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High infectivity and unique genomic sequence characteristics of Cryptosporidium parvum in China
Zoonotic Cryptosporidium parvum infections are mainly caused by IIa and IId subtypes. As most biological characterizations have been performed on IIa subtypes, the biological and genetic characteristics of IId subtypes in China are not clear. We evaluated the infection and genetic characteristics of...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9436107/ https://www.ncbi.nlm.nih.gov/pubmed/35994488 http://dx.doi.org/10.1371/journal.pntd.0010714 |
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author | Jia, Ruilian Huang, Wanyi Huang, Ni Yu, Zhengjie Li, Na Xiao, Lihua Feng, Yaoyu Guo, Yaqiong |
author_facet | Jia, Ruilian Huang, Wanyi Huang, Ni Yu, Zhengjie Li, Na Xiao, Lihua Feng, Yaoyu Guo, Yaqiong |
author_sort | Jia, Ruilian |
collection | PubMed |
description | Zoonotic Cryptosporidium parvum infections are mainly caused by IIa and IId subtypes. As most biological characterizations have been performed on IIa subtypes, the biological and genetic characteristics of IId subtypes in China are not clear. We evaluated the infection and genetic characteristics of IId isolates in interferon-γ-knockout mice using qPCR to quantify oocyst shedding, histological examination to monitor pathological changes and comparative genomic analyses to identify infectivity and virulence-associated differences. Compared with the reference IIa isolate, mice infected with the IId isolates had significantly higher and longer oocyst shedding and lower body weight gain. In addition, the four IId isolates examined differed significantly in infectivity (as indicated by the median infective dose), oocyst shedding duration, and pathogenicity. Comparative genomic analysis indicated that the IId isolates had three more subtelomeric genes than the reference IIa isolate and 5385–5548 nucleotide substitutions, with the hypervariable genes mostly in two blocks on chromosome 1. In contrast, the four IId isolates differed from each other by 77–1,452 nucleotides, with virulence-associated sequence differences mainly in nine genes within a 28-kb block on chromosome 6. These data indicate the newly emerged C. parvum IId subtypes in China have high animal infectivity and unique genomic characteristics. |
format | Online Article Text |
id | pubmed-9436107 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-94361072022-09-02 High infectivity and unique genomic sequence characteristics of Cryptosporidium parvum in China Jia, Ruilian Huang, Wanyi Huang, Ni Yu, Zhengjie Li, Na Xiao, Lihua Feng, Yaoyu Guo, Yaqiong PLoS Negl Trop Dis Research Article Zoonotic Cryptosporidium parvum infections are mainly caused by IIa and IId subtypes. As most biological characterizations have been performed on IIa subtypes, the biological and genetic characteristics of IId subtypes in China are not clear. We evaluated the infection and genetic characteristics of IId isolates in interferon-γ-knockout mice using qPCR to quantify oocyst shedding, histological examination to monitor pathological changes and comparative genomic analyses to identify infectivity and virulence-associated differences. Compared with the reference IIa isolate, mice infected with the IId isolates had significantly higher and longer oocyst shedding and lower body weight gain. In addition, the four IId isolates examined differed significantly in infectivity (as indicated by the median infective dose), oocyst shedding duration, and pathogenicity. Comparative genomic analysis indicated that the IId isolates had three more subtelomeric genes than the reference IIa isolate and 5385–5548 nucleotide substitutions, with the hypervariable genes mostly in two blocks on chromosome 1. In contrast, the four IId isolates differed from each other by 77–1,452 nucleotides, with virulence-associated sequence differences mainly in nine genes within a 28-kb block on chromosome 6. These data indicate the newly emerged C. parvum IId subtypes in China have high animal infectivity and unique genomic characteristics. Public Library of Science 2022-08-22 /pmc/articles/PMC9436107/ /pubmed/35994488 http://dx.doi.org/10.1371/journal.pntd.0010714 Text en © 2022 Jia et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Jia, Ruilian Huang, Wanyi Huang, Ni Yu, Zhengjie Li, Na Xiao, Lihua Feng, Yaoyu Guo, Yaqiong High infectivity and unique genomic sequence characteristics of Cryptosporidium parvum in China |
title | High infectivity and unique genomic sequence characteristics of Cryptosporidium parvum in China |
title_full | High infectivity and unique genomic sequence characteristics of Cryptosporidium parvum in China |
title_fullStr | High infectivity and unique genomic sequence characteristics of Cryptosporidium parvum in China |
title_full_unstemmed | High infectivity and unique genomic sequence characteristics of Cryptosporidium parvum in China |
title_short | High infectivity and unique genomic sequence characteristics of Cryptosporidium parvum in China |
title_sort | high infectivity and unique genomic sequence characteristics of cryptosporidium parvum in china |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9436107/ https://www.ncbi.nlm.nih.gov/pubmed/35994488 http://dx.doi.org/10.1371/journal.pntd.0010714 |
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