Cargando…

miR-31-3p functions as a tumor suppressor by directly targeting GABBR2 in prostate cancer

MicroRNAs are key regulators of gene expression in tumorigenesis. In this study, we investigated the tumor-suppressive function of miR-31-3p. Analysis of the Gene Expression Omnibus database revealed that the expression of miR-31-3p in prostate cancer tissues is lower than that in adjacent normal ti...

Descripción completa

Detalles Bibliográficos
Autores principales: Choi, Sujin, Lee, Soonchul, Han, Young-Hoon, Choi, Junwon, Kim, Isaac, Lee, Jusung, An, Hyun-Ju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9434366/
https://www.ncbi.nlm.nih.gov/pubmed/36059697
http://dx.doi.org/10.3389/fonc.2022.945057
_version_ 1784780853872689152
author Choi, Sujin
Lee, Soonchul
Han, Young-Hoon
Choi, Junwon
Kim, Isaac
Lee, Jusung
An, Hyun-Ju
author_facet Choi, Sujin
Lee, Soonchul
Han, Young-Hoon
Choi, Junwon
Kim, Isaac
Lee, Jusung
An, Hyun-Ju
author_sort Choi, Sujin
collection PubMed
description MicroRNAs are key regulators of gene expression in tumorigenesis. In this study, we investigated the tumor-suppressive function of miR-31-3p. Analysis of the Gene Expression Omnibus database revealed that the expression of miR-31-3p in prostate cancer tissues is lower than that in adjacent normal tissues from patients with prostate cancer. Moreover, miR-31-3p induces apoptosis in DU145, PC-3, and LNCap prostate cancer cells, while those transfected with miR-31-3p exhibit significantly decreased cell proliferation, migration, invasiveness, and tumor sphere-forming ability, as determined using the cell counting kit-8, transwell, and sphere-forming assays. Further analysis revealed that GABBR2 is a direct target of miR-31-3p. Within a DU145 xenograft murine model, intratumoral injection of a miR-31-3p mimic suppresses tumor growth. Taken together, the findings of this study suggest that miR-31-3p performs a novel tumor-suppressive function in prostate cancer and may represent a novel target for anti-prostate cancer miRNA therapeutics.
format Online
Article
Text
id pubmed-9434366
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-94343662022-09-02 miR-31-3p functions as a tumor suppressor by directly targeting GABBR2 in prostate cancer Choi, Sujin Lee, Soonchul Han, Young-Hoon Choi, Junwon Kim, Isaac Lee, Jusung An, Hyun-Ju Front Oncol Oncology MicroRNAs are key regulators of gene expression in tumorigenesis. In this study, we investigated the tumor-suppressive function of miR-31-3p. Analysis of the Gene Expression Omnibus database revealed that the expression of miR-31-3p in prostate cancer tissues is lower than that in adjacent normal tissues from patients with prostate cancer. Moreover, miR-31-3p induces apoptosis in DU145, PC-3, and LNCap prostate cancer cells, while those transfected with miR-31-3p exhibit significantly decreased cell proliferation, migration, invasiveness, and tumor sphere-forming ability, as determined using the cell counting kit-8, transwell, and sphere-forming assays. Further analysis revealed that GABBR2 is a direct target of miR-31-3p. Within a DU145 xenograft murine model, intratumoral injection of a miR-31-3p mimic suppresses tumor growth. Taken together, the findings of this study suggest that miR-31-3p performs a novel tumor-suppressive function in prostate cancer and may represent a novel target for anti-prostate cancer miRNA therapeutics. Frontiers Media S.A. 2022-08-18 /pmc/articles/PMC9434366/ /pubmed/36059697 http://dx.doi.org/10.3389/fonc.2022.945057 Text en Copyright © 2022 Choi, Lee, Han, Choi, Kim, Lee and An https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Choi, Sujin
Lee, Soonchul
Han, Young-Hoon
Choi, Junwon
Kim, Isaac
Lee, Jusung
An, Hyun-Ju
miR-31-3p functions as a tumor suppressor by directly targeting GABBR2 in prostate cancer
title miR-31-3p functions as a tumor suppressor by directly targeting GABBR2 in prostate cancer
title_full miR-31-3p functions as a tumor suppressor by directly targeting GABBR2 in prostate cancer
title_fullStr miR-31-3p functions as a tumor suppressor by directly targeting GABBR2 in prostate cancer
title_full_unstemmed miR-31-3p functions as a tumor suppressor by directly targeting GABBR2 in prostate cancer
title_short miR-31-3p functions as a tumor suppressor by directly targeting GABBR2 in prostate cancer
title_sort mir-31-3p functions as a tumor suppressor by directly targeting gabbr2 in prostate cancer
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9434366/
https://www.ncbi.nlm.nih.gov/pubmed/36059697
http://dx.doi.org/10.3389/fonc.2022.945057
work_keys_str_mv AT choisujin mir313pfunctionsasatumorsuppressorbydirectlytargetinggabbr2inprostatecancer
AT leesoonchul mir313pfunctionsasatumorsuppressorbydirectlytargetinggabbr2inprostatecancer
AT hanyounghoon mir313pfunctionsasatumorsuppressorbydirectlytargetinggabbr2inprostatecancer
AT choijunwon mir313pfunctionsasatumorsuppressorbydirectlytargetinggabbr2inprostatecancer
AT kimisaac mir313pfunctionsasatumorsuppressorbydirectlytargetinggabbr2inprostatecancer
AT leejusung mir313pfunctionsasatumorsuppressorbydirectlytargetinggabbr2inprostatecancer
AT anhyunju mir313pfunctionsasatumorsuppressorbydirectlytargetinggabbr2inprostatecancer