) compositions compared to the rest of the xenograft tumors tested. The in vitro recellularization of decellularized ECM (dECM) yields tumor-type–specific cell behavior in the TNBC context. Data show that dECM derived from xenograft tumors is a feasible substrate for reseeding purposes, thereby promoting tumor-type–specific cell behavior. These data serve as a proof-of-concept for further potential generation of patient-specific in vitro research models. Frontiers Media S.A. 2022-08-18 /pmc/articles/PMC9434107/ /pubmed/36059712 http://dx.doi.org/10.3389/fonc.2022.956940 Text en Copyright © 2022 Iazzolino, Mendibil, Arnaiz, Ruiz-de-Angulo, Azkargorta, Uribe, Khatami, Elortza, Olalde, Gomez-Vallejo, Llop and Abarrategi https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Iazzolino, Gaia
Mendibil, Unai
Arnaiz, Blanca
Ruiz-de-Angulo, Ane
Azkargorta, Mikel
Uribe, Kepa B.
Khatami, Neda
Elortza, Felix
Olalde, Beatriz
Gomez-Vallejo, Vanessa
Llop, Jordi
Abarrategi, Ander
Decellularization of xenografted tumors provides cell-specific in vitro 3D environment
title Decellularization of xenografted tumors provides cell-specific in vitro 3D environment
title_full Decellularization of xenografted tumors provides cell-specific in vitro 3D environment
title_fullStr Decellularization of xenografted tumors provides cell-specific in vitro 3D environment
title_full_unstemmed Decellularization of xenografted tumors provides cell-specific in vitro 3D environment
title_short Decellularization of xenografted tumors provides cell-specific in vitro 3D environment
title_sort decellularization of xenografted tumors provides cell-specific in vitro 3d environment
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9434107/
https://www.ncbi.nlm.nih.gov/pubmed/36059712
http://dx.doi.org/10.3389/fonc.2022.956940
work_keys_str_mv AT iazzolinogaia decellularizationofxenograftedtumorsprovidescellspecificinvitro3denvironment
AT mendibilunai decellularizationofxenograftedtumorsprovidescellspecificinvitro3denvironment
AT arnaizblanca decellularizationofxenograftedtumorsprovidescellspecificinvitro3denvironment
AT ruizdeanguloane decellularizationofxenograftedtumorsprovidescellspecificinvitro3denvironment
AT azkargortamikel decellularizationofxenograftedtumorsprovidescellspecificinvitro3denvironment
AT uribekepab decellularizationofxenograftedtumorsprovidescellspecificinvitro3denvironment
AT khatamineda decellularizationofxenograftedtumorsprovidescellspecificinvitro3denvironment
AT elortzafelix decellularizationofxenograftedtumorsprovidescellspecificinvitro3denvironment
AT olaldebeatriz decellularizationofxenograftedtumorsprovidescellspecificinvitro3denvironment
AT gomezvallejovanessa decellularizationofxenograftedtumorsprovidescellspecificinvitro3denvironment
AT llopjordi decellularizationofxenograftedtumorsprovidescellspecificinvitro3denvironment
AT abarrategiander decellularizationofxenograftedtumorsprovidescellspecificinvitro3denvironment