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Metabolic state switches between morning and evening in association with circadian clock in people without diabetes

AIMS/INTRODUCTION: Understanding morning–evening variation in metabolic state is critical for managing metabolic disorders. We aimed to characterize this variation from the viewpoints of insulin secretion and insulin sensitivity, including their relevance to the circadian rhythm. MATERIALS AND METHO...

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Autores principales: Fujimoto, Ruriko, Ohta, Ysuharu, Masuda, Konosuke, Taguchi, Akihiko, Akiyama, Masaru, Yamamoto, Kaoru, Nakabayashi, Hiroko, Nagao, Yuko, Matsumura, Takuro, Hiroshige, Syunsuke, Kajimura, Yasuko, Akashi, Makoto, Tanizawa, Yukio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9434593/
https://www.ncbi.nlm.nih.gov/pubmed/35429128
http://dx.doi.org/10.1111/jdi.13810
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author Fujimoto, Ruriko
Ohta, Ysuharu
Masuda, Konosuke
Taguchi, Akihiko
Akiyama, Masaru
Yamamoto, Kaoru
Nakabayashi, Hiroko
Nagao, Yuko
Matsumura, Takuro
Hiroshige, Syunsuke
Kajimura, Yasuko
Akashi, Makoto
Tanizawa, Yukio
author_facet Fujimoto, Ruriko
Ohta, Ysuharu
Masuda, Konosuke
Taguchi, Akihiko
Akiyama, Masaru
Yamamoto, Kaoru
Nakabayashi, Hiroko
Nagao, Yuko
Matsumura, Takuro
Hiroshige, Syunsuke
Kajimura, Yasuko
Akashi, Makoto
Tanizawa, Yukio
author_sort Fujimoto, Ruriko
collection PubMed
description AIMS/INTRODUCTION: Understanding morning–evening variation in metabolic state is critical for managing metabolic disorders. We aimed to characterize this variation from the viewpoints of insulin secretion and insulin sensitivity, including their relevance to the circadian rhythm. MATERIALS AND METHODS: A total of 14 and 10 people without diabetes were enrolled, and underwent a 75‐g oral glucose tolerance test (OGTT) and hyperinsulinemic‐euglycemic clamp study, respectively. Participants completed the OGTT or hyperinsulinemic‐euglycemic clamp at 08.00 hours and 20.00 hours in random order. Before each study, hair follicles were collected. In mice, phosphorylation levels of protein kinase B were examined in the liver and muscle by western blotting. RESULTS: Glucose tolerance was better at 08 .00 hours, which was explained by the higher 1‐h insulin secretion on OGTT and increased skeletal muscle insulin sensitivity on hyperinsulinemic‐euglycemic clamp. Hepatic insulin sensitivity, estimated by the hepatic insulin resistance index on OGTT, was better at 20.00 hours. The 1‐h insulin secretion and hepatic insulin resistance index correlated significantly with Per2 messenger ribonucleic acid expression. The change (evening value – morning value) in the glucose infusion rate correlated significantly with the change in non‐esterified fatty acid, but not with clock gene expressions. The change in non‐esterified fatty acid correlated significantly with E4bp4 messenger ribonucleic acid expression and the change in cortisol. In mice, phosphorylation of protein kinase B was decreased in the liver and increased in muscle in the beginning of the active period as, expected from the human study. CONCLUSIONS: Glucose metabolism in each tissue differed between the morning and evening, partly reflecting lipid metabolism, clock genes and cortisol levels. Deeper knowledge of these associations might be useful for ameliorating metabolic disorders.
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spelling pubmed-94345932022-09-08 Metabolic state switches between morning and evening in association with circadian clock in people without diabetes Fujimoto, Ruriko Ohta, Ysuharu Masuda, Konosuke Taguchi, Akihiko Akiyama, Masaru Yamamoto, Kaoru Nakabayashi, Hiroko Nagao, Yuko Matsumura, Takuro Hiroshige, Syunsuke Kajimura, Yasuko Akashi, Makoto Tanizawa, Yukio J Diabetes Investig Articles AIMS/INTRODUCTION: Understanding morning–evening variation in metabolic state is critical for managing metabolic disorders. We aimed to characterize this variation from the viewpoints of insulin secretion and insulin sensitivity, including their relevance to the circadian rhythm. MATERIALS AND METHODS: A total of 14 and 10 people without diabetes were enrolled, and underwent a 75‐g oral glucose tolerance test (OGTT) and hyperinsulinemic‐euglycemic clamp study, respectively. Participants completed the OGTT or hyperinsulinemic‐euglycemic clamp at 08.00 hours and 20.00 hours in random order. Before each study, hair follicles were collected. In mice, phosphorylation levels of protein kinase B were examined in the liver and muscle by western blotting. RESULTS: Glucose tolerance was better at 08 .00 hours, which was explained by the higher 1‐h insulin secretion on OGTT and increased skeletal muscle insulin sensitivity on hyperinsulinemic‐euglycemic clamp. Hepatic insulin sensitivity, estimated by the hepatic insulin resistance index on OGTT, was better at 20.00 hours. The 1‐h insulin secretion and hepatic insulin resistance index correlated significantly with Per2 messenger ribonucleic acid expression. The change (evening value – morning value) in the glucose infusion rate correlated significantly with the change in non‐esterified fatty acid, but not with clock gene expressions. The change in non‐esterified fatty acid correlated significantly with E4bp4 messenger ribonucleic acid expression and the change in cortisol. In mice, phosphorylation of protein kinase B was decreased in the liver and increased in muscle in the beginning of the active period as, expected from the human study. CONCLUSIONS: Glucose metabolism in each tissue differed between the morning and evening, partly reflecting lipid metabolism, clock genes and cortisol levels. Deeper knowledge of these associations might be useful for ameliorating metabolic disorders. John Wiley and Sons Inc. 2022-05-18 2022-09 /pmc/articles/PMC9434593/ /pubmed/35429128 http://dx.doi.org/10.1111/jdi.13810 Text en © 2022 The Authors. Journal of Diabetes Investigation published by Asian Association for the Study of Diabetes (AASD) and John Wiley & Sons Australia, Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Articles
Fujimoto, Ruriko
Ohta, Ysuharu
Masuda, Konosuke
Taguchi, Akihiko
Akiyama, Masaru
Yamamoto, Kaoru
Nakabayashi, Hiroko
Nagao, Yuko
Matsumura, Takuro
Hiroshige, Syunsuke
Kajimura, Yasuko
Akashi, Makoto
Tanizawa, Yukio
Metabolic state switches between morning and evening in association with circadian clock in people without diabetes
title Metabolic state switches between morning and evening in association with circadian clock in people without diabetes
title_full Metabolic state switches between morning and evening in association with circadian clock in people without diabetes
title_fullStr Metabolic state switches between morning and evening in association with circadian clock in people without diabetes
title_full_unstemmed Metabolic state switches between morning and evening in association with circadian clock in people without diabetes
title_short Metabolic state switches between morning and evening in association with circadian clock in people without diabetes
title_sort metabolic state switches between morning and evening in association with circadian clock in people without diabetes
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9434593/
https://www.ncbi.nlm.nih.gov/pubmed/35429128
http://dx.doi.org/10.1111/jdi.13810
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