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A palette of site-specific organelle fluorescent thermometers

Intracellular micro-temperature is closely related to cellular processes. Such local temperature inside cells can be measured by fluorescent thermometers, which are a series of fluorescent materials that convert the temperature information to detectable fluorescence signals. To investigate the intra...

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Detalles Bibliográficos
Autores principales: Liu, Xiao, Yamazaki, Takeru, Kwon, Haw-Young, Arai, Satoshi, Chang, Young-Tae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9434161/
https://www.ncbi.nlm.nih.gov/pubmed/36060107
http://dx.doi.org/10.1016/j.mtbio.2022.100405
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author Liu, Xiao
Yamazaki, Takeru
Kwon, Haw-Young
Arai, Satoshi
Chang, Young-Tae
author_facet Liu, Xiao
Yamazaki, Takeru
Kwon, Haw-Young
Arai, Satoshi
Chang, Young-Tae
author_sort Liu, Xiao
collection PubMed
description Intracellular micro-temperature is closely related to cellular processes. Such local temperature inside cells can be measured by fluorescent thermometers, which are a series of fluorescent materials that convert the temperature information to detectable fluorescence signals. To investigate the intracellular temperature fluctuation in various organelles, it is essential to develop site-specific organelle thermometers. In this study, we develop a new series of fluorescent thermometers, Thermo Greens (TGs), to visualize the temperature change in almost all typical organelles. Through fluorescence lifetime-based cell imaging, it was proven that TGs allow the organelle-specific monitoring of temperature gradients created by external heating. The fluorescence lifetime-based thermometry shows that each organelle experiences a distinct temperature increment which depends on the distance away from the heat source. TGs are further demonstrated in the quantitative imaging of heat production at different organelles such as mitochondria and endoplasmic reticulum in brown adipocytes. To date, TGs are the first palette batch of small molecular fluorescent thermometers that can cover almost all typical organelles. These findings can inspire the development of new fluorescent thermometers and enhance the understanding of thermal biology in the future.
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spelling pubmed-94341612022-09-02 A palette of site-specific organelle fluorescent thermometers Liu, Xiao Yamazaki, Takeru Kwon, Haw-Young Arai, Satoshi Chang, Young-Tae Mater Today Bio Full Length Article Intracellular micro-temperature is closely related to cellular processes. Such local temperature inside cells can be measured by fluorescent thermometers, which are a series of fluorescent materials that convert the temperature information to detectable fluorescence signals. To investigate the intracellular temperature fluctuation in various organelles, it is essential to develop site-specific organelle thermometers. In this study, we develop a new series of fluorescent thermometers, Thermo Greens (TGs), to visualize the temperature change in almost all typical organelles. Through fluorescence lifetime-based cell imaging, it was proven that TGs allow the organelle-specific monitoring of temperature gradients created by external heating. The fluorescence lifetime-based thermometry shows that each organelle experiences a distinct temperature increment which depends on the distance away from the heat source. TGs are further demonstrated in the quantitative imaging of heat production at different organelles such as mitochondria and endoplasmic reticulum in brown adipocytes. To date, TGs are the first palette batch of small molecular fluorescent thermometers that can cover almost all typical organelles. These findings can inspire the development of new fluorescent thermometers and enhance the understanding of thermal biology in the future. Elsevier 2022-08-19 /pmc/articles/PMC9434161/ /pubmed/36060107 http://dx.doi.org/10.1016/j.mtbio.2022.100405 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Full Length Article
Liu, Xiao
Yamazaki, Takeru
Kwon, Haw-Young
Arai, Satoshi
Chang, Young-Tae
A palette of site-specific organelle fluorescent thermometers
title A palette of site-specific organelle fluorescent thermometers
title_full A palette of site-specific organelle fluorescent thermometers
title_fullStr A palette of site-specific organelle fluorescent thermometers
title_full_unstemmed A palette of site-specific organelle fluorescent thermometers
title_short A palette of site-specific organelle fluorescent thermometers
title_sort palette of site-specific organelle fluorescent thermometers
topic Full Length Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9434161/
https://www.ncbi.nlm.nih.gov/pubmed/36060107
http://dx.doi.org/10.1016/j.mtbio.2022.100405
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