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Structure of angiogenin dimer bound to double-stranded RNA
Angiogenin is an unusual member of the RNase A family and is of great interest in multiple pathological contexts. Although it has been assigned various regulatory roles, its core catalytic function is that of an RNA endonuclease. However, its catalytic efficiency is comparatively low and this has be...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9435672/ https://www.ncbi.nlm.nih.gov/pubmed/36048083 http://dx.doi.org/10.1107/S2053230X22008317 |
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author | Sievers, Katharina Ficner, Ralf |
author_facet | Sievers, Katharina Ficner, Ralf |
author_sort | Sievers, Katharina |
collection | PubMed |
description | Angiogenin is an unusual member of the RNase A family and is of great interest in multiple pathological contexts. Although it has been assigned various regulatory roles, its core catalytic function is that of an RNA endonuclease. However, its catalytic efficiency is comparatively low and this has been linked to a unique C-terminal helix which partially blocks its RNA-binding site. Assuming that binding to its RNA substrate could trigger a conformational rearrangement, much speculation has arisen on the topic of the interaction of angiogenin with RNA. To date, no structural data on angiogenin–RNA interactions have been available. Here, the structure of angiogenin bound to a double-stranded RNA duplex is reported. The RNA does not reach the active site of angiogenin and no structural arrangement of the C-terminal domain is observed. However, angiogenin forms a previously unobserved crystallographic dimer that makes several backbone interactions with the major and minor grooves of the RNA double helix. |
format | Online Article Text |
id | pubmed-9435672 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-94356722022-10-03 Structure of angiogenin dimer bound to double-stranded RNA Sievers, Katharina Ficner, Ralf Acta Crystallogr F Struct Biol Commun Research Communications Angiogenin is an unusual member of the RNase A family and is of great interest in multiple pathological contexts. Although it has been assigned various regulatory roles, its core catalytic function is that of an RNA endonuclease. However, its catalytic efficiency is comparatively low and this has been linked to a unique C-terminal helix which partially blocks its RNA-binding site. Assuming that binding to its RNA substrate could trigger a conformational rearrangement, much speculation has arisen on the topic of the interaction of angiogenin with RNA. To date, no structural data on angiogenin–RNA interactions have been available. Here, the structure of angiogenin bound to a double-stranded RNA duplex is reported. The RNA does not reach the active site of angiogenin and no structural arrangement of the C-terminal domain is observed. However, angiogenin forms a previously unobserved crystallographic dimer that makes several backbone interactions with the major and minor grooves of the RNA double helix. International Union of Crystallography 2022-08-30 /pmc/articles/PMC9435672/ /pubmed/36048083 http://dx.doi.org/10.1107/S2053230X22008317 Text en © Sievers and Ficner 2022 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Research Communications Sievers, Katharina Ficner, Ralf Structure of angiogenin dimer bound to double-stranded RNA |
title | Structure of angiogenin dimer bound to double-stranded RNA |
title_full | Structure of angiogenin dimer bound to double-stranded RNA |
title_fullStr | Structure of angiogenin dimer bound to double-stranded RNA |
title_full_unstemmed | Structure of angiogenin dimer bound to double-stranded RNA |
title_short | Structure of angiogenin dimer bound to double-stranded RNA |
title_sort | structure of angiogenin dimer bound to double-stranded rna |
topic | Research Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9435672/ https://www.ncbi.nlm.nih.gov/pubmed/36048083 http://dx.doi.org/10.1107/S2053230X22008317 |
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