Cargando…
The diversity of the glycan shield of sarbecoviruses closely related to SARS-CoV-2
The animal reservoirs of sarbecoviruses represent a significant risk of emergent pandemics, as evidenced by the impact of SARS-CoV-2. Vaccines remain successful at limiting severe disease and death, however the continued emergence of SARS-CoV-2 variants, together with the potential for further coron...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9435400/ https://www.ncbi.nlm.nih.gov/pubmed/36052375 http://dx.doi.org/10.1101/2022.08.24.505118 |
_version_ | 1784781133987184640 |
---|---|
author | Allen, Joel D. Ivory, Dylan Ge Song, Sophie He, Wan-ting Capozzola, Tazio Yong, Peter Burton, Dennis R. Andrabi, Raiees Crispin, Max |
author_facet | Allen, Joel D. Ivory, Dylan Ge Song, Sophie He, Wan-ting Capozzola, Tazio Yong, Peter Burton, Dennis R. Andrabi, Raiees Crispin, Max |
author_sort | Allen, Joel D. |
collection | PubMed |
description | The animal reservoirs of sarbecoviruses represent a significant risk of emergent pandemics, as evidenced by the impact of SARS-CoV-2. Vaccines remain successful at limiting severe disease and death, however the continued emergence of SARS-CoV-2 variants, together with the potential for further coronavirus zoonosis, motivates the search for pan-coronavirus vaccines that induce broadly neutralizing antibodies. This necessitates a better understanding of the glycan shields of coronaviruses, which can occlude potential antibody epitopes on spike glycoproteins. Here, we compare the structure of several sarbecovirus glycan shields. Many N-linked glycan attachment sites are shared by all sarbecoviruses, and the processing state of certain sites is highly conserved. However, there are significant differences in the processing state at several glycan sites that surround the receptor binding domain. Our studies reveal similarities and differences in the glycosylation of sarbecoviruses and show how subtle changes in the protein sequence can have pronounced impacts on the glycan shield. |
format | Online Article Text |
id | pubmed-9435400 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Cold Spring Harbor Laboratory |
record_format | MEDLINE/PubMed |
spelling | pubmed-94354002022-09-02 The diversity of the glycan shield of sarbecoviruses closely related to SARS-CoV-2 Allen, Joel D. Ivory, Dylan Ge Song, Sophie He, Wan-ting Capozzola, Tazio Yong, Peter Burton, Dennis R. Andrabi, Raiees Crispin, Max bioRxiv Article The animal reservoirs of sarbecoviruses represent a significant risk of emergent pandemics, as evidenced by the impact of SARS-CoV-2. Vaccines remain successful at limiting severe disease and death, however the continued emergence of SARS-CoV-2 variants, together with the potential for further coronavirus zoonosis, motivates the search for pan-coronavirus vaccines that induce broadly neutralizing antibodies. This necessitates a better understanding of the glycan shields of coronaviruses, which can occlude potential antibody epitopes on spike glycoproteins. Here, we compare the structure of several sarbecovirus glycan shields. Many N-linked glycan attachment sites are shared by all sarbecoviruses, and the processing state of certain sites is highly conserved. However, there are significant differences in the processing state at several glycan sites that surround the receptor binding domain. Our studies reveal similarities and differences in the glycosylation of sarbecoviruses and show how subtle changes in the protein sequence can have pronounced impacts on the glycan shield. Cold Spring Harbor Laboratory 2022-08-25 /pmc/articles/PMC9435400/ /pubmed/36052375 http://dx.doi.org/10.1101/2022.08.24.505118 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator. |
spellingShingle | Article Allen, Joel D. Ivory, Dylan Ge Song, Sophie He, Wan-ting Capozzola, Tazio Yong, Peter Burton, Dennis R. Andrabi, Raiees Crispin, Max The diversity of the glycan shield of sarbecoviruses closely related to SARS-CoV-2 |
title | The diversity of the glycan shield of sarbecoviruses closely related to SARS-CoV-2 |
title_full | The diversity of the glycan shield of sarbecoviruses closely related to SARS-CoV-2 |
title_fullStr | The diversity of the glycan shield of sarbecoviruses closely related to SARS-CoV-2 |
title_full_unstemmed | The diversity of the glycan shield of sarbecoviruses closely related to SARS-CoV-2 |
title_short | The diversity of the glycan shield of sarbecoviruses closely related to SARS-CoV-2 |
title_sort | diversity of the glycan shield of sarbecoviruses closely related to sars-cov-2 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9435400/ https://www.ncbi.nlm.nih.gov/pubmed/36052375 http://dx.doi.org/10.1101/2022.08.24.505118 |
work_keys_str_mv | AT allenjoeld thediversityoftheglycanshieldofsarbecovirusescloselyrelatedtosarscov2 AT ivorydylan thediversityoftheglycanshieldofsarbecovirusescloselyrelatedtosarscov2 AT gesongsophie thediversityoftheglycanshieldofsarbecovirusescloselyrelatedtosarscov2 AT hewanting thediversityoftheglycanshieldofsarbecovirusescloselyrelatedtosarscov2 AT capozzolatazio thediversityoftheglycanshieldofsarbecovirusescloselyrelatedtosarscov2 AT yongpeter thediversityoftheglycanshieldofsarbecovirusescloselyrelatedtosarscov2 AT burtondennisr thediversityoftheglycanshieldofsarbecovirusescloselyrelatedtosarscov2 AT andrabiraiees thediversityoftheglycanshieldofsarbecovirusescloselyrelatedtosarscov2 AT crispinmax thediversityoftheglycanshieldofsarbecovirusescloselyrelatedtosarscov2 AT allenjoeld diversityoftheglycanshieldofsarbecovirusescloselyrelatedtosarscov2 AT ivorydylan diversityoftheglycanshieldofsarbecovirusescloselyrelatedtosarscov2 AT gesongsophie diversityoftheglycanshieldofsarbecovirusescloselyrelatedtosarscov2 AT hewanting diversityoftheglycanshieldofsarbecovirusescloselyrelatedtosarscov2 AT capozzolatazio diversityoftheglycanshieldofsarbecovirusescloselyrelatedtosarscov2 AT yongpeter diversityoftheglycanshieldofsarbecovirusescloselyrelatedtosarscov2 AT burtondennisr diversityoftheglycanshieldofsarbecovirusescloselyrelatedtosarscov2 AT andrabiraiees diversityoftheglycanshieldofsarbecovirusescloselyrelatedtosarscov2 AT crispinmax diversityoftheglycanshieldofsarbecovirusescloselyrelatedtosarscov2 |